Phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits

Flores, Gema; Moreno, Fernando de la Peña; Blanch, Gracia Patricia; Castillo, Marı́a Luisa Ruiz del

doi:10.1016/j.foodchem.2013.12.034

Cited by 19 publications

(9 citation statements)

References 24 publications

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“…On the other hand, it is already known the promoting MJ effect on phenyl-alanine lyase (PAL), which is the first enzyme regulating the bioformation of phenolics through phenylpropanoid pathway. In particular, PAL catalyzes the formation of cinnamic acid from phenylalanine and then cinnamic acid is transformed in naringenin, which is subsequently converted into different flavonoids including phenolic acids [23,24]. In view of our results, the activation of PAL as a result of the preharvest MJ application to olive trees prevailed over ethylene production for Arbequina samples whereas the stimulation of ripening process was clearly predominant over the induction of the polyphenol biosynthesis by PAL activation for Picual olives.…”

Section: Tpc and Dpph Activitymentioning

confidence: 57%

“…An exception was Picual olives picked on day 6 whose content increased from 348.00 to 576.38 mg kg -1 after pre-harvest treatment. Since PAL enzyme has been demonstrated to be activated by MJ [24,26], two possibilities are considered to explain the results on oleuropein content here found. Firstly, MJ might not have any effect on the oleuropein metabolism when pre-harvest applied.…”

Section: Oleuropein and Hydroxytyrosolmentioning

confidence: 95%

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Effect of the Treatment of the Olive Tree (<i>Olea europaea </i>L.) on the Phenolic Content and Antioxidant Properties in Olive Fruits

Blanch¹,

Flores²,

Gómez-Jiménez³

et al. 2018

JFNR

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We here investigate the effects of the application of methyl jasmonate to olive trees on antioxidant composition of olive fruits. Two cultivars (ie, Arbequina and Picual) were evaluated in our study. As a result, the total phenol content increased significantly with the treatment in Arbequina (from 155.89 to 434.22 mg gallic acid kg -1 ) whereas decreases were observed in Picual (from 338.27 to 127.71 mg gallic acid kg -1). Similarly, decreases in phenolic acid content were measured in Arbequina whilst no effect was observed in Picual olives. However, the contents of oleuropein and hydroxytyrosol did not increase with the pre-harvest methyl jasmonate for both Arbequina and Picual. Also for both cultivars the treatment of the olive trees increased the free radical scavenging activity of the olive fruits (IC 50 from 514.36 to 1125.46 µg/mL in Arbequina and from 611.98 to 114.55 µg/mL in Picual). The results here found are deeply discussed.

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Section: Tpc and Dpph Activitymentioning

confidence: 57%

Section: Oleuropein and Hydroxytyrosolmentioning

confidence: 95%

Effect of the Treatment of the Olive Tree (<i>Olea europaea </i>L.) on the Phenolic Content and Antioxidant Properties in Olive Fruits

Blanch¹,

Flores²,

Gómez-Jiménez³

et al. 2018

JFNR

Self Cite

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“…Cabernet sauvignon). Flores et al (2014) also reported a direct correlation between kaempferol concentration and FLS enzymatic activity for strawberry, raspberry, blackberry, redcurrant, and blackcurrant fruits.…”

Section: Discussionmentioning

confidence: 83%

“…Reactions processed without substrates were used as blanks for comparison. Enzyme activity was expressed as pKatal, where one katal is defined as the enzyme activity transforming 1 mol of compound per s (Flores et al, 2014).…”

Section: Enzymatic Activitymentioning

confidence: 99%

“…A recent study by Chacón-Fuentes et al (2015) reported differences in the flavonol concentration present in wild and cultivated plants of U. molinae, with domestication decreasing flavonol production in cultivated plants. Nonetheless, information about FLS activity in berries is still limited (Flores et al, 2014) and currently there is no information about flavonol synthase in U. molinae. We hypothesize that there is lesser FLS activity and kaempferol production in leaves of cultivated U. molinae plants than in wild U. molinae plants.…”

Section: Introductionmentioning

confidence: 99%

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Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process

Chacón-Fuentes

Mutis

Bardehle

et al. 2019

Ciencia e investigación agraria

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M. Chacón-Fuentes, A. Mutis, L. Bardehle, I. Seguel, A. Urzúa, and A. Quiroz. 2019. Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process. Cien. Inv. Agr. 46(1): 30-39. Flavonoid biosynthesis may be affected by plant domestication, with flavonoid production being reduced in proportion to the degree of domestication. In this context, kaempferol (3,4´,5,7-tetrahydroxyflavone) has been identified in the leaves of wild and cultivated Ugni molinae, a berry endemic to Chile. The biosynthetic pathway of kaempferol production begins with naringenin (4´,5,7-trihydroxyflavanone), which is converted to dihydrokaempferol (3,4´,5,7-tetrahydroxyflavanone), catalyzed by flavanone 3ß-hydroxylase (FHT) and then converted to kaempferol by a bifunctional enzyme called flavonol synthase (FLS). Therefore, our study aims to evaluate how FLS activity is affected in murtilla plants that are subjected to the domestication process. Kaempferol was quantified from methanolic extracts of leaf samples collected from both cultivated and wild U. molinae plants using high-performance liquid chromatography, and enzyme extraction was performed to determine FLS activity. The results showed that kaempferol concentration in wild plants from the Soloyo (0.14 μg g -1 ), Mehuín (0.18 μg g -1 ) and Queule (0.25 μg g -1 ) sampling areas was higher than in their cultivated counterparts. Our data are consistent with the FLS activity detected in samples obtained from Manzanal Alto (134.79 pKatal, Soloyo (96.48 pKatal), and Mehuín (119.97 pKatal). These samples also exhibited higher enzymatic activity than their cultivated counterparts. Together, these data suggest that FLS activity is negatively affected by the domestication process.

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Changes in the activity of flavanone 3β‐hydroxylase in blueberry fruit during storage in ozone‐enriched atmosphere

2021

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BACKGROUND: The present study aimed to determine whether the ozonation process affects the flavonoid biosynthesis in highbush blueberry (Vaccinum corymbosum L.) fruit. Flavanone 3⊎-hydroxylase (F3H) was used as a marker of the flavonoid biosynthesis pathway. The activity of F3H, the expression of gene encoding F3H and the antioxidant status in blueberries treated with ozone at a concentration of 15 ppm for 30 min, every 12 h of storage, and maintained at 4 °C for 4 weeks were investigated. RESULTS:The results showed that ozonation process increases the expression of the F3H gene after 1 week of storage, which translates into a higher catalytic capacity of protein, as well as a higher content of flavonoids and total antioxidant potential of ozonated blueberries compared to non-ozonated fruits.CONCLUSION: The present study provides experimental evidence indicating that ozone treatment in proposed process conditions positively affects flavonoid metabolism in highbush blueberry fruit leading to the maintainance of the high quality of the fruit during storage.

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Phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits

Cited by 19 publications

References 24 publications

Effect of the Treatment of the Olive Tree (<i>Olea europaea </i>L.) on the Phenolic Content and Antioxidant Properties in Olive Fruits

Effect of the Treatment of the Olive Tree (<i>Olea europaea </i>L.) on the Phenolic Content and Antioxidant Properties in Olive Fruits

Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process

Changes in the activity of flavanone 3β‐hydroxylase in blueberry fruit during storage in ozone‐enriched atmosphere

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