Phenylpropanoid production in callus and cell suspension cultures of Buddleja cordata Kunth

Abstract

Plant tissue cultures represent a potential source for producing secondary metabolites. In this work, Buddleja cordata tissue cultures were established in order to produce phenylpropanoids (verbascoside, linarin and hydroxycinnamic acids), as these metabolites are credited with therapeutic properties. Highest callus induction (76.4-84.3%) was obtained in five treatments containing 2,4-Dichlorophenoxyacetic acid (2,4-D: 0.45-9.05 lM) with Kinetin (KIN: 2.32, 4.65 lM), whereas highest root induction (79.6%) corresponded to the a-Naphthaleneacetic acid (9.05 lM) with KIN (2.32 lM) treatment. Verbascoside was the major phenylpropanoid produced in in vitro cultures (root, white and green callus) [66.24-86.26 mg g -1 dry weight (DW)], while linarin and hydroxycinnamic acid production was low (0.95-3.01 mg g -1 DW). Verbascoside and linarin production were improved in cell suspension culture (116 mg g -1 DW and 8.12 mg g -1 DW, respectively).

Establishment of callus and cell suspension culture of Scrophularia striata Boiss.: an in vitro approach for acteoside production

“…The maximum content of acteoside (about 14.25 lg/g cell fresh weight) was observed on the 17th day of cultivation cycle. These results are in agreement with earlier studies (Estrada-Zúñiga et al 2009;Nezbedová et al 1999), that reported acteoside production increased parallel to cell growth, and reached a maximum several days after maximum cell growth. In addition, Ouyang et al (2005) demonstrated at the end of lag phase the cells of Cistanche deserticola started to grow rapidly and the biosynthesis of PeG was activated.…”

“…Production of acteoside has been reported in cell suspension cultures (Chen et al 2007;Estrada-Zúñiga et al 2009;Saimaru and Orihara 2010). Higher acteoside production was reported in suspension cultures than in the callus of Aphelandra sp.…”

“…Acteoside production has been reported in callus cultures (Estrada-Zúñiga et al 2009;Inagaki et al 1991;Shoyama et al 1986). In the present study, trace amount of acteoside was detected in the calli formed on the applied NAA and BA concentrations (data are not shown) except in stem calli subjected to 0.5 mg/l NAA ?…”

“…Plants and cultured cells metabolize foreign compounds in qualitatively similar ways (Hellwig et al 2004), and there are successful examples of tissue cultures for several medicinal plants where an increased content of the bioactive secondary metabolites has been achieved compared to that from wild plants (Estrada-Zúñiga et al 2009). In vitro cultures represent a potential source for producing phenylpropanoids instead of using wild plants (Estrada-Zúñiga et al 2009). Taking into account the increasing demand for these products, plant cell culture provides an attractive alternative source that can overcome the limitations of extracting useful metabolites from limited natural resources.…”

Shoot organogenesis, molecular analysis and secondary metabolite production of micropropagated Rehmannia glutinosa Libosch.

“…In many other plant species callus cultures were reported to be a good source of verbascoside. For example, Estrada-Zúñiga et al (2009) found that callus culture of Buddleja cordata produced 86 mg g -1 DW of verbascoside, compared to 10 and 68 mg g -1 DW in the leaves and roots of the wild plants, respectively. Inagaki et al (1991) reported the production of the compound in callus cultures cultivated on MS medium with different concentrations of an auxin-2,4-D in ten various plant species including of R. glutinosa.…”