Phenytoin accelerates tendon healing in a rat model of Achilles tendon rupture

Hajipour, Babak; Navali, Amir Mohammad; Mohammad, S Ali; Mousavi, Ghafour; Akbari, Mahsa; Miyandoab, T Maleki; Roshangar, Leila; Saleh, B Mohammadi; Kermani, Tanaz A.; Laleh, F Moutab; Ghabili, M

doi:10.4149/bll_2016_107

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…After the 6th week, the remodeling phase begins, and the type I collagen content increases. From approximately week 10, the healing tissue changes from fibrous to scar‐like tissue, and remodeling continues for approximately 1 year 26 . To speed up tendon healing, drugs or methods have been used to influence these stages.…”

Section: Discussionmentioning

confidence: 99%

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Investigation of the effects of pentoxifylline and alpha tocopherol treatment on recovery in rats with Achilles tendon rupture

Toker,

Karaduman,

Arıcan

et al. 2024

Journal Orthopaedic Research

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Although the Achilles tendon is the largest and strongest tendon in the body, healing of the Achilles tendon is the most common injury, and this process is difficult due to poor tendon circulation; moreover, the underlying mechanism has not been fully elucidated. In our study, we aimed to investigate the effects of pentoxifylline and alpha‐tocopherol administered separately or in combination on rats with Achilles tendon injury. Forty‐eight male Wistar rats weighing 230 ± 30 g were used in the study. The rats were randomly divided into eight groups of six animals each. Tendons were evaluated histopathologically and biomechanically. According to the statistical analysis, the vascularity density in the pentoxifylline group on day 14 was significantly greater than that in the other groups (p < 0.05). The collagen arrangement in the pentoxifylline and alpha‐tocopherol groups on day 14 was found to be firmer and smoother than that in the control group (p < 0.05). The collagen arrangement in the pentoxifylline group on day 28 was greater than that in the other groups (p < 0.05). The biomechanical results were significantly greater in all groups (p < 0.05). Pentoxifylline contributed to tendon healing both through neovascularization in the early period and by improving collagen orientation in the late period, while alpha‐tocopherol had a positive effect on collagen orientation in the early period. No beneficial effects were observed when pentoxifylline and alpha‐tocopherol were used together. We believe that further research is needed to understand the effects of this combination therapy on tendon healing.

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Section: Discussionmentioning

confidence: 99%

“…From approximately week 10, the healing tissue changes from fibrous to scar-like tissue, and remodeling continues for approximately 1 year. 26 To speed up tendon healing, drugs or methods have been used to influence these stages. In another animal study of tendon healing, locally applied tranexamic acid accelerated tendon healing.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the effects of pentoxifylline and alpha tocopherol treatment on recovery in rats with Achilles tendon rupture

Toker,

Karaduman,

Arıcan

et al. 2024

Journal Orthopaedic Research

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“…Type III and type IV collagen fibers account for 5% of the total, and mainly exist in immature tendons and injured tendons. Different types of collagen fibers have different functions in tendon tissue [11]. Fibroblasts and tendon cells are the basic functional units of tendon, which synthesize extracellular matrix such as collagen and maintain the metabolism of tendon tissue.…”

Section: Tendon Injurymentioning

confidence: 99%

Cell Biological Characteristics by Fluorescence Microscope and Its Application in Animal Models of Muscle and Tendon Injuries

2022

IJHPM

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With the progress and development of medical science, fluorescence microscope has been widely used in biological research. Muscle and tendon injuries, as common sports injuries, bring a lot of inconvenience to people's daily life and work. At present, the commonly used methods of repairing muscle and tendon injury have certain curative effect, but the treatment cycle is long and the recovery degree is low. In order to better detect the biological characteristics of cells, design muscle and tendon injury model, and explore the treatment of muscle and tendon injury, fluorescence microscope was introduced into the experimental process. Firstly, the muscle satellite cells and tendon stem cells of mice were cultured and passaged. After cryopreservation and resuscitation, the biological characteristics of the cultured cells were detected. The proliferation and cloning of the cells were observed by fluorescence microscope, and the growth curve was drawn. Then the muscle injury model and tendon injury model were established by cardiotoxin and collagenase type I respectively. Paraffin section was made for observation and serum CK index was detected. The experimental results show that fluorescence microscope can effectively observe the biological characteristics of cells and help to establish muscle tendon injury model. It is concluded that muscle satellite cells and tendon stem cells can recover the injury. This provides experimental basis for the clinical application of cell therapy in the treatment of muscle tendon injury.

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Phytosomal Curcumin and Shilajit Decrease Adhesion Bands Post-achilles Tendon Surgery in Animal Model

Asgharzadeh,

Nazari,

Naeimi

et al. 2024

LDDD

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Aim: This study aimed to investigate the protective effects of phytosomal curcumin and/or shilajit in reducing post-surgical tendon adhesion band formation in a rat model. Background: Tendon adhesion is one of the severe complications after tendon surgery which causes limited tendon movement and functional disability. Objective: According to the central role of inflammatory reactions in fibrosis and the formation of tendon adhesions, we investigated the therapeutic effects of phytosomal curcumin and shilajit either alone or in combination on reducing post-surgical tendon adhesion band formation in a rat model. Methods: We randomly divided 36 Wistar male rats into six equal groups. (A) Control group with no surgical incision and no intervention. (B) Sham group with surgical incision, but no adhesion, (C) Positive control group with total surgical transection and adhesion receiving normal saline daily, (D-F) Treatment groups which are the same as group C except that rats were treated with the following drugs. Phytosomal curcumin alone (250 µL/day; orally), shilajit alone (500 mg/kg/day; orally) and a combination of phytosomal curcumin + shilajit. In groups with surgical intervention, the rats' Achilles tendons were cut and repaired with a modified Kessler technique. At 3 weeks, all rats were euthanized. Histological and pathological scoring systems were used to evaluate the protective effects of phytosomal curcumin and/or shilajit in reducing adhesion bands at the site of tendon injuries. Results: Our results revealed that the administration of phytosomal curcumin and/or shilajit remarkably reduced length, density, grading, severity, and thickness of post-surgical adhesion bands. Compared to the untreated control group, the histological changes and inflammatory reactions were significantly attenuated in treated rats. Furthermore, treatment with phytosomal curcumin and/or shilajit inhibited fibrotic responses by alleviating collagen deposition, fibrosis quantity, fibrosis grading, and total fibrosis scores, as visualized by Masson’s trichrome staining. Conclusion: Our findings indicated the anti-inflammatory and anti-fibrotic properties of phytosomal curcumin and/or shilajit supporting their therapeutic potential in preventing post-operative tendon adhesion bands.

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Phenytoin accelerates tendon healing in a rat model of Achilles tendon rupture

Cited by 3 publications

References 14 publications

Investigation of the effects of pentoxifylline and alpha tocopherol treatment on recovery in rats with Achilles tendon rupture

Investigation of the effects of pentoxifylline and alpha tocopherol treatment on recovery in rats with Achilles tendon rupture

Cell Biological Characteristics by Fluorescence Microscope and Its Application in Animal Models of Muscle and Tendon Injuries

Phytosomal Curcumin and Shilajit Decrease Adhesion Bands Post-achilles Tendon Surgery in Animal Model

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