Phloem Transport of the Receptor DWARF14 Protein Is Required for Full Function of Strigolactones

Kameoka, Hiromu; Dun, Elizabeth A.; López-Obando, Mauricio; Brewer, Philip B.; Germain, Alexandre de Saint; Rameau, Catherine; Beveridge, Christine A.; Kyozuka, Junko

doi:10.1104/pp.16.01212

Cited by 36 publications

(36 citation statements)

References 70 publications

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“…DR14 protein could work as an intercellular signaling molecule to fine-tune SL function (Kameoka et al, 2016). Recently, a hypothesis in which SL may indirectly influence seed size has been created.…”

Section: Discussionmentioning

confidence: 99%

De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

et al. 2017

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Yellow lupine (Lupinus luteus L., Taper c.), a member of the legume family (Fabaceae L.), has an enormous practical importance. Its excessive flower and pod abscission represents an economic drawback, as proper flower and seed formation and development is crucial for the plant's productivity. Generative organ detachment takes place at the basis of the pedicels, within a specialized group of cells collectively known as the abscission zone (AZ). During plant growth these cells become competent to respond to specific signals that trigger separation and lead to the abolition of cell wall adhesion. Little is known about the molecular network controlling the yellow lupine organ abscission. The aim of our study was to establish the divergences and similarities in transcriptional networks in the pods, flowers and flower pedicels abscised or maintained on the plant, and to identify genes playing key roles in generative organ abscission in yellow lupine. Based on de novo transcriptome assembly, we identified 166,473 unigenes representing 219,514 assembled unique transcripts from flowers, flower pedicels and pods undergoing abscission and from control organs. Comparison of the cDNA libraries from dropped and control organs helped in identifying 1,343, 2,933 and 1,491 differentially expressed genes (DEGs) in the flowers, flower pedicels and pods, respectively. In DEG analyses, we focused on genes involved in phytohormonal regulation, cell wall functioning and metabolic pathways. Our results indicate that auxin, ethylene and gibberellins are some of the main factors engaged in generative organ abscission. Identified 28 DEGs common for all library comparisons are involved in cell wall functioning, protein metabolism, water homeostasis and stress response. Interestingly, among the common DEGs we also found an miR169 precursor, which is the first evidence of micro RNA engaged in abscission. A KEGG pathway enrichment analysis revealed that the identified DEGs were predominantly involved in carbohydrate and amino acid metabolism, but some other pathways were also targeted. This study represents the first comprehensive transcriptome-based characterization of organ abscission in L. luteus and provides a valuable data source not only for understanding the abscission signaling pathway in yellow lupine, but also for further research aimed at improving crop yields.

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Section: Discussionmentioning

confidence: 99%

De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

et al. 2017

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“…This is particularly important for SL because shoot branching is a complicated process involving local and systemic decisions. SL signaling genes are expressed in the buds as well as in vascular bundles, consistent with the two modes of SL function in which SL regulates the PATS and downstream transcription cascades (Stirnberg et al ., ; Zhou et al ., ; Chevalier et al ., ; Soundappan et al ., ; Kameoka et al ., ; Liang et al ., ). However, more precise identification of the site of SL function is required to better understand the mechanisms by which SL inhibits bud outgrowth.…”

Section: Introductionmentioning

confidence: 99%

Developmental analysis of the early steps in strigolactone‐mediated axillary bud dormancy in rice

et al. 2019

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SummaryBy contrast with rapid progress in understanding the mechanisms of biosynthesis and signaling of strigolactone (SL), mechanisms by which SL inhibits axillary bud outgrowth are less well understood. We established a rice (Oryza sativa L.) hydroponic culture system to observe axillary buds at the critical point when the buds enter the dormant state. In situ hybridization analysis indicated that cell division stops in the leaf primordia of the buds entering dormancy. We compared transcriptomes in the axillary buds isolated by laser capture microdissection before and after entering the dormant state and identified genes that are specifically upregulated or downregulated in dormant buds respectively, in SL‐mediated axillary bud dormancy. Typically, cell cycle genes and ribosomal genes are included among the active genes while abscisic acid (ABA)‐inducible genes are among the dormant genes. Application of ABA to the hydroponic culture suppressed the growth of axillary buds of SL mutants to the same level as wild‐type (WT) buds. Tiller number was decreased in the transgenic lines overexpressing OsNCED1, the gene that encodes ABA biosynthesis enzyme. These results indicated that the main site of SL function is the leaf primordia in the axillary bud and that ABA is involved in SL‐mediated axillary bud dormancy.

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“…A recent study indicated that DWARF14 (D14), a receptor of strigolactone (SL) hormone in rice, is transported long distances through phloem and then moves into axillary meristems (AMs) to regulate the function of SLs in the development of AMs [40]. D14 mRNA accumulates in vascular bundles and leaf primordia, whereas natively expressed GFP-fused D14 protein (D14-GFP) can be found in AMs, suggesting that it is mobile.…”

Section: Macromolecular Trafficking Through Pd In the Sammentioning

confidence: 99%

Plasmodesmata-Mediated Cell-to-Cell Communication in the Shoot Apical Meristem: How Stem Cells Talk

Kitagawa

Jackson

2017

Plants

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Positional information is crucial for the determination of plant cell fates, and it is established based on coordinated cell-to-cell communication, which in turn is essential for plant growth and development. Plants have evolved a unique communication pathway, with tiny channels called plasmodesmata (PD) spanning the cell wall. PD interconnect most cells in the plant and generate a cytoplasmic continuum, to mediate short- and long-distance trafficking of various molecules. Cell-to-cell communication through PD plays a role in transmitting positional signals, however, the regulatory mechanisms of PD-mediated trafficking are still largely unknown. The induction and maintenance of stem cells in the shoot apical meristem (SAM) depends on PD-mediated cell-to-cell communication, hence, it is an optimal model for dissecting the regulatory mechanisms of PD-mediated cell-to-cell communication and its function in specifying cell fates. In this review, we summarize recent knowledge of PD-mediated cell-to-cell communication in the SAM, and discuss mechanisms underlying molecular trafficking through PD and its role in plant development.

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Phloem Transport of the Receptor DWARF14 Protein Is Required for Full Function of Strigolactones

Cited by 36 publications

References 70 publications

De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

Developmental analysis of the early steps in strigolactone‐mediated axillary bud dormancy in rice

Plasmodesmata-Mediated Cell-to-Cell Communication in the Shoot Apical Meristem: How Stem Cells Talk

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