Phenotyping of pig α₁B‐glycoprotein (PO2) and haemopexin by 1D polyacrylamide gel electrophoresis and immunoblotting

Abstract

Described is an alternative procedure for the phenotyping of pig alB-glycoprotein (P02) and haemopexin. The procedure is based on the separation of serum samples by horizontal polyacrylamide gel electrophoresis, passive blotting onto a nitrocellulose (NC) sheet, and immunochemical detection using a mixture of a primary antibody (rabbit anti-pig a l B or anti-pig haemopexin) and a peroxidaselabelled secondary antibody. Several NC copies can be obtained from a single gel and these can be developed with different monospecific antisera.