Phlorizin Administration Does Not Attenuate Hypophagia Induced by Intraruminal Propionate Infusion in Lactating Dairy Cattle

Abstract

Infusion data from ruminants has shown that propionate stimulates satiety and decreases meal size, possibly because of increased propionate oxidation in the liver. In this experiment, phlorizin was used to increase glucose demand, which was expected to decrease propionate oxidation and attenuate the decrease in dry matter intake (DMI) caused by propionate infusion. Twelve multiparous, ruminally-cannulated Holstein cows (49+/-33 d in milk, 40+/-7 kg/d milk; mean+/-SD) were randomly assigned to square and treatment sequence in a replicated 4x4 Latin square experiment with a 2x2 factorial arrangement of treatments. Treatments were subcutaneous injection of phlorizin or propylene glycol in combination with intraruminal infusion of either Na acetate or Na propionate. Following a 7-d adaptation period, phlorizin (4 g/d) and control injections were administered every 6 h for 7 d. During the final 2 d of injections, Na acetate or Na propionate solutions (1 mol/L, pH 6.0) were infused continuously at the rate of 0.80 L/h. Feeding behavior data were collected during the final 2 d of treatment. Phlorizin caused urinary excretion of 400+/-40 g glucose/d across infusion treatments. Phlorizin tended to increase plasma free fatty acid and beta-hydroxybutyrate concentrations to a greater extent with Na acetate compared to Na propionate infusion (both interactions P<0.15). Phlorizin decreased and Na propionate increased plasma insulin and glucose concentrations. Infusion of Na propionate decreased DMI (18.4 vs. 21.1+/-1.4 kg/d, P<0.001) through an increase in intermeal interval (89.2 vs. 77.3+/-6.6 min, P=0.03), resulting in fewer meals per day (11.6 vs. 13.7+/-0.7, P<0.001). Phlorizin did not alter DMI (P=0.39) or measures of feeding behavior, nor were there interactions with infusion type. Increasing glucose demand does not limit the extent to which propionate decreases DMI in lactating dairy cows.

The effect of calcium propionate supplementation on performance, meat quality, and mRNA expression of finishing steers fed a high-concentrate diet

“…Similarly, Villalba et al (1996) found that supplementing calcium propionate at 8.3 g · day -1 increased the feed intake of lambs fed poor quality straw, whereas supplementation at 16.6 g · day -1 decreased feed intake. Nevertheless, some authors also observed that calcium propionate supplementation depressed feed intake (Rigout et al, 2003;Bradford and Allen, 2007). Therefore, we can suppose that inconsistent effects of calcium propionate supplementation on dry matter intake may depend on the nutrition level of basic diets and the doses of calcium propionate supplementation.…”

Effects of Dietary Calcium Propionate Supplementation on Hypothalamic Neuropeptide Messenger RNA Expression and Growth Performance in Finishing Rambouillet Lambs

“…Likewise, Lee-Rangel et al [ 4 ] and Mendoza-Martinez et al [ 1 ] found no hypophagic effects of calcium propionate at a daily intake of 64.3 and 130.27 mmol of propionate in finishing lambs, respectively. Hypophagic effects of propionate have been found more frequently when propionate was infused into rumen, portal, or mesenteric veins [ 24 , 25 , 26 ]. However, when propionate was included in diets, its hypophagic effect has been limited [ 27 ] or not shown [ 28 , 29 , 30 ].…”

Propionate Promotes Fatty Acid Oxidation through the Up-Regulation of Peroxisome Proliferator-Activated Receptor &alpha; in Intestinal Epithelial Cells

“…The potential effects of propionate on physiological and pathophysiological events have been underestimated (6). Reportedly, propionate lowers fatty acids content in plasma and reduces food intake (7)(8)(9)(10). However, the detailed mechanisms underlying the propionate-mediated lipid metabolism action remain poorly defined.…”