Phenotypical Characteristics of Peripheral Blood Monocytes in Normal Pregnancy and Gestosis

Abstract

Monocyte migration from the peripheral blood to the uterine decidual tissue is essential for the regulation of placental development. We evaluated the phenotypical changes in the peripheral blood monocytes in pregnant women. The peripheral blood counts of monocytes expressing CD11b, CD47, and integrin β7 were elevated in women with normal gestation in comparison with nonpregnant women; the intensity of CD62P, CD11b, CD11c, CD29, CD31, and CD54 expression was higher in pregnancy. The counts of monocytes expressing adhesion molecules were similar in normal pregnancy and gestosis. Gestosis was characterized by higher counts of monocytes expressing IFN-γ receptor (CD119) and more intense expression of this receptor. Changes in the monocyte phenotype can promote their adhesion to the uterine vascular endothelium and further migration of these cells to the decidual tissue.

Influence of peripheral blood microparticles of pregnant women with preeclampsia on the phenotype of monocytes

“…Nevertheless, this number was not different from the number of endothelial-derived microparticles in women with preeclampsia. 40 The number of microparticles formed by NK-cells was higher in women with normal pregnancy than in nonpregnant women and women with preeclampsia. Women with preeclampsia had the increased number of microparticles with a phenotype typical for neutrophils and monocytes, compared to women with normal pregnancy.…”

“…preeclampsia can be based on the above mentioned differences in the amount and the composition of microparticles. 40,41 In this way, it was indicated that the total number of peripheral blood microparticles of women with normal pregnancy was significantly greater than the total number of peripheral blood microparticles of healthy nonpregnant women. No differences were noticed between the groups of pregnant women with preeclampsia and women without this disease.…”

“…In addition to this, the change in composition of adhesion and activating molecules on peripheral blood monocytes of pregnant women occurs. 3 Platelet-derived and endothelial-derived microparticles influence the composition of adhesion molecules on the surface of THP-1 monocytes and induce production of pro-inflammatory cytokines. 12 In order to understand the role the blood plasma microparticles of pregnant women play in the regulation of the activity of circulating monocytes, it is necessary to take into account a set of changes made in the cell surface receptor's profile at different functional states.…”

“…It was indicated that women with normal pregnancy have an increased expression level of monocyte adhesion molecules such as CD62P, CD11b, CD11c, CD29, CD47, CD31, CD54, and such receptors as CD119 (for IFNγ), CD196, CXCR5 (in comparison with nonpregnant women). 3 Furthermore, during normal pregnancy, monocytes are characterized by a higher level of IL-12, IL-1β and reactive oxygen species production. 2,4 Blood plasma microparticles, the composition of which is changed during pregnancy 7 and complications in pregnancy, 8 can be one of the factors that change surface receptor expression on circulating monocytes.…”