Phenylpropanoid metabolism enzyme activities and gene expression in postharvest melons inoculated with Alternaria alternata

Bai, Yujia; Feng, Zhongmin; Paerhati, Maerhaba; Wang, Jin

doi:10.1186/s13765-021-00654-x

Cited by 6 publications

(8 citation statements)

References 34 publications

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“…Degradation of PAL activity in ozone-treated chili fruits started to occur only at 20 days (Sachadyn-Król et al, 2016). Bai et al (2021) reported that inoculating Streptomyces spp. into cantaloupes enhanced enzyme activity in cantaloupes promoted lignin synthesis and improved disease resistance in cantaloupes.…”

Section: Discussionmentioning

confidence: 99%

Effect of ozone treatment on phenylpropanoid metabolism in harvested cantaloupes

Ren,

Li,

Dong

et al. 2024

Journal of Food Science

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Phenylpropanoid metabolism plays an important role in cantaloupe ripening and senescence, but the mechanism of ozone regulation on phenylpropanoid metabolism remains unclear. This study investigated how ozone treatment modulates the levels of secondary metabolites associated with phenylpropanoid metabolism, the related enzyme activities, and gene expression in cantaloupe. Treating cantaloupes with 15 mg/m3 of ozone after precooling can help maintain postharvest hardness. This treatment also enhances the production and accumulation of secondary metabolites, such as total phenols, flavonoids, and lignin. These metabolites are essential components of the phenylpropanoid metabolic pathway, activating enzymes like phenylalanine ammonia‐lyase, cinnamate 4‐hydroxylase, 4CL, chalcone synthase, and chalcone isomerase. The results of the transcriptional expression patterns showed that differential gene expression related to phenylpropanoid metabolism in the peel of ozone‐treated cantaloupes was primarily observed during the middle and late storage stages. In contrast, the pulp exhibited significant differential gene expression mainly during the early storage stage. Furthermore, it was observed that the level of gene expression in the peel was generally higher than that in the pulp. The correlation between the relative amount of gene changes in cantaloupe, activity of selected enzymes, and concentration of secondary metabolites could be accompanied by positive regulation of the phenylpropanoid metabolic pathway. Therefore, ozone stress induction positively enhances the biosynthesis of flavonoids in cantaloupes, leading to an increased accumulation of secondary metabolites. Additionally, it also improves the postharvest storage quality of cantaloupes.

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Section: Discussionmentioning

confidence: 99%

Effect of ozone treatment on phenylpropanoid metabolism in harvested cantaloupes

Ren,

Li,

Dong

et al. 2024

Journal of Food Science

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“…Among them, 4CL and C4H are key enzymes in phenylpropanoid metabolic pathway in flavonoid biosynthesis. Some studies have shown that when the expression levels of 4CL and C4H are high in pear and other plants, the content of flavonoid also increases (Bai et al, 2021;Shahivand et al, 2021). But in Lonicera macranthoides, 4CL and C4H negatively regulate the production of flavonoids downstream (Pan et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics explores the potential of flavonoid in non-medicinal parts of Saposhnikovia divaricata (Turcz.) Schischk

et al. 2023

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IntroductionSaposhnikovia divaricata is a traditional Chinese medicine in China, which is widely used in clinic. The root of S. divaricata is often used as medicine, but little research has been done on its other tissues.MethodsIn this study, the contents of root and leaf of S. divaricata were determined by HPLC, the differentially expressed genes were screened by transcriptome sequencing at molecular level, and then verified by network pharmacology.ResultsThe results showed that the content of 4’-O-β-D-glucosyl-5-O-methylvisamminol in the leaves was significantly higher than that in the roots, which was about 3 times higher than that in the roots. In addition, 10 differentially expressed key enzyme genes were screened in plant hormone signal transduction, phenylpropanoid and flavonoid biosynthetic pathways. C4H and CYP98A were up-regulated in root, while F3H was down-regulated in root. They can be used as important candidate genes for the mechanism of quality difference of S. divaricata. Finally, network pharmacological validation showed that 5-O-methylvesamitol plays an important role in the treatment of ulcerative colitis.DiscussionThese findings not only provide insight into flavonoid biosynthesis in S. divaricata associated molecular regulation, but also provide a theoretical basis for the development and utilization of S. divaricata.

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“…Studies have shown that PAL and cinnamic acid coenzyme A ligase (4CL) play a key role in lignin biosynthesis. PAL is the main rate-limiting enzyme that converts L-phenylalanine into trans-cinnamic acid by eliminating ammonia, with its isomers playing a unique but redundant role in plant growth and stress response ( Bai et al., 2021 ). Moreover, 4CL acts as the main branch point enzyme controlling the biosynthesis of downstream molecules, particularly flavonoids and lignans.…”

Section: Discussionmentioning

confidence: 99%

Physiological and transcriptomic analysis reveal the crucial factors in heat stress response of red raspberry ‘Polka’ seedlings

Guo,

Zhang,

Cheng

et al. 2023

Front. Plant Sci.

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With global climate warming, recurring extreme heat and high temperatures irreversibly damage plants. Raspberries, known for their nutritional and medicinal value, are in high demand worldwide. Thus, it is important to study how high-temperature stress (HTS) affects raspberries. The physiological and biochemical responses and molecular genetic mechanisms of raspberry leaves to different HTS treatments were investigated: mild high temperature at 35°C (HT35), severe high temperature at 40°C (HT40), and the control at room temperature of 25°C (CK). The physiological results suggested that leaves in both the 35°C and 40°C treatments showed maximum relative conductivity at 4 d of stress, increasing by 28.54% and 43.36%, respectively, compared to CK. Throughout the stress period (0–4 d), malondialdehyde (MDA) and soluble protein contents of raspberry leaves increased under HT35 and HT40 treatments, while soluble sugar content first decreased and then increased. Catalase (CAT) activity increased, superoxide dismutase (SOD) activity first increased and then decreased, and peroxidase (POD) activity gradually decreased. Photosynthetic and fluorescence responses of raspberry leaves showed the most severe impairment after 4 d of stress. Transcriptomics results revealed significant alterations in 42 HSP family genes, two SOD-related differentially expressed genes (DEGs), 25 POD-related DEGs, three CAT-related DEGs, and 38 photosynthesis-related DEGs under HTS. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that these DEGs were mainly enriched in photosynthesis-antenna proteins, pentose and glucuronide interconversion, phenylpropane biosynthesis, and indole alkaloid biosynthesis. HTS induced excessive ROS accumulation in raspberry leaves, causing oxidative damage in plant cells and subsequently reducing photosynthesis in raspberry leaves. This reduction in photosynthesis, in turn, affects photosynthetic carbon fixation and starch and sucrose metabolism, which, combined with phenol propane biosynthesis, mitigates the HTS-induced damage.

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Phenylpropanoid metabolism enzyme activities and gene expression in postharvest melons inoculated with Alternaria alternata

Cited by 6 publications

References 34 publications

Effect of ozone treatment on phenylpropanoid metabolism in harvested cantaloupes

Effect of ozone treatment on phenylpropanoid metabolism in harvested cantaloupes

Transcriptomics explores the potential of flavonoid in non-medicinal parts of Saposhnikovia divaricata (Turcz.) Schischk

Physiological and transcriptomic analysis reveal the crucial factors in heat stress response of red raspberry ‘Polka’ seedlings

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