Phenotyping of Lewis and secretor HBGA from saliva and detection of new FUT2 gene SNPs from young children from the Amazon presenting acute gastroenteritis and respiratory infection

Moraes, Marcia Terezinha Baroni de; Olivares, Alberto Ignácio Olivares; Fialho, Alexandre Madi; Malta, Fábio Correia; Mouta, Sergio da Silva e; Bispo, Romanul de Souza; Velloso, Alvaro Jorge; Leitão, Gabriel Azevedo Alves; Cantelli, Carina Pacheco; Nordgren, Johan; Svenson, Lennart; Miagostovich, Marize Pereira; Leite, José Paulo Gagliardi

doi:10.1016/j.meegid.2019.02.011

Cited by 13 publications

(19 citation statements)

References 27 publications

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“…Positive secretor status was the most prevalent (80.3%) in this community-cohort, and the Le (a+b+) profile (59.8%) frequency was similar to that observed in younger children from the Amazon (58.8%) as presented in our previous study 44 . The rs1047781 (385 A > T) SNP, responsible for the weak genotype in the Asian population, was not detected in Le (a+b+) children in the Manguinhos community, Rio de Janeiro state.…”

Section: Discussionsupporting

confidence: 88%

“…The rs1047781 (385 A > T) SNP, responsible for the weak genotype in the Asian population, was not detected in Le (a+b+) children in the Manguinhos community, Rio de Janeiro state. The rs281377 (357 C > T), a synonymous mutation, was the most frequent SNP detected in this Le (a+b+) secretor profile, and this SNP has been detected in the Brazilian population 44,45 . According to Ferrer-Admetlla et al 19 , the rs281377 (357 C > T) SNP has been detected in a worldwide distribution profile in the natural population, and the se 357/385 haplotype is frequently detected in the Asian population.…”

Section: Discussionmentioning

confidence: 85%

“…The Manguinhos cohort showed a high prevalence of secretors, being a common profile in Latin America 30,31,44,45,49 . In this study, we were able to investigate RVA shedding and a common mutation was identified in many samples characterized as RV1 according to VP8* Sanger sequencing criteria.…”

Section: Discussionmentioning

confidence: 99%

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Rotavirus A shedding and HBGA host genetic susceptibility in a birth community-cohort, Rio de Janeiro, Brazil, 2014–2018

Cantelli

Velloso²,

Assis

et al. 2020

Sci Rep

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Recent studies have investigated whether the human histo-blood group antigen (HBGAs) could affect the effectiveness of the oral rotavirus vaccines, suggesting secretor positive individuals develop a more robust response. We investigated the Rotavirus A (RVA) shedding in association with the host susceptibility profile in children from a birth community-cohort in Rio de Janeiro, Brazil, from 2014 to 2018. A total of 132 children were followed-up between 0 to 11-month-old, stool samples were collected before/after the 1 st /2 nd RV1 vaccination doses and saliva samples were collected during the study. RVA shedding was screened by RT-qPCR and G/P genotypes determined by multiplex RT-PCR and/or Sanger nucleotide sequencing. The sequencing indicated an F167L amino acid change in the RV1 VP8* P[8] in 20.5% of shedding follow-ups and these mutant subpopulations were quantified by pyrosequencing. The HBGA/secretor status was determined and 80.3% of the children were secretors. Twenty-one FUT2 gene SNPs were identified and two new mutations were observed. The mutant F167L RV1 VP8* P[8] was detected significantly more in Le (a+b+) secretors (90.5%) compared to non-secretors and even to secretors Le (a−b+) (9.5%). The study highlights the probable association between RV1 shedding and HBGAs as a marker for evaluating vaccine strain host susceptibility. After implementation of the rotavirus vaccines, Rotarix ® (RV1, GlaxoSmithKline Biologicals, Rixensart, Belgium) and RotaTeq ® (RV5, Merck Inc., USA), in over 100 countries worldwide 1 , the burden of severe group A rotaviruses (RVA) diarrhea has decreased substantially, with reductions in hospitalizations and deaths in many countries, including Brazil 2-6. However, RVA still are one of major causes of severe viral diarrhea in infants and young children <5-years-old worldwide 6,7. Due to the zoonotic potential, variability of the RVA strains and host genetic factors, the surveillance of circulating RVA strains is necessary for evaluating and monitoring the effectiveness of the implemented immunization programs, mainly in low-income countries 8. Currently, 36 G-and 51 P-genotypes have been described 9 and globally, six G/P combinations are the most prevalent in humans: G1P[8], G2P[4], G3P[8], G9P[8], G4P[8] and G12P[8] 10 .

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 85%

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Rotavirus A shedding and HBGA host genetic susceptibility in a birth community-cohort, Rio de Janeiro, Brazil, 2014–2018

Cantelli

Velloso²,

Assis

et al. 2020

Sci Rep

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“…The 82% of secretors in the control group found in the present study were therefore consistent with these previous observations. The most common FUT2 mutation, G428A, was observed in all non-secretor children in our study, which contrasts with a recent study performed in North of South America with Amazonian children from Brazil, Venezuela, and English Guyana, where this common FUT2 gene mutation was not detected [ 50 ]. These results highlight the importance of studying the influence of FUT2 polymorphisms on susceptibility to enteric viruses in populations of different geographic locations, such as those from Latin America.…”

Section: Discussioncontrasting

confidence: 99%

“…Although the frequency of the secretor phenotype varies across populations, approximately 80% of the world population are secretors [ 16 ]. In Brazil, the prevalence of this phenotype revealed in the few studies, is somewhat similar, ranging from 75% to 90% [ 28 , 49 , 50 , 51 ]. The 82% of secretors in the control group found in the present study were therefore consistent with these previous observations.…”

Section: Discussionmentioning

confidence: 98%

FUT2, Secretor Status and FUT3 Polymorphisms of Children with Acute Diarrhea Infected with Rotavirus and Norovirus in Brazil

Tonini

Barreira

Santolin

et al. 2020

Viruses

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Host susceptibility according to human histo-blood group antigens (HBGAs) is widely known for norovirus infection, but is less described for rotavirus. Due to the variable HBGA polymorphism among populations, we aimed to evaluate the association between HBGA phenotypes (ABH, Lewis and secretor status) and susceptibility to rotavirus and norovirus symptomatic infection, and the polymorphisms of FUT2 and FUT3, of children from southeastern Brazil. Paired fecal-buccal specimens from 272 children with acute diarrhea were used to determine rotavirus/norovirus genotypes and HBGAs phenotypes/genotypes, respectively. Altogether, 100 (36.8%) children were infected with rotavirus and norovirus. The rotavirus P[8] genotype predominates (85.7%). Most of the noroviruses (93.8%) belonged to genogroup II (GII). GII.4 Sydney represented 76% (35/46) amongst five other genotypes. Rotavirus and noroviruses infected predominantly children with secretor status (97% and 98.5%, respectively). However, fewer rotavirus-infected children were Lewis-negative (8.6%) than the norovirus-infected ones (18.5%). FUT3 single nucleotide polymorphisms (SNP) occurred mostly at the T59G > G508A > T202C > C314T positions. Our results reinforce the current knowledge that secretors are more susceptible to infection by both rotavirus and norovirus than non-secretors. The high rate for Lewis negative (17.1%) and the combination of SNPs, beyond the secretor status, may reflect the highly mixed population in Brazil.

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First report of human astrovirus MLB2 in Brazil detected in feces of children with acute gastroenteritis living in the state of Roraima, Northern Brazil

Olivares

Moraes

Queiroz

et al. 2020

The Brazilian Journal of Infectious Diseases

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Phenotyping of Lewis and secretor HBGA from saliva and detection of new FUT2 gene SNPs from young children from the Amazon presenting acute gastroenteritis and respiratory infection

Cited by 13 publications

References 27 publications

Rotavirus A shedding and HBGA host genetic susceptibility in a birth community-cohort, Rio de Janeiro, Brazil, 2014–2018

Rotavirus A shedding and HBGA host genetic susceptibility in a birth community-cohort, Rio de Janeiro, Brazil, 2014–2018

FUT2, Secretor Status and FUT3 Polymorphisms of Children with Acute Diarrhea Infected with Rotavirus and Norovirus in Brazil

First report of human astrovirus MLB2 in Brazil detected in feces of children with acute gastroenteritis living in the state of Roraima, Northern Brazil

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