Phlebotomy-induced iron deficiency attenuates the pulmonary toxicity of paraquat in mice

Gohari-Piran, Mahtab; Omidifar, Navid; Mohammadi, Mojdeh; Nili‐Ahmadabadi, Amir

doi:10.1016/j.pestbp.2022.105278

Cited by 9 publications

(10 citation statements)

References 39 publications

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“…[ 52 ] These by‐products are used as diagnostic biomarkers to assess the severity of oxidative damage. [ 53 ] In the current study, the administration of PQ significantly increased the LPO level and decreased the antioxidant capacity of the lung tissue, confirming the findings of Hosseini et al [ 5 ] and Gohari‐Piran et al [ 54 ] It appears that the quaternary nitrogens in the molecular structure of PQ and their interference with the electron transfer cycle of cells are responsible for the formation of free radicals and subsequently lipid and protein oxidation. [ 55 ] Studies indicate that PQ +2 is reduced to PQ + by NADPH cytochrome P450 reductase in the presence of electron donors, [ 56 ] and then the unpaired PQ + electron is fixed by the conjugated double bond in the pyridine ring and quaternary nitrogen in the other ring.…”

Section: Discussionsupporting

confidence: 89%

Chlorogenic acid protective effects on paraquat‐induced pulmonary oxidative damage and fibrosis in rats

Larki‐Harchegani

Fayazbakhsh

Nourian

et al. 2023

J Biochem & Molecular Tox

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Paraquat (PQ) is a widely used herbicide that can cause severe oxidative and fibrotic injuries in lung tissue. Due to the antioxidant and anti-inflammatory properties of chlorogenic acid (CGA), the present study investigated its effects on PQ-induced pulmonary toxicity. To this end, 30 male rats were randomly categorized into five groups of six. Initially, the first and third groups were treated intraperitoneally (IP) with normal saline and CGA (80 mg/kg) for 28 consecutive days, respectively.The second, fourth, and fifth groups were treated with normal saline and 20 and 80 mg/kg of CGA for 28 consecutive days, respectively, and received a single dose of PQ (IP, 20 mg/kg) on Day 7. Then, the animals were anesthetized with ketamine and xylazine, and lung tissue samples were collected for biochemical and histological examinations. The results showed that PQ significantly increased hydroxyproline (HP) and lipid peroxidation (LPO) and decreased the lung tissue antioxidant capacity.In addition, myeloperoxidase (MPO) activity increased significantly, while glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) activity declined substantially. The administration of therapeutic doses of CGA could prevent the oxidative, fibrotic, and inflammatory effects of PQ-induced lung toxicity, and these changes were consistent with histological observations. In conclusion, CGA may improve the antioxidant defense of lung tissue and prevent the spread of inflammation and the development of PQ-induced fibrotic injuries by enhancing antioxidant enzymes and preventing inflammatory cell infiltration.

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Section: Discussionsupporting

confidence: 89%

Chlorogenic acid protective effects on paraquat‐induced pulmonary oxidative damage and fibrosis in rats

Larki‐Harchegani

Fayazbakhsh

Nourian

et al. 2023

J Biochem & Molecular Tox

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“…Based on our previous studies, the toxic dose of PQ was 20 mg/kg [2] and the therapeutic doses of PTX were 25, 50 and 100 mg/kg/ day [12], respectively. This investigation was conducted in two separate phases:…”

Section: Methodsmentioning

confidence: 99%

“…Pulmonary tissue HP content was assessed using the Ehrlich's reagent, as its details outlined by Gohari-Piran et al [2]. Pulmonary MPO activity was assessed following the guidelines provided in the kit obtained from Kiazist Company, Iran.…”

Section: Hydroxyproline Content and Myeloperoxidase Activitymentioning

confidence: 99%

“…In conclusion, our results suggest that the high dose of PTX may ameliorate lung injury by improving the oxidant/antioxidant balance in animals exposed to PQ. [2]. Multiple lines of evidence indicate that the lungs are highly susceptible to the destructive effects of PQ [2,3].…”

Section: Introductionmentioning

confidence: 99%

“…[2]. Multiple lines of evidence indicate that the lungs are highly susceptible to the destructive effects of PQ [2,3]. This herbicide exerts its toxicity primarily through intracellular redox cycle, leading to the generation of reactive oxygen species (ROS) and mitochondrial dysfunction [4].…”

Section: Introductionmentioning

confidence: 99%

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Therapeutic Potential of Pentoxifylline in Paraquat-Induced Pulmonary Toxicity: Role of the Phosphodiesterase Enzymes

Ghasemi,

Mohammadi,

Ghaffari

et al. 2024

Drug Res (Stuttg)

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Pentoxifylline (PTX), a non-selective phosphodiesterase inhibitor, has demonstrated protective effects against lung injury in animal models. Given the significance of pulmonary toxicity resulting from paraquat (PQ) exposure, the present investigation was designed to explore the impact of PTX on PQ-induced pulmonary oxidative impairment in male mice.Following preliminary studies, thirty-six mice were divided into six groups. Group 1 received normal saline, group 2 received a single dose of PQ (20 mg/kg; i.p.), and group 3 received PTX (100 mg/kg/day; i.p.). Additionally, treatment groups 4–6 were received various doses of PTX (25, 50, and 100 mg/kg/day; respectively) one hour after a single dose of PQ. After 72 hours, the animals were sacrificed, and lung tissue was collected.PQ administration caused a significant decrease in hematocrit and an increase in blood potassium levels. Moreover, a notable increase was found in the lipid peroxidation (LPO), nitric oxide (NO), and myeloperoxidase (MPO) levels, along with a notable decrease in total thiol (TTM) and total antioxidant capacity (TAC) contents, catalase (CAT) and superoxide dismutase (SOD) enzymes activity in lung tissue. PTX demonstrated the ability to improve hematocrit levels; enhance SOD activity and TTM content; and decrease MPO activity, LPO and NO levels in PQ-induced pulmonary toxicity. Furthermore, these findings were well-correlated with the observed lung histopathological changes.In conclusion, our results suggest that the high dose of PTX may ameliorate lung injury by improving the oxidant/antioxidant balance in animals exposed to PQ.

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Neuroprotective Effects of Dehydroepiandrosterone and Hericium erinaceus in Scopolamine-induced Alzheimer’s Diseases-like Symptoms in Male Rats

Shirvani,

Nouri,

Sarihi

et al. 2024

Cell Biochem Biophys

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Phlebotomy-induced iron deficiency attenuates the pulmonary toxicity of paraquat in mice

Cited by 9 publications

References 39 publications

Chlorogenic acid protective effects on paraquat‐induced pulmonary oxidative damage and fibrosis in rats

Chlorogenic acid protective effects on paraquat‐induced pulmonary oxidative damage and fibrosis in rats

Therapeutic Potential of Pentoxifylline in Paraquat-Induced Pulmonary Toxicity: Role of the Phosphodiesterase Enzymes

Neuroprotective Effects of Dehydroepiandrosterone and Hericium erinaceus in Scopolamine-induced Alzheimer’s Diseases-like Symptoms in Male Rats

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