Phenotypically distinguishing ESBL-producing pathogens using paper-based surface enhanced Raman sensors

Hilton, Shannon Huey; Hall, C. J.; Nguyen, Hieu; Everitt, Micaela L.; DeShong, Philip; White, Ian M.

doi:10.1016/j.aca.2020.06.068

Cited by 20 publications

(19 citation statements)

References 45 publications

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“…Single cell RS has already been used to identify Mycobacterium tuberculosis and yeast species (11, 12). In this study, the characteristic peaks of Elizabethkingia spp.…”

Section: Discussionmentioning

confidence: 99%

“…Single cell RS provides a biochemical "fingerprint" of individual cell, which were reflected cell physiological and metabolic states (6). It was applied to identify bacterial strains (7)(8)(9)(10) and to detect physiological changes during the treatment of antimicrobials (11,12). It has been reported that different Raman spectra were consisted in Listeria monocytogenes with different susceptibilities to sakacin P in 2006 (13).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…It has been discovered that the metabolically active microorganisms could incorporate the deuterium (D) into the cells via NADH/NADPH electron transport chain, producing a newly formed carbon-deuterium (C-D) band in single cell RS analysis coupling with deuterium probing (single cell Raman-DIP) (5, 6, 21, 22). The occurrence of C-D band around 2040-2300 cm −1 has been recognized as antimicrobial resistance biomarker when bacteria were exposed to antimicrobials and D 2 O (11). Recently, single cell Raman-DIP was proposed to achieve fast AST for pathogens such as Escherichia coli , Klebsiella pneumoniae , Streptococcus mutans, Lactobacillus fermentum, Enterococcus faecalis and Staphylococcus aureus (5, 23–26).…”

Section: Introductionmentioning

confidence: 99%

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Single Cell Raman-Deuterium Isotope Probing for Drug Resistance of Elizabethkingia spp

Yuan

Chen

Song

et al. 2021

Preprint

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Nosocomial infection associated with Elizabethkingia spp. is an emerging clinical concern characterized by multi-drug resistance and severe clinical consequences particularly in immunocompromised individuals and infants. Efficient control of this infection demands quick and reliable methods to determine the right drugs for the treatment. In this study, E. meningoseptica ATCC 13253 and four clinical isolates of Elizabethkingia spp. obtained from China, were subjected to single cell Raman spectroscopy analysis coupling with deuterium probing (single cell Raman-DIP). The results demonstrated that single cell Raman-DIP could generate an antimicrobial susceptibility testing result for Elizabethkingia spp. colonies within 4 hours based on their metabolisms variations at single cell level, and the drug resistant spectra of Elizabethkingia spp. determined by single cell Raman-DIP were consistent with the classical MIC method. Meanwhile single cell Raman spectroscopy (single cell RS) was applied to analyze Raman spectra of Elizabethkingia spp., which were revealed that their ratios of nucleic acid/protein were lower than other gram-negative pathogens and isolates from different origins could be distinguished by their Raman fingerprint. The in vitro results confirmed that minocycline and levofloxacin are first-line antimicrobials for Elizabethkingia spp. infection.

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“…Single cell RS has already been used to identify Mycobacterium tuberculosis and yeast species (11, 12). In this study, the characteristic peaks of Elizabethkingia spp.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Single Cell Raman-Deuterium Isotope Probing for Drug Resistance of Elizabethkingia spp

Yuan

Chen

Song

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…10,11 Emerging diagnostic platforms are employing microfluidics, biosensors, and lab-on-a-chip technology for genotypic and phenotypic detection of β-lactamases. [12][13][14] Rapid phenotypic ASTs hybridized with microfluidic chips and biosensors measuring optical, 15 chemical, [16][17][18] electrical, [19][20][21][22] mechanical, 23,24 or other 25,26 signals during bacteria growth in the presence of β-lactam antibiotics have been demonstrated even with very low numbers of bacteria and sample volumes and in parallel testing of different pathogens with multiple antibiotics. While holding great potentials, their robustness, costeffectiveness, and adaptability into large scale clinical setting remains to be further illustrated.…”

mentioning

confidence: 99%