Phillygenin inhibited LPS-induced RAW 264.7 cell inflammation by NF-κB pathway

Zhou, Mengting; Tang, Yunqiu; Liao, Li; Liu, Meichen; Deng, Ying; Zhao, Xingtao; Li, Yunxia

doi:10.1016/j.ejphar.2021.174043

Cited by 36 publications

(22 citation statements)

References 33 publications

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“…Forsythiaside A, phillyrin, and phillygenin have been shown to have significant anti-inflammatory and antioxidant activities in previous studies, but there are few studies on their comparison of effects ( Yuan et al, 2014 ; Ma et al, 2019 ; Du et al, 2020 ; Zhou et al, 2021a ; Tian et al, 2021 ). In this study, correlation analysis demonstrated that phillygenin and phillyrin, which belong to lignans, may be the main anti-inflammatory and antioxidant compounds of FF.…”

Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of Forsythiae Fructus From Different Harvest Seasons and Regions by HPLC/NIR Analysis and Anti-inflammatory and Antioxidant Assays

Dong

et al. 2021

Front. Pharmacol.

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Forsythiae Fructus (FF), the dry fruit of Forsythia suspensa (Thunb.) Vahl, has a long history of use in traditional Chinese Medicine for its heat-clearing and detoxifying properties. It possesses clinical therapeutic effects and biological functions showing efficacy in handling different diseases. To investigate the FF differences in Henan, Shanxi, and Shaanxi in August and October, the surface morphology, mid-infrared and near-infrared spectrums, and HPLC were analyzed. Concurrently, the anti-inflammatory and antioxidant effects on LPS-induced J774A.1 cells were evaluated by western blot and RT-qPCR. The results showed that FF from different Harvest Seasons and Regions are provided with different microstructures and mid-infrared and near-infrared spectrums, and the levels of forsythiaside A and phillyrin of FF from Shanxi in August and phillygenin of FF from Shaanxi in August were the highest. Meanwhile, FF from Shanxi and Shaanxi in August markedly reduced the levels of inflammatory cytokines and mediators (TNF-α, IL-1β, NF-κB, and iNOS) and the protein expression levels of phosphorylated total IKKα/β and nuclear NF-κB. In August, SXFF and SAXFF also promoted the mRNA expression levels of HO-1 and NQO1 and the protein expression levels of HO-1 and nuclear Nrf2 and suppressed the protein expression levels of KEAP1. Spearman correlation analysis showed that phillygenin had a strong correlation with the protein expression on LPS-induced J774A.1 cells. In summary, our results showed that FF from harvest seasons and regions contributed to the distinct differences in microstructure, the mid-infrared and near-infrared spectrums, and compound content. More importantly, FF from Shanxi and Shaanxi in August showed marked anti-inflammatory and antioxidant activities, but with some differences, which may be because of different contents of phillygenin and phillyrin of lignans in FF.

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Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of Forsythiae Fructus From Different Harvest Seasons and Regions by HPLC/NIR Analysis and Anti-inflammatory and Antioxidant Assays

Dong

et al. 2021

Front. Pharmacol.

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“…phillygenin exhibits anti-inflammatory activities (Lee et al, 2011;Lim et al, 2008;Liu et al, 2017;Michalak et al, 2018;Zhou et al, 2021).…”

Section: Ta B L E 1 Effect Of Phillygenin On Inhibition Of Paw Edema ...mentioning

confidence: 99%

“…Since Kitagawa et al (1984) first isolated phillygenin from Forsythia leaves, pharmacological experiments demonstrated that phillygenin can effectively eliminate free radicals (Hung et al, 2012) and showed striking anti‐inflammatory activities. Lim and Zhou reported that phillygenin has anti‐inflammatory activity through inhibiting nitric oxide (NO) and prostaglandin E2 (PGE2) production and nuclear factor‐κB (NF‐κB) activation by RAW 264.7 cells induced by lipopolysaccharide (Lim et al, 2008; Zhou et al, 2021). Liu et al showed that phillygenin reduced the inflammation by inhibiting Akt activation in BEAS‐2B cells (Liu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Lim and Zhou reported that phillygenin has anti-inflammatory activity through inhibiting nitric oxide (NO) and prostaglandin E2 (PGE2) production and nuclear factor-κB (NF-κB) activation by RAW 264.7 cells induced by lipopolysaccharide (Lim et al, 2008;Zhou et al, 2021). Liu et al showed that phillygenin reduced the inflammation by inhibiting Akt activation in BEAS-2B cells (Liu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Phillygenin fromForsythia suspensaleaves exhibits analgesic potential and anti‐inflammatory activity in carrageenan‐induced paw edema in mice

Guo

Tang

Wang

et al. 2022

Journal of Food Biochemistry

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Forsythia suspensa (Thunb.) Vahl (Oleaceae) leaves are valuable sources of phillygenin. This study aimed to isolate phillygenin from F. suspensa leaves and examine its analgesic and anti‐inflammatory effects. Phillygenin was successfully extracted and isolated from F. suspensa leaves after fermentation. Phillygenin significantly reduced the number of writhing induced by acetic acid, prolonged the latency period in the hot plate test, and inhibited the xylene‐induced ear edema and carrageenan‐induced paw edema in mice. IL‐6, TNF‐α, IL‐1β, NO, and PGE2 levels in the carrageenan‐induced paw edema were notably reduced after pretreatment with phillygenin. Phillygenin significantly decreased the iNOS and COX‐2 protein expressions and the IκB‐α and NF‐κB p65 phosphorylation. This study demonstrated that phillygenin is a potential therapeutic candidate for managing pain and inflammation‐mediated disorders. The study contributes to the comprehensive development and utilization of F. suspensa leaves for economic and health care. Practical applications Phillygenin is one of the major active ingredients in Forsythia suspensa. But the content of phillygenin in F. suspensa is very low which limits its application. Phillygenin has potential pharmacological activity and anti‐inflammatory properties. However, the potential effects of phillygenin on analgesic activity have not been clarified. Furthermore, the data on its anti‐inflammatory activity in vivo are relatively limited. This study evaluated the analgesic activity for the first time and the acute anti‐inflammatory effect of phillygenin from F. suspensa leaves by fermentation, which indicated phillygenin is a potential therapeutic candidate for managing pain and inflammation‐mediated disorders.

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“…The membranes were incubated overnight with primary antibodies to identify the target proteins specific for IκB, p65 NF-κB, p38 MAPK and GAPDH at 4 °C. After three 5-min washes with TBS-T solution, the membrane was incubated for 1 h at room temperature with HRP-linked anti-mouse, anti-rabbit, or anti-goat immunoglobulin G secondary antibodies [38,39]. The detection was performed using enhanced chemiluminescence (ECL), ChemiDOC (Davinch-K, Davinch-Invivotm) imaging system.…”

Section: Western Blot Analysismentioning

confidence: 99%

Arteannuin-B and (3-chlorophenyl)-2-spiroisoxazoline derivative exhibit anti-inflammatory effect in LPS-activated RAW 264.7 macrophages and Balb/c mice induced pro-inflammatory responses via down-regulation of NF-κB/P38 MAPK Signalling.

Sawhney

Ahmed

2022

Preprint

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Host inflammatory responses are key to protection against injury; however, persistent inflammation is detrimental and contributes to morbidity and mortality. Herein, we demonstrate the anti-inflammatory role of Arteannuin-B(1) and its novel spirocyclic-2-isoxazoline derivative JR-9 and their side effects in acute inflammatory condition in-vivousing carrageenan-induced paw edema, acetic acid-induced writhing and tail immersion. The results show that spirocyclic-2-isoxazoline derivative has potent anti-inflammatory effect as compared to Arteannuin-B with minimal cell toxicity. In addition, the efficacies of these compounds were also validated by computational and histopathological analysis. Our results show that anti-inflammatory response of JR-9significantly reduce the ability of mouse macrophages to produce NO, TNF-α, and IL-6 following LPS-stimulation.

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Phillygenin inhibited LPS-induced RAW 264.7 cell inflammation by NF-κB pathway

Cited by 36 publications

References 33 publications

Comparative Evaluation of Forsythiae Fructus From Different Harvest Seasons and Regions by HPLC/NIR Analysis and Anti-inflammatory and Antioxidant Assays

Comparative Evaluation of Forsythiae Fructus From Different Harvest Seasons and Regions by HPLC/NIR Analysis and Anti-inflammatory and Antioxidant Assays

Phillygenin fromForsythia suspensaleaves exhibits analgesic potential and anti‐inflammatory activity in carrageenan‐induced paw edema in mice

Arteannuin-B and (3-chlorophenyl)-2-spiroisoxazoline derivative exhibit anti-inflammatory effect in LPS-activated RAW 264.7 macrophages and Balb/c mice induced pro-inflammatory responses via down-regulation of NF-κB/P38 MAPK Signalling.

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