Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

Abstract

Pholiota nameko, a type of edible and medicinal fungus, is currently grown extensively for food and traditional medicine in China and Japan. It possesses various biological activities, such as anti-inflammatory, anti-hyperlipidemia and antitumor activities. However, P. nameko has rarely been discussed in the field of dermatology; identifying its biological activities could be beneficial in development of a new natural ingredient used in wound care. To evaluate its in vitro wound healing activities, the present study assessed the antioxidant and anti-collagenase activities of P. nameko polysaccharides (PNPs) prepared through fractional precipitation (40%, 60% and 80% (v/v)); the assessments were conducted using reducing power, hydroxyl radical scavenging activity, dichloro-dihydro-fluorescein diacetate and collagenase activity assays. The ability of PNPs to facilitate L929 fibroblast cell proliferation and migration was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and scratch assays. The findings indicated that, among all fractions, PNP-80 showed the best antioxidant and anti-collagenase activity, as measured by their reducing power (IC50 of PNP-80 was 2.43 ± 0.17 mg/mL), the hydroxyl radical scavenging (IC50 of PNP-80 was 2.74 ± 0.11 mg/mL) and collagenase activity assay, and significantly reduced cellular ROS content, compared with that of H2O2-induced L929 cells. Moreover, PNP-80 significantly promoted L929 fibroblast proliferation and migration, compared with the control group. Overall, we suggested that PNP-80 could be a promising candidate for further evaluation of its potential application on wound healing.

Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro

“…Statistical data processing was implemented through dispersion analysis with SPSS 20 software (IBM Analytics, Chicago, IL, USA). One-way ANOVA and Duncan's multiple range tests were conducted with p < 0.05 considered the level of statistical significance [18].…”

“…Cell viability was determined using an MTT assay, following a previously described procedure with slight modification [13,18]. Hs68 cells were seeded in 96-well plates (5 × 10 3 cells/well) and allowed to adhere for 24 h. The cells were incubated with 200 µL of DEME containing PNPs at concentrations of 0.5, 1.0, and 1.5 mg/mL.…”

“…The ROS generation assay was performed using a previously described method, with modification [18]. Specifically, the concentration was evaluated using a DCF-DA probe (Sigma-Aldrich, St. Louis, MO, USA).…”

“…Many studies have confirmed that the polysaccharides of P. nameko have antioxidative, antiinflammatory, hypolipidemic, and antiaging effects [15][16][17]. In in vitro experiments, a previous study showed that graded alcohol precipitation of P. nameko polysaccharides (40% v/v, PNP-40; 60% v/v, PNP-60; 80% v/v, PNP-80) revealed antioxidant abilities, such as scavenging ABTS and DPPH free radicals, chelating metal ferrous ions, and protecting L929 cells against oxidative damage induced by H 2 O 2 , as well as promoting cell migration and proliferation [18].…”

Bactericidal Potency of Bioderived Carbon and Calcium Decorated on Synthesized Copper Oxide Nanocomposite from Calotropis gigantea against Primitive Wound Associated Pathogens

“…The performance of 73.33 % for MB color removal was reached at 1-month duration (Table 5), which indicated that natural carbon and calcium wrapped CuO-500C nanoparticles could prolong the release time of free radicals in steady manner for long-term antimicrobial application and create stable environment that might provide biocompatible advantage [67]. It's also believed that overproduced hydroxyl radicals in short duration of time has reactive and hazardous free radicals which causes disturbance to wound-healing related cells and broblast [68]. Successful control on steady and slow release of free radicals such as •O 2− and •OH − from this green synthesised nanoparticles might present with biocompatible properties towards human cells and can accelerate wound healing properties [69].…”