Phoenixin-20 suppresses lipopolysaccharide-induced inflammation in dental pulp cells

Sun, Guohui; Ren, Qihui; Bai, Li; Zhang, Lin

doi:10.1016/j.cbi.2020.108971

Cited by 29 publications

(31 citation statements)

References 26 publications

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“…In our research, we found that the addition of phoenixin 20 signi cantly alleviated the increase in the levels of IL-8, IL-1β, and MCP-1 induced by ox-LDL. Consistently, a recent study demonstrates that the activation of GPR173 by phoenixin-20 exerts a robust anti-in ammatory effect in dental pulp cells by reducing the expression of proin ammatory cytokines, cell adhesion molecules, MMP-2, and MMP-9 [17]. NOX-4, which is present in mitochondria and localizes to membranes, has been shown to be a major source of ROS in endothelial cells.…”

Section: Discussionsupporting

confidence: 62%

Phoenixin-20 Prevents ox-LDL-Induced Attachment of Monocytes to Human Aortic Endothelial Cells (HAECs): A Protective Implication in Atherosclerosis

Wei

Lin

Zhang

et al. 2021

ACS Chem. Neurosci.

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Background: The cause of atherosclerosis is not known, and therefore the current treatment options are limited. In the present study, we aimed to investigate the effects of Phoenixin 20 and its receptor G protein-coupled receptor 173 (GPR173) against ox-LDL-induced endothelial dysfunction.Materials and Methods: Human aortic endothelial cells (HAECs) were treated with 10 μg/ml ox-LDL in the presence or absence of phoenixin 20. Gene expression of GPR173, ICAM-1, VCAM-1, IL-1β, IL-8, MCP-1, and NOX-4 were measured by real time PCR. Protein expression was assayed by western blot analysis. Secretions of pro-in ammatory cytokines were measured by ELISA. The attachment of THP-1 monocytes to HAECs was detected using calcein-AM staining. Transcriptional activity of NF-κB was measured using dual-luciferase reporter assay.Results: Our ndings indicate that ox-LDL signi cantly lowered the expression of GPR173 in HAECs and triggered an increase in ROS, NOX-4, and proin ammatory cytokine expression. Importantly, we demonstrate that agonism of GPR173 using phoenixin 20 signi cantly ameliorated these harmful effects of ox-LDL. We also show that agonism of GPR173 can prevent the attachment of monocytes to endothelial cells, which is an important therapeutic approach to prevent atherogenesis. Conclusion:Here, for the rst time to our knowledge, we provide a basis for future research on the role of GPR173 as a new potential treatment against atherosclerosis.

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Section: Discussionsupporting

confidence: 62%

Phoenixin-20 Prevents ox-LDL-Induced Attachment of Monocytes to Human Aortic Endothelial Cells (HAECs): A Protective Implication in Atherosclerosis

Wei

Lin

Zhang

et al. 2021

ACS Chem. Neurosci.

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show abstract

“…Since integrins and E-cadherin play a pivotal role in the inter-relation of epithelial cells with the extracellular matrix [17,18], we focused on these parameters to investigate cell barrier function. Inflammatory cytokines, such as interleukin (IL)-8, a strong factor associated with chemotaxis, and IL-6 and intercellular adhesion molecule 1 (ICAM-1) [19,20], both associated with inflammatory conditions, were also investigated in this study.…”

Section: Introductionmentioning

confidence: 99%

Behaviour of Human Oral Epithelial Cells Grown on Invisalign® SmartTrack® Material

et al. 2020

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Invisalign aligners have been widely used to correct malocclusions, but their effect on oral cells is poorly known. Previous research evaluated the impact of aligners’ eluates on various cells, but the cell behavior in direct contact with aligners is not yet studied. In the present study, we seeded oral epithelial cells (cell line Ca9-22) directly on Invisalign SmartTrack material. This material is composed of polyurethane and co-polyester and exhibit better mechanical characteristics compared to the predecessor. Cell morphology and behavior were investigated by scanning electron microscopy and an optical cell moves analyzer. The effect of aligners on cell proliferation/viability was assessed by cell-counting kit (CCK)-8 and 3,4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) assay and live/dead staining. The expression of inflammatory markers and proteins involved in epithelial barrier function was measured by qPCR. Cells formed cluster-like structures on aligners. The proliferation/viability of cells growing on aligners was significantly lower (p < 0.05) compared to those growing on tissue culture plastic (TCP). Live/dead staining revealed a rare occurrence of dead cells on aligners. The gene expression level of all inflammatory markers in cells grown on aligners’ surfaces was significantly increased (p < 0.05) compared to cells grown on TCP after two days. Gene expression levels of the proteins involved in barrier function significantly increased (p < 0.05) on aligners’ surfaces after two and seven days of culture. Aligners’ material exhibits no cytotoxic effect on oral epithelial cells, but alters their behavior and the expression of proteins involved in the inflammatory response, and barrier function. The clinical relevance of these effects has still to be established.

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“…Among them, ICAM1 (a well-known gene involved in viral infection and cardiovascular disease) expression has been demonstrated to be consistent with IL-6 expression in mouse macrophages [28] , [29] . Some treatments, such as Phoenix 20 and soluble matrine 2, can induce the co-expression of ICAM1 and IL-6 [30] , [31] . In human astrocytes, IL-6 can upregulate SBNO2 expression [32] .…”

Section: Discussionmentioning

confidence: 99%

Meta-analysis of transcriptome datasets: An alternative method to study IL-6 regulation in coronavirus disease 2019

Liu¹,

Lin²,

Ao³

et al. 2021

Computational and Structural Biotechnology Journal

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Phoenixin-20 suppresses lipopolysaccharide-induced inflammation in dental pulp cells

Cited by 29 publications

References 26 publications

Phoenixin-20 Prevents ox-LDL-Induced Attachment of Monocytes to Human Aortic Endothelial Cells (HAECs): A Protective Implication in Atherosclerosis

Phoenixin-20 Prevents ox-LDL-Induced Attachment of Monocytes to Human Aortic Endothelial Cells (HAECs): A Protective Implication in Atherosclerosis

Behaviour of Human Oral Epithelial Cells Grown on Invisalign® SmartTrack® Material

Meta-analysis of transcriptome datasets: An alternative method to study IL-6 regulation in coronavirus disease 2019

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