2004
DOI: 10.1016/j.ecoenv.2004.06.002
|View full text |Cite
|
Sign up to set email alerts
|

Phenotypic yeast growth analysis for chronic toxicity testing

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
21
0

Year Published

2005
2005
2013
2013

Publication Types

Select...
8
1

Relationship

2
7

Authors

Journals

citations
Cited by 38 publications
(21 citation statements)
references
References 51 publications
0
21
0
Order By: Relevance
“…Given the principal advantages of yeasts as test organisms, it seemed important to us to thoroughly investigate the sensitivity of yeast to highly diluted and succussed substances. Both species examined here are well studied and often used in industrial and medical biotechnology as well as biological models and sensitive test organisms in environmental studies [44][45][46]. The aim of this study was thus to investigate if potentised substances show any effects on growth kinetics of two different yeast species in a stable, reliable test system with systematic negative controls.…”
Section: Introductionmentioning
confidence: 99%
“…Given the principal advantages of yeasts as test organisms, it seemed important to us to thoroughly investigate the sensitivity of yeast to highly diluted and succussed substances. Both species examined here are well studied and often used in industrial and medical biotechnology as well as biological models and sensitive test organisms in environmental studies [44][45][46]. The aim of this study was thus to investigate if potentised substances show any effects on growth kinetics of two different yeast species in a stable, reliable test system with systematic negative controls.…”
Section: Introductionmentioning
confidence: 99%
“…The effects of Co on the growth of microorganisms have been studied by many researchers. [42][43][44][45][46][47] Cobalt is suggested to be accumulated in yeast cells and be stored in vacuoles. 48,49) Norris and Kelly 50) indicated that Co 2þ is presumably adsorbed on the cell surface in the first step, followed by metabolism-dependent intracellular uptake.…”
Section: Discussionmentioning
confidence: 99%
“…We have previously described the construction and preliminary characterization of chronic toxicity and genotoxicity test systems in sensitive S. cerevisiae host strains (21,33,34). We report here on the extension of the assay toward the use of the PMA1 promoter (P PMA1 ) of S. cerevisiae as a biomarker for the rapid detection of toxicant-induced stress on basic metabolism.…”
mentioning
confidence: 99%
“…The proton motive force is responsible for secondary active transport mechanisms for a variety of nutrients and is also involved in pH homeostasis. In being the major protein component of the plasma membrane (15 to 20% of total plasma membrane protein [1]), expression and activity of this proton pump are precisely regulated to match its numerous requirements (4,11,19,38).We have previously described the construction and preliminary characterization of chronic toxicity and genotoxicity test systems in sensitive S. cerevisiae host strains (21,33,34). We report here on the extension of the assay toward the use of the PMA1 promoter (P PMA1 ) of S. cerevisiae as a biomarker for the rapid detection of toxicant-induced stress on basic metabolism.…”
mentioning
confidence: 99%