Phenylalanine induces mango fruit resistance against chilling injuries during storage at suboptimal temperature

Patel, Manish Kumar; Fanyuk, Michal; Feyngenberg, Oleg; Maurer, Dalia; Sela, Noa; Ovadia, Rinat; Sahmir, Michal Oren; Alkan, Noam

doi:10.1016/j.foodchem.2022.134909

Cited by 17 publications

(8 citation statements)

References 70 publications

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“…For the qualitative characterization, a definite weight of DPS was soaked in 90% ethanol to extract its constituents and subsequently filtered to obtain the ethanolic extract, which further subjected to gas chromatography-mass spectrometry (GC‒MS) analysis [ 18 ] and phytochemical analyses. The ethanolic extract (1 µl) was introduced to the GC mass spectrometer (Perkin Elmer model: clarus 580/560S) capillary column (Elite-5MS, 30 m 0.25 mm ID 0.25 µm df) using an autosampler AS3000 coupled with GC in split mode.…”

Section: Methodsmentioning

confidence: 99%

Upregulated synthesis and production of bioactive compounds in Lotus arabicus L. by in vitro feeding with dried powder of date palm seeds

Elsherif,

Safhi,

Khalifa

et al. 2024

BMC Plant Biol

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Background Plants are considered the primary source of many principal bioactive compounds that have been utilized in a wide range of applications including the pharmaceutical and biotechnological industries. Therefore, there is an imperative need to modulate the production of natural bioactive components. The present study aimed to determine the importance of dried and pulverized date palm seeds (DPS) as a natural elicitor for the synthesis of secondary metabolites in Lotus arabicus L. Results The presence of various antioxidant compounds, simple sugars, amino acids, fatty acids and reasonable mineral contents was distinct in the phytochemical characterization of DPS. The major components detected in DPS analysis were the 5-(hydroxymethyl) furfural and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyranone. The induced callus of L. arabicus (seven weeks old) was supplemented with DPS at different concentrations (0, 2, 4, 8 and 10 g/l) in culture media. Treatment with 8 g/l DPS induced the highest antioxidant capacity, ascorbic acid content and secondary metabolites (total phenolics and flavonoids) in the produced callus. Stress biomarkers (hydrogen peroxide and malondialdehyde) were found in the control ranges except at 10 g/l DPS. The expression patterns of key genes involoved in secondary metabolism modulation, such as phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavonol synthase (FLS) and deoxyxylulose phosphate reductoisomerase (DXR), were triggered after DPS treatments. Moreover, the quantitative profiling of phenolic and flavonoid compounds showed that supplementation with DPS, especially at 8 g/l, led to pronounced increases in most of the measured compounds. Conclusion The marked upregulation of eliciting-responsive genes and overproduction of secondary metabolites provide molecular-based evidence for intensifying the principal pathways of phenylpropanoid, flavonoid and terpenoid biosynthesis. Overall, the present in vitro study highlights the stimulating capacity of DPS utilization to improve the bioactive components of L. arabicus at the physiological and molecular levels, enhancing its potential as a medicinal herb.

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Section: Methodsmentioning

confidence: 99%

Upregulated synthesis and production of bioactive compounds in Lotus arabicus L. by in vitro feeding with dried powder of date palm seeds

Elsherif,

Safhi,

Khalifa

et al. 2024

BMC Plant Biol

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“…However, the enzyme activity was significantly diminished under chilling treatment, possibly exceeding the physiological limit of plant cells and affecting the production of related metabolites. When combined with metabolic profiles, increased antioxidants show a correlation with decreased oxidation and lipid peroxidation [22].…”

Section: The Inhibition Effect On Photosynthesis and Antioxidants Und...mentioning

confidence: 99%

“…Cyclocarya paliurus leaves displayed substantial flavonoid accumulation due to reprogramming of transcription factors in flavonoid biosynthesis under salt stress [21]. The phenylalanine-induced phenylpropanoid pathway was found to alleviate chilling injuries in Mangifera indica L. fruit during storage at suboptimal temperatures [22]. Cold-induced changes in sugar accumulation and metabolism were identified in the flower buds of Camellia oleifera through subsequent metabolomics investigations, which revealed the presence of a honey-like mucilaginous substance [12].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomics and Metabolomics Analyses of Avicennia marina and Kandelia obovata under Chilling Stress during Seedling Stage

Wang,

Cheng

2023

IJMS

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One of the most productive ecosystems in the world, mangroves are susceptible to cold stress. However, there is currently insufficient knowledge of the adaptation mechanisms of mangrove plants in response to chilling stress. This study conducted a comparative analysis of transcriptomics and metabolomics to investigate the adaptive responses of Kandelia obovata (chilling-tolerant) and Avicennia marina (chilling-sensitive) to 5 °C. The transcriptomics results revealed that differentially expressed genes (DEGs) were mostly enriched in signal transduction, photosynthesis-related pathways, and phenylpropanoid biosynthesis. The expression pattern of genes involved in photosynthesis-related pathways in A. marina presented a downregulation of most DEGs, which correlated with the decrease in total chlorophyll content. In the susceptible A. marina, all DEGs encoding mitogen-activated protein kinase were upregulated. Phenylpropanoid-related genes were observed to be highly induced in K. obovata. Additionally, several metabolites, such as 4-aminobutyric acid, exhibited higher levels in K. obovata than in A. marina, suggesting that chilling-tolerant varieties regulated more metabolites in response to chilling. The investigation defined the inherent distinctions between K. obovata and A. marina in terms of signal transduction gene expression, as well as phenylpropanoid and flavonoid biosynthesis, during exposure to low temperatures.

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“…For instance, L-glutamic enhanced the resistance of pear fruit to Penicillium expansum and induced blast resistance in rice [ 31 , 32 ]. Phenylalanine treatment boosted mango resistance to both cold injury and anthracnose during storage [ 33 , 34 ]. Additionally, L-cysteine was beneficial to preserve fresh-cut red beets by increasing the contents of gallic acid, caffeic acid, chlorogenic acid, kaempferol and betalains [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Antifungal Activities of L-Methionine and L-Arginine Treatment In Vitro and In Vivo against Botrytis cinerea

Li,

Yu,

Xie

et al. 2024

Microorganisms

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Gray mold caused by Botrytis cinerea is a common postharvest fungal disease in fruit and vegetables. The prevention and treatment of postharvest gray mold has been one of the hot research issues addressed by researchers. This study aimed to investigate the effect of L-methionine and L-arginine on Botrytis cinerea in vitro and on cherry tomato fruit. The results of the in vitro experiment showed that L-methionine and L-arginine had significant inhibitory effects on the mycelial growth and spore germination of Botrytis cinerea, and the inhibitory effects were enhanced with increasing L-methionine or L-arginine concentration. In addition, L-methionine and L-arginine treatment increased the leakage of Botrytis cinerea electrolytes, proteins and nucleic acids. The experiment involving propidium iodide staining and malondialdehyde content assay also confirmed that L-methionine and L-arginine treatment could lead to cell membrane rupture and lipid peroxidation. The results of scanning electron microscopy further verified that the morphology of hyphae was damaged, deformed, dented and wrinkled after treatment with L-methionine or L-arginine. Fruit inoculation experiments displayed that L-methionine and L-arginine treatments significantly inhibited the occurrence and development of gray mold in postharvest cherry tomato. Therefore, treatment with L-methionine or L-arginine might be an effective means to control postharvest gray mold in fruit and vegetables.

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Phenylalanine induces mango fruit resistance against chilling injuries during storage at suboptimal temperature

Cited by 17 publications

References 70 publications

Upregulated synthesis and production of bioactive compounds in Lotus arabicus L. by in vitro feeding with dried powder of date palm seeds

Upregulated synthesis and production of bioactive compounds in Lotus arabicus L. by in vitro feeding with dried powder of date palm seeds

Comparative Transcriptomics and Metabolomics Analyses of Avicennia marina and Kandelia obovata under Chilling Stress during Seedling Stage

Antifungal Activities of L-Methionine and L-Arginine Treatment In Vitro and In Vivo against Botrytis cinerea

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