Pholipomycin, a new member of phosphoglycolipid antibiotics. II. Physico-chemical properties and comparison with other members of this family of antibiotics.

EngineeredStreptomyces avermitilisHost for Heterologous Expression of Biosynthetic Gene Cluster for Secondary Metabolites

“…A ∼28-kb cloned segment was ligated with the integrating vector, and the desired plasmid (pPHM1) was introduced into S. avermitilis SUKA22. The resultant transformants produced ∼20 mg/L pholipomycin 30 (4) (Figure 5A), which was mainly accumulated in mycelium, and a very small amount of pholipomycin was detected from the culture broth. Interestingly, the original microorganism, S. clavuligerus ATCC 27064, did not produce pholipomycin in any culture conditions.…”

Moenomycin family antibiotics: chemical synthesis, biosynthesis, and biological activity

“…Little is known about other members of the moenomycin family. Pholipomycin 12 (produced by S. lividoclavatus ATCC29865) 38,47,48 and AC326-a 13 (from unidentified Actinomyces sp. AC326) 49 are the only compounds that were structurally characterized.…”

Nosokomycins, new antibiotics discovered in an in vivo-mimic infection model using silkworm larvae. II: Structure elucidation

“…More than 25 members of the moenomycin family have been reported from microbial metabolites. 8 However, the structures of only a few members have been fully elucidated. 5 From the structure-activity relationship of moenomycin derivatives reported previously, it has been found that moenomycin trisaccharides containing units C, E, F, G, H and I are the smallest cores to show antibiotic activity in vivo, 6 whereas moenomycin disaccharides containing units E-I still function as transglycosylase inhibitors in vitro.…”

“…8 Very recently, 1 was reported to be an intermediate of moenomycin biosynthesis, the structure of which was deduced by exact mass and MS 2 fragmentation data. 7 However, we first isolated 1 as the metabolite from the culture broth of wild-type actinomycete and obtained its full spectral data.…”