Phorbol diesters stimulate the development of an early murine progenitor cell. The burst-forming unit-megakaryocyte.

Long, Michael W.; Gragowski, L; Heffner, C H; Boxer, Laurence A.

doi:10.1172/jci111990

Cited by 83 publications

(20 citation statements)

References 23 publications

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“…The readouts of these non-megakaryocyte colonies can be totally eliminated by using more restricted sorting gates, indicating that they were derived from the contaminants from the other progenitor pools rather than from MKPs. Interestingly, Ϸ1-2% of MKP-derived colonies were large colonies that resembled the burst-forming unit-megakaryocyte (BFU-MK) described by Long et al (24). The morphology of MKP-derived CFU-MK and BFU-MK is shown in Fig.…”

Section: Mkps Exclusively Form Megakaryocyte Colonies In Vitromentioning

confidence: 68%

Characterization of mouse clonogenic megakaryocyte progenitors

Nakorn

Miyamoto

Weissman

2002

Proc. Natl. Acad. Sci. U.S.A.

142

129

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Although it has been shown that unfractionated bone marrow, hematopoietic stem cells, common myeloid progenitors, and bipotent megakaryocyte͞erythrocyte progenitors can give rise to megakaryocyte colonies in culture, monopotent megakaryocyte-committed progenitors (MKP) have never been prospectively isolated from the bone marrow of adult mice. Here, we use a monoclonal antibody to the megakaryocyte-associated surface protein, CD9, to purify MKPs from the c-kit ؉ Sca-1 ؊ IL7R␣ ؊ Thy1.1 ؊ Lin ؊ fraction of adult C57BL͞Ka-Thy1.1 bone marrow. The CD9 ؉ fraction contained a subset of CD41 ؉ Fc␥R lo CD34 ؉ CD38 ؉ cells that represent Ϸ0.01% of the total nucleated bone marrow cells. They give rise mainly to colonyforming unit-megakaryocytes and occasionally burst-forming unitmegakaryocytes, with a plating efficiency >60% at the single-cell level. In vivo, MKPs do not have spleen colony-forming activity nor do they contribute to long-term multilineage hematopoiesis; they give rise only to platelets for Ϸ3 weeks. Common myeloid progenitors and megakaryocyte͞erythrocyte progenitors can differentiate into MKPs after 72 h in stromal cultures, indicating that MKPs are downstream of these two progenitors. These isolatable MKPs will be very useful for further studies of megakaryopoiesis as well as the elucidation of their gene expression patterns.

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Section: Mkps Exclusively Form Megakaryocyte Colonies In Vitromentioning

confidence: 68%

Characterization of mouse clonogenic megakaryocyte progenitors

Nakorn

Miyamoto

Weissman

2002

Proc. Natl. Acad. Sci. U.S.A.

142

129

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“…11,[17][18][19] We detected PMA-induced expression of Pyk2 kinase, a protein expressed in megakaryocytes, 45 that was not dependent on the activity of the MEK/MAPK pathway. Since PMA-stimulated differentiation of K562 cells mimics the pathway of natural megakaryocyte precursors, 6 it remains to be detemined whether Pyk2 expression is regulated in a similar fashion in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…These latter events have been observed in response to a number of cell activators. 6 Megakaryocytic and erythroid differentiation has been shown to be a property of several established cell lines including K562, HEL and MEG-01 cells. [7][8][9][10] Differentiation of these cell lines can be modulated by treatment with PMA, hemin or butyric acid.…”

Section: Introductionmentioning

confidence: 99%

PMA-induced phenotypic changes in K562 cells: MAPK-dependent and -independent events

Shelly¹,

Petruzzelli

Herrera³

1998

Leukemia

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“…Because of the very low incidence we were not able to further characterize these colonies, and their single cell origin has not yet been established. However, in analogy to the developmentally earliest megakaryocytic progenitors in the murine system (BFU-MK), which are felt to antedate the more commonly observed CFU-MK and which form large colonies with a distinct subcolony development [67] Figure 4 summarizes these observations and offers a hypothetical differentiation sequence for committed megakaryocytic progenitors along a developmental continuum that is reminiscent of differentiation in the erythroid lineage [15]: cells of the different subtypes of colonies might represent progeny of subpopulations of clonable megakaryocytic progenitor cells along their way from uncommitted pluripotent stem cells to the nonproliferating compartment of megakaryocytes. The more primitive the progenitor cell, the higher the proliferative potential and the larger the colonies formed.…”

Section: Clonogenic Assays For Committed Human Megakaryocytic Progenimentioning

confidence: 99%

Human megakaryocytic progenitor cells

1987

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Megakaryocytopoiesis represents one of several differentiation pathways that hematopoietic stem cells may enter. Cells representing intermediate stages of differentiation between pluripotent stem cells and maturing megakaryocytes are called megakaryocytic progenitor cells. They are identified in human bone marrow and peripheral blood by their ability to proliferate in culture (colony forming unit-megakaryocyte, CFU-M); at some point they lose the capacity for cell division and acquire the ability for endoreduplication of DNA, a phenomenon that is unique to the megakaryocyte lineage. This review summarizes current understanding of the biology of human megakaryocytic progenitor cells, including characterization of their proliferation potentials, their antigenic determinants, and the mechanisms that govern their proliferation and maturation. Finally the involvement of CFU-M in various disorders of thrombopoiesis is discussed.

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Phorbol diesters stimulate the development of an early murine progenitor cell. The burst-forming unit-megakaryocyte.

Cited by 83 publications

References 23 publications

Characterization of mouse clonogenic megakaryocyte progenitors

Characterization of mouse clonogenic megakaryocyte progenitors

PMA-induced phenotypic changes in K562 cells: MAPK-dependent and -independent events

Human megakaryocytic progenitor cells

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