Phenotypically Mixed Vesicular Stomatitis Virus Particles Produced in Human Tumor Cell Lines

Biological and electron microscopic studies on the phenotypic mixing of the thermolabile mutant of vesicular stomatitis virus,tl-17, with avian RNA tumor viruses

“…Furthermore, the heat resistant fraction had the host range of the ATV, used in the phenotypic mixing. The localization of heat resistant projections as investigated by electron microscopy was different from the model presented by others (18,23). These authors suggested that the projections of VSV and ATV were arranged alternatively on the surface of VSV, forming mosaic virus.…”

“…Though it was impossible to distinguish the spikes of VSV and ATV morphologically from each other, those remaining projections most probably are ATV specific. These ATV specific projections on the surface of VSV were never distributed sparsely over the whole virion, ~s one might expect if the VSV and ATV projections were arranged alternately, forming mosaic virus as was suggested by ZXVADA et al (23) and WEiss et al (18). Instead, the ATV specific projections appeared in ehsters at the apical, body or tail regions of VSV.…”

A Vesicular Stomatitis Virus (Cytomegalovirus) Pseudotype and Its Use in Neutralization Tests

“…First, it may serve as a probe for revealing the presence of viral antigens in cells synthesizing these antigens but no infectious progeny. It has been demonstrated beyond doubt that pseudotypes containing the VSV genome can be produced in such cells [2], and they are uti lized in the search for putative human on cornaviruses [3], Pseudotypes can also serve as useful tools in neutralization tests (NT) for antigens from noncytopathogenic or slowly growing viruses. In this respect, VSV offers special advantages.…”