Phoenixin-14 reduces the frequency of interictal-like events in mice brain slices

Kalkan, Ömer Faruk; Şahin, Zafer; ÖZTÜRK, Hilal; Keser, Hatice; Aydın-Abidin, Selcen; Abidin, İsmail

doi:10.1007/s00221-021-06179-5

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…Apart from stroke models, phoenixin-14 was recently shown to be a potential therapeutic target for epilepsy, since it reduced interictal-like spikes in an epilepsy model in murine brain slices ( Kalkan et al, 2021 ). This is especially interesting given the fact that a deletion of phoenixin’s precursor molecule was shown to induce epileptiform seizures ( Kinton et al, 2002 ).…”

Section: State Of Knowledgementioning

confidence: 99%

Current state of phoenixin—the implications of the pleiotropic peptide in stress and its potential as a therapeutic target

Friedrich

Stengel²

2023

Front. Pharmacol.

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Phoenixin is a pleiotropic peptide, whose known functions have broadened significantly over the last decade. Initially first described as a reproductive peptide in 2013, phoenixin is now recognized as being implicated in hypertension, neuroinflammation, pruritus, food intake, anxiety as well as stress. Due to its wide field of involvement, an interaction with physiological as well as psychological control loops has been speculated. It has shown to be both able to actively reduce anxiety as well as being influenced by external stressors. Initial rodent models have shown that central administration of phoenixin alters the behavior of the subjects when confronted with stress-inducing situations, proposing an interaction with the perception and processing of stress and anxiety. Although the research on phoenixin is still in its infancy, there are several promising insights into its functionality, which might prove to be of value in the pharmacological treatment of several psychiatric and psychosomatic illnesses such as anorexia nervosa, post-traumatic stress disorder as well as the increasingly prevalent stress-related illnesses of burnout and depression. In this review, we aim to provide an overview of the current state of knowledge of phoenixin, its interactions with physiological processes as well as focus on the recent developments in stress response and the possible novel treatment options this might entail.

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Section: State Of Knowledgementioning

confidence: 99%

Current state of phoenixin—the implications of the pleiotropic peptide in stress and its potential as a therapeutic target

Friedrich

Stengel²

2023

Front. Pharmacol.

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“…In addition, phoenixin-20 significantly inhibits the NADPH oxidase 4 (NOX-4) and reactive oxygen species (ROS) induced by LPS ( Zeng, et al, 2020 ). Electrophysiological recordings from brain slices demonstrated that phoenixin-14 reduced the interictal-like events in the entorhinal cortex and hippocampus ( Kalkan et al, 2021 ), suggesting a potential role of phoenixin-14 in epileptiform activity. Recent study demonstrated that inflammatory stress induced by LPS up-regulated phoenixin expression and activity in the specific brain nuclei of SD Rats, indicating that several stressors could activate phoenixin signaling ( Friedrich et al, 2022 ).…”

Section: Function Of Phoenixinmentioning

confidence: 99%

Regulation and physiological functions of phoenixin

Liu

Zhao

et al. 2022

Front. Mol. Biosci.

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Phoenixin is a newly discovered neuropeptide generated from small integral membrane protein 20. Phoenixin is a ligand for the G protein-coupled receptor 173 (GPR173) and has been detected in central and peripheral tissues of human, rats, mice, bovine, and zebrafish. It was initially involved in regulating reproductive function by stimulating the luteinizing hormone release from pituitary cells by increasing the level of gonadotropin-releasing hormone. Recently, many functions of phoenixin have been generalized, including regulation of food intake, memory, Alzheimer’s disease, anxiety, inflammation, neuronal and microglial activity, energy metabolism and body fluid balance, cardiovascular function, and endocrine activity. In addition, the interaction between phoenixin and nesfatin-1 have been revealed. The present article summarized the latest research progress on physiological function of phoenixin, suggesting that it is a potential target for novel drug development and clinical application.

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Phoenixin‐14 maintains the contractile type of vascular smooth muscle cells in cerebral aneurysms rats

Ling,

Yang,

Zhang

et al. 2024

J Biochem & Molecular Tox

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The rupture of intracranial aneurysm (IA) is the primary reason contributing to the occurrence of life‐threatening subarachnoid hemorrhages. The oxidative stress‐induced phenotypic transformation from the contractile phenotype to the synthetic phenotype of vascular smooth muscle cells (VSMCs) plays a pivotal role in IA formation and rupture. Our study aimed to figure out the role of phoenixin‐14 in VSMC phenotypic switching during the pathogenesis of IA by using both cellular and animal models. Primary rat VSMCs were isolated from the Willis circle of male Sprague‐Dawley rats. VSMCs were stimulated by hydrogen peroxide (H2O2) to establish a cell oxidative damage model. After pretreatment with phoenixin‐14 and exposure to H2O2, VSMC viability, migration, and invasion were examined through cell counting kit‐8 (CCK‐8), wound healing, and Transwell assays. Intracellular reactive oxygen species (ROS) production in VSMCs was evaluated by using 2′,7′‐Dichlorofluorescin diacetate (DCFH‐DA) fluorescence probes and flow cytometry. Rat IA models were established by ligation of the left common carotid arteries and posterior branches of both renal arteries. The histopathological changes of rat intracranial blood vessels were observed through hematoxylin and eosin staining. The levels of contractile phenotype markers (alpha‐smooth muscle actin [α‐SMA] and smooth muscle 22 alpha [SM22α]) in VSMCs and rat arterial rings were determined through real‐time quantitative polymerase chain reaction (RT‐qPCR) and western blot analysis. Our results showed that H2O2 stimulated the production of intracellular ROS and induced oxidative stress in VSMCs, while phoenixin‐14 pretreatment attenuated intracellular ROS levels in H2O2‐exposed VSMCs. H2O2 exposure promoted VSMC migration and invasion, which, however, was reversed by phoenixin‐14 pretreatment. Besides, phoenixin‐14 administration inhibited IA formation and rupture in rat models. The decrease in α‐SMA and SM22α levels in H2O2‐exposed VSMCs and IA rat models was antagonized by phoenixin‐14. Collectively, phoenixin‐14 ameliorates the progression of IA through preventing the loss of the contractile phenotype of VSMCs.

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Phoenixin-14 reduces the frequency of interictal-like events in mice brain slices

Cited by 4 publications

References 33 publications

Current state of phoenixin—the implications of the pleiotropic peptide in stress and its potential as a therapeutic target

Current state of phoenixin—the implications of the pleiotropic peptide in stress and its potential as a therapeutic target

Regulation and physiological functions of phoenixin

Phoenixin‐14 maintains the contractile type of vascular smooth muscle cells in cerebral aneurysms rats

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