Phloretin inhibits biofilm formation by affecting quorum sensing under different temperature

Li, Wei; Shi, Chang Zheng; Luo, Cang Xue; Hu, Ching Yuan; Meng, Yong Hong

doi:10.1016/j.lwt.2020.109668

Cited by 31 publications

(15 citation statements)

References 32 publications

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“…In the presence of nonlethal phloretin concentrations, biofilm formation in Pb1692 was significantly reduced, already at 0.2 mM phloretin, a concentration that had no effect on growth. A dose-dependent reduction in biofilm formation in response to phloretin was also reported in E. coli O157:H7, where it reduces cell aggregation and decreases biofilm adhesion (Lee et al, 2011;Wei et al, 2020). That inhibitory effect has been related to interference with autoinducer (AI-2) QS and signaling (Lee et al, 2011).…”

Section: Discussionmentioning

confidence: 78%

Phloretin, an Apple Phytoalexin, Affects the Virulence and Fitness of Pectobacterium brasiliense by Interfering With Quorum-Sensing

et al. 2021

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The effects of phloretin a phytoalexin from apple, was tested on Pectobacterium brasiliense (Pb1692), an emerging soft-rot pathogen of potato. Exposure of Pb1692 to 0.2 mM phloretin a concentration that does not affect growth, or to 0.4 mM a 50% growth inhibiting concentration (50% MIC), reduced motility, biofilm formation, secretion of plant cell wall-degrading enzymes, production of acyl–homoserine lactone (AHL) signaling molecules and infection, phenotypes that are associated with bacterial population density-dependent system known as quorum sensing (QS). To analyze the effect of growth inhibition on QS, the activity of ciprofloxacin, an antibiotic that impairs cell division, was compared to that of phloretin at 50% MIC. Unlike phloretin, the antibiotic hardly affected the tested phenotypes. The use of DH5α, a QS-negative Escherichia coli strain, transformed with an AHL synthase (ExpI) from Pb1692, allowed to validate direct inhibition of AHL production by phloretin, as demonstrated by two biosensor strains, Chromobacterium violaceaum (CV026) and E. coli (pSB401). Expression analysis of virulence-related genes revealed downregulation of QS-regulated genes (expI, expR, luxS, rsmB), plant cell wall degrading enzymes genes (pel, peh and prt) and motility genes (motA, fim, fliA, flhC and flhD) following exposure to both phloretin concentrations. The results support the inhibition of ExpI activity by phloretin. Docking simulations were used to predict the molecular associations between phloretin and the active site of ExpI, to suggest a likely mode of action for the compound’s inhibition of virulence.

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Section: Discussionmentioning

confidence: 78%

Phloretin, an Apple Phytoalexin, Affects the Virulence and Fitness of Pectobacterium brasiliense by Interfering With Quorum-Sensing

et al. 2021

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“…The thickness of the biofilm was lessened by 2 µm at a concentration of 20 µg/mL. The mode of biofilm inhibition study revealed the role of phloretin reducing the QS related gene agr by 50% with 20 µg/mL phloretin [200,201].…”

Section: Natural Compoundsmentioning

confidence: 95%

Development of Antibiofilm Therapeutics Strategies to Overcome Antimicrobial Drug Resistance

et al. 2022

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A biofilm is a community of stable microorganisms encapsulated in an extracellular matrix produced by themselves. Many types of microorganisms that are found on living hosts or in the environment can form biofilms. These include pathogenic bacteria that can serve as a reservoir for persistent infections, and are culpable for leading to a broad spectrum of chronic illnesses and emergence of antibiotic resistance making them difficult to be treated. The absence of biofilm-targeting antibiotics in the drug discovery pipeline indicates an unmet opportunity for designing new biofilm inhibitors as antimicrobial agents using various strategies and targeting distinct stages of biofilm formation. The strategies available to control biofilm formation include targeting the enzymes and proteins specific to the microorganism and those involved in the adhesion pathways leading to formation of resistant biofilms. This review primarily focuses on the recent strategies and advances responsible for identifying a myriad of antibiofilm agents and their mechanism of biofilm inhibition, including extracellular polymeric substance synthesis inhibitors, adhesion inhibitors, quorum sensing inhibitors, efflux pump inhibitors, and cyclic diguanylate inhibitors. Furthermore, we present the structure–activity relationships (SAR) of these agents, including recently discovered biofilm inhibitors, nature-derived bioactive scaffolds, synthetic small molecules, antimicrobial peptides, bioactive compounds isolated from fungi, non-proteinogenic amino acids and antibiotics. We hope to fuel interest and focus research efforts on the development of agents targeting the uniquely complex, physical and chemical heterogeneous biofilms through a multipronged approach and combinatorial therapeutics for a more effective control and management of biofilms across diseases.

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“…Biofilms are sessile microbial communities in which bacterial cells are encased in a matrix composed of self-produced extracellular polymeric substances (EPS), mainly composed of proteins, polysaccharides, lipids, and extracellular DNA (Olsen 2015;Xie et al 2017). When biofilm-associated cells are exposed to unfavourable environments, such as nutritional deficiency, antibacterial agents, high salinity, or low water availability, the extracellular polymeric substances of biofilm can act as a protective barrier and provide an appropriate growth surrounding for pathogenic bacteria (Pang et al 2019;Wei et al 2020). Once the biofilm is formed, it is difficult to remove because its special spatial structure shows stronger environmental adaptability (Oloketuyi and Khan 2017).…”

Section: Introductionmentioning

confidence: 99%

“…2019; Wei et al . 2020). Once the biofilm is formed, it is difficult to remove because its special spatial structure shows stronger environmental adaptability (Oloketuyi and Khan 2017).…”

Section: Introductionmentioning

confidence: 99%

The elimination effects of lavender essential oil on Listeria monocytogenes biofilms developed at different temperatures and the induction of VBNC state

Han

Chen

Zhang

et al. 2022

Letters in Applied Microbiology

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Significance and Impact of this Study:The biofilm and viable but non-culturable state (VBNC) of Listeria monocytogenes are regarded as potential harms to public health. It is of great significance to apply lavender essential oil (LEO) as an alternative of disinfectant and antibiotics to control the pathogenicity and drug resistance of pathogens enhanced by biofilms. This work mainly evaluated the elimination effects of LEO on L. monocytogenes biofilms formed at 32°C (warm biofilm) and 10°C (cold biofilm). The results presented that LEO exhibited effective eradicating effects on L. monocytogenes biofilms formed at different temperatures, significantly reduced the VBNC cell number in mature biofilms and promoted the dispersion of biofilm cells into plankton. Our findings support that LEO has the potential to be applied as an ideal antibiofilm agent to control the contamination and infection of L. monocytogenes.

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Phloretin inhibits biofilm formation by affecting quorum sensing under different temperature

Cited by 31 publications

References 32 publications

Phloretin, an Apple Phytoalexin, Affects the Virulence and Fitness of Pectobacterium brasiliense by Interfering With Quorum-Sensing

Phloretin, an Apple Phytoalexin, Affects the Virulence and Fitness of Pectobacterium brasiliense by Interfering With Quorum-Sensing

Development of Antibiofilm Therapeutics Strategies to Overcome Antimicrobial Drug Resistance

The elimination effects of lavender essential oil on Listeria monocytogenes biofilms developed at different temperatures and the induction of VBNC state

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