Phloem sap proteome studied by iTRAQ provides integrated insight into salinity response mechanisms in cucumber plants

Fan, Huaifu; Xü, Yanli; Du, Changxia; Wu, Xue

doi:10.1016/j.jprot.2015.05.001

Cited by 44 publications

(42 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These include comparative phloem proteomes derived from poplar (Populus spp.) and pumpkin upon wounding stress (Dafoe et al, 2009;Gaupels et al, 2012), rice (Oryza sativa) exposed to plant-hopper insects , salt-stressed cucumber (Fan et al, 2015), melon (Cucumis melo) responding to viral infection (Serra-Soriano et al, 2015), and iron-limited Brassica napus (Gutierrez-Carbonell et al, 2015). A common theme among these proteomes, including this study, is the accumulation of redox-related proteins during stress.…”

Section: Discussion Phloem Proteomicsmentioning

confidence: 90%

Comparative Proteomics Analysis of Arabidopsis Phloem Exudates Collected During the Induction of Systemic Acquired Resistance

et al. 2016

View full text Add to dashboard Cite

ORCID IDs: 0000-0002-5467-7290 (P.C.); 0000-0002-3422-4083 (J.M.-P.).Systemic acquired resistance (SAR) is a plant defense response that provides long-lasting, broad-spectrum pathogen resistance to uninfected systemic leaves following an initial localized infection. In Arabidopsis (Arabidopsis thaliana), local infection with virulent or avirulent strains of Pseudomonas syringae pv tomato generates long-distance SAR signals that travel from locally infected to distant leaves through the phloem to establish SAR. In this study, a proteomics approach was used to identify proteins that accumulate in phloem exudates in response to the induction of SAR. To accomplish this, phloem exudates collected from mock-inoculated or SAR-induced leaves of wild-type Columbia-0 plants were subjected to label-free quantitative liquid chromatography-tandem mass spectrometry proteomics. Comparing mock-and SAR-induced phloem exudate proteomes, 16 proteins were enriched in phloem exudates collected from SAR-induced plants, while 46 proteins were suppressed. SAR-related proteins THIOREDOXIN h3, ACYL-COENZYME A-BINDING PROTEIN6, and PATHOGENESIS-RELATED1 were enriched in phloem exudates of SAR-induced plants, demonstrating the strength of this approach and suggesting a role for these proteins in the phloem during SAR. To identify novel components of SAR, transfer DNA mutants of differentially abundant phloem proteins were assayed for SAR competence. This analysis identified a number of new proteins (m-type thioredoxins, major latex protein-like protein, ULTRAVIOLET-B RESISTANCE8 photoreceptor) that contribute to the SAR response. The Arabidopsis SAR phloem proteome is a valuable resource for understanding SAR long-distance signaling and the dynamic nature of the phloem during plant-pathogen interactions.

show abstract

Section: Discussion Phloem Proteomicsmentioning

confidence: 90%

Comparative Proteomics Analysis of Arabidopsis Phloem Exudates Collected During the Induction of Systemic Acquired Resistance

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Recently, proteomics-based technologies had been broadly employed to identify proteins responsible for salinity tolerance in several crop species such as cotton (Li et al, 2015), cucumber (Fan et al, 2015) and wheat (Jiang et al, 2017), promoting the understanding of changes in cellular activities under salt stress at protein level. For years, two-dimensional gel electrophoresis (2-DE) was one of the widely-used quantitative proteomics methods among biological samples.…”

Section: Introductionmentioning

confidence: 99%

“…Isobaric tags for relative and absolute quantification (iTRAQ) analysis, a gel-free protein quantitative approach containing isotope labeling, has developed into one of the primary proteomic tools. Owing to its technical advantages of high accuracy and sensitivity and more-accurate quantification, iTRAQ had been successfully used in the investigation of DAPS under various abiotic stress conditions including temperature stress (Liu et al, 2014, 2017; Zhang et al, 2017), aluminum stress (Wang et al, 2014) and salt stress (Fan et al, 2015; Li et al, 2015; Xu et al, 2015a; Chen et al, 2016; Jiang et al, 2017), as well as biotic stresses like tobacco mosaic virus (TMV) infection (Wang et al, 2016) and powdery mildew infection (Fu et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.)

Sun

Wang

et al. 2017

Front. Plant Sci.

View full text Add to dashboard Cite

To understand the molecular mechanism underlying salt stress response in radish, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein species abundance under different salt treatments. In total, 851, 706, and 685 differential abundance protein species (DAPS) were identified between CK vs. Na100, CK vs. Na200, and Na100 vs. Na200, respectively. Functional annotation analysis revealed that salt stress elicited complex proteomic alterations in radish roots involved in carbohydrate and energy metabolism, protein metabolism, signal transduction, transcription regulation, stress and defense and transport. Additionally, the expression levels of nine genes encoding DAPS were further verified using RT-qPCR. The integrative analysis of transcriptomic and proteomic data in conjunction with miRNAs was further performed to strengthen the understanding of radish response to salinity. The genes responsible for signal transduction, ROS scavenging and transport activities as well as several key miRNAs including miR171, miR395, and miR398 played crucial roles in salt stress response in radish. Based on these findings, a schematic genetic regulatory network of salt stress response was proposed. This study provided valuable insights into the molecular mechanism underlying salt stress response in radish roots and would facilitate developing effective strategies toward genetically engineered salt-tolerant radish and other root vegetable crops.

show abstract

“…Although the direction of protein change is correct, the iTRAQ assay always leads to fold-change underestimation (for both up- and down-regulation) [32]. Therefore, in this study, we used 1.5-fold and P < 0.05 cut-offs to assess the SA-induced changes in protein abundance, as mentioned in most previous reports [33–36]. Based on these criteria, 381 proteins were defined as differentially expressed proteins (DEPs) upon SA treatment (as listed in S3 and S4 Tables).…”

Section: Resultsmentioning

confidence: 99%

Quantitative Proteomic Profiling of Early and Late Responses to Salicylic Acid in Cucumber Leaves

Dong

Cao

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Salicylic acid (SA) is an important phytohormone that plays vital regulatory roles in plant growth, development, and stress responses. However, studies on the molecular mechanism of SA, especially during the early SA responses, are lagging behind. In this study, we initiated a comprehensive isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomic analysis to explore the early and late SA-responsive proteins in leaves of cucumber (Cucumis sativus L.) seedlings. Upon SA application through the roots, endogenous SA accumulated in cucumber leaves. By assaying the changes in marker gene expression and photosynthetic rate, we collected samples at 12 h and 72 h post treatment (hpt) to profile the early and late SA responsiveness, respectively. The iTRAQ assay followed by tandem mass spectrometry revealed 135 differentially expressed proteins (DEPs) at 12 hpt and 301 DEPs at 72 hpt. The functional categories for these SA-responsive proteins included in a variety of biochemical processes, including photosynthesis, redox homeostasis, carbohydrate and energy metabolism, lipid metabolism, transport, protein folding and modification, proteolysis, cell wall organization, and the secondary phenylpropanoid pathway. Conclusively, based on the abundant changes of these DEPs, together with their putative functions, we proposed a possible SA-responsive protein network. It appears that SA could elicit reactive oxygen species (ROS) production via enhancing the photosynthetic electron transferring, and then confer some growth-promoting and stress-priming effects on cells during the late phase, including enhanced photosynthesis and ROS scavenging, altered carbon metabolic flux for the biosynthesis of amino acids and nucleotides, and cell wall reorganization. Overall, the present iTRAQ assay provides higher proteome coverage and deepened our understanding of the molecular basis of SA-responses.

show abstract

Phloem sap proteome studied by iTRAQ provides integrated insight into salinity response mechanisms in cucumber plants

Cited by 44 publications

References 81 publications

Comparative Proteomics Analysis of Arabidopsis Phloem Exudates Collected During the Induction of Systemic Acquired Resistance

Comparative Proteomics Analysis of Arabidopsis Phloem Exudates Collected During the Induction of Systemic Acquired Resistance

Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.)

Quantitative Proteomic Profiling of Early and Late Responses to Salicylic Acid in Cucumber Leaves

Contact Info

Product

Resources

About