Phenotyping of vibrios isolated from marine shrimp hemolymph

Abstract

ABSTRACT. The phenotypic characterization of 100 Vibrio strains isolated from the hemolymph of cultured Pacific white shrimp, Litopenaeus vannamei, was performed. This process allowed the identification of 10 species: V. navarrensis (n = 53), V. brasiliensis (n = 15), V. parahaemolyticus (n = 10), V. xuii (n = 8), V. cholerae (n = 5), V. coralliilyticus (n = 4), V. neptunis (n = 2), V. alginolyticus (n = 1), V. diazotrophicus (n = 1), and V. vulnificus B3 (n = 1). These results suggest that penaeid hemolymph may be colonized by vibrios with no imposition or necessity of pathological compromising.Key words: Vibrio, phenotypic characterization, hemolymph. RESUMEN. Se realizó la caracterización fenotípica de 100 cepas de Vibrio aisladas de la hemolinfa de camarones Litopenaeus vannameicultivados. Este proceso permitió la identificación de 10 especies: V. navarrensis (n = 53), V. brasiliensis (n = 15), V. parahaemolyticus (n = 10), V. xuii (n = 8), V. cholerae (n = 5), V. coralliilyticus (n = 4), V. neptunis (n = 2), V. alginolyticus (n = 1), V. diazotrophicus (n = 1) y V. vulnificus B3 (n = 1). Los resultados sugieren que la hemolinfa de los peneidos puede ser colonizada por vibrios sin comprometer la salud animal.Palabras clave: Vibrio, caracterización fenotípica, hemolinfa.

Antibiotic-Resistant Vibrios in Farmed Shrimp

“…For the genus Vibrio , the phenotypic identification is still considered not enough, since many species can be misidentified. However, the identification of all strains used was validated [ 9 ] and previously published [ 8 ].…”

“…A total of 100 Vibrio strains from the bacterial collection of the Microbiology Laboratory at the Environmental and Fish-Sea Sciences Institute (LABOMAR-UFC) were used in this experiment. Only strains previously identified by phenotyping were used [ 8 ]. The 100 strains, isolated from the hemolymph of Litopenaeus vannamei , were subjected to identification by a set of biochemical keys [ 9 ], using the following tests: arginine dihydrolase, lysine decarboxylase and ornithine decarboxylase, oxidase, indole, ortho -nitrophenyl- β -galactoside (ONPG), Voges-Proskauer, D-glucosamine cs, growth at 0%, 3%, 8%, and 10% NaCl, growth at 40° and 4°C, citrate, gelatinase, urease, resistance to O/129, and acid from glucose, sucrose, arabinose, mannitol, and melibiose.…”

Crustacean hemolymph microbiota: Endemic, tightly controlled, and utilization expectable

“…V. parahaemolyticus is also the dominant species in healthy blue crab hemolymph . In another study performed in L. vannamei, Vibrio navarrensis was found as the dominant stain instead of V. parahaemolyticus; moreover, V. navarrensis corresponded to more than half of the total number of isolates (Albuquerque- Costa et al, 2013). Variation of hemolymph bacterial community, even in similar species, further suggested the influence of external endemic conditions.…”

A glimpse towards cultivable hemolymph microbiota of marine crabs: Untapped resource for aquatic probiotics/antibacterial agents

“…Abundance of vibrios was in the order of C. lucifera > C. feriatus > P. pelagicus > P. sanguinolentus. While no data is available for comparison in same crab species, density of 10 2 -10 3 CFU/ml and 30-460 CFU/ml had been reported in hemolymph of Litopenaeus vannamei (Gomez-Gil et al, 1998;Albuquerque-Costa et al, 2013). More notably, TCBS count of C. lucifera was comparable to the count in non-selective media proposing that most of hemolymph isolates belonged to Vibrionacea.…”

“…Since Vibrio sp. are reported as the dominant bacteria in hemolymph microbiota of marine animals (Colwell et al, 1975;Antunes et al, 2010;Albuquerque-Costa et al, 2013;Wang et al, 2014) specific enumeration for viable presumptive vibrios was done (Bolinches et al, 1988). Abundance of vibrios was in the order of C. lucifera > C. feriatus > P. pelagicus > P. sanguinolentus.…”