Phorbol ester stimulates macrophage invasion of fibrin matrices

Castellucci, Mario; Montesano, Roberto

doi:10.1002/ar.1092200102

Cited by 10 publications

(3 citation statements)

References 56 publications

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“…This progressively induced an inflammatory infiltrate, of which the MC/Mph population represented a main component [99]. We observed a system of tissular "tunnels" with a cross-sectional size comparable to the diameter of the infiltrating cells [97], similar to those produced by the penetration of phorbol ester-activated Mph in a fibrin matrix in vitro [100] (phorbol ester also induces the expression of MME [101]). …”

Section: Intercellular Cooperation During Formation Of Capillariesmentioning

confidence: 58%

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Role of monocytes and macrophages in angiogenesis

Moldovan

2005

Mechanisms of Angiogenesis

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Section: Intercellular Cooperation During Formation Of Capillariesmentioning

confidence: 58%

“…The nature and fate of these tunnels was not considered so far, despite the previous empirical evidence for their existence [100]. They are tubular spaces of lower density, containing extracellular fluid and degradation products of the ECM.…”

Section: Intercellular Cooperation During Formation Of Capillariesmentioning

confidence: 99%

Role of monocytes and macrophages in angiogenesis

Moldovan

2005

Mechanisms of Angiogenesis

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“…The initial morphological studies on Matrigel's ability to regulate Sertoli cell differentiation, testicular cord formation, and germ cell development (Hadley et al, 1985) have since expanded to include biological phenomenon such as cell attachment, migration, and invasion. Tumor cell locomotion is important in cell invasion (Grimstad, 1987;Maslow, 1987) and has been linked to various normal extracellular matrix molecules (Chung et al, 1988) as well as to abnormal molecules such as phorbol esters (Castellucci and Montesano, 1988). Laminin and the cell receptor for laminin are becoming increasingly important in the emerging tumor cell migration hypothesis (Wewer et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Artificial matrix barriers: A diffusion study utilizing dextrans and microspheres

Persky

Hendrix

1990

Anat. Rec.

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Eight artificial matrices (AMs) were evaluated for the ability to restrict the passage of diffusion probes. Three AMs were composed exclusively of interstitial type I collagen (Col I) and differed from each other in thickness only. Four AMs consisted of reconstituted basement membrane (RBM) -coated polycarbonate filters (containing 10 microns diameter pores) and also only differed in thickness. One AM consisted of an uncoated 10 microns pore polycarbonate filter. The diffusion probes were uncharged fluorescein isothiocyanate-labeled dextrans, having molecular weights of 17,900, 42,000, 71,200, and 148,900 and negatively charged latex microspheres, having diameters of 0.08, 0.30, and 0.95 microns. Probes were applied to the AMs, incubated for 72 hr at 37 degrees C, and then analyzed spectrophotometrically. Dextran passage was increasingly restricted for Col I matrices as either molecular weight or collagen thickness increased (range 7% to 0.7%). Thin RBM-coated filters were more permeable to dextrans (range 100% to 30%) than Col I matrices. The diffusion rate of microspheres for Col I matrices (range 3.5% to 0) was similar to both thick and thin RBM-coated filters (range 4% to 0). The uncoated filter permitted the most diffusion for both dextrans and microspheres (range 100% to 7%). These data demonstrate that the AMs presented in this study will allow direct observation of the degradative and migratory potential of cells in vitro as they interact with various extracellular matrices.

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Extracellular matrix influences hormone and protein production by human chorionic villi

Castellucci¹,

Kaufmann²,

Bischof

1990

Cell Tissue Res

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Increasing evidence confirms that the extracellular matrix greatly influences cell behaviour and function. Collagen and fibrin are in contact with trophoblast throughout pregnancy. To investigate whether these two matrices influence hormone production by the trophoblast, explants from first-trimester chorionic villi were cultured for up to 30 days either a) in medium with agitation, b) embedded in type-I collagen (three-dimensional gels), or c) embedded in fibrin (three-dimensional gels). The supernatant culture medium was changed every 48 h and tested by radioimmunoassay for hCG, progesterone and pregnancy-associated plasma protein A. In addition, after 3, 7, 15, and 30 days of culture villi were fixed and studied by light and electron microscopy. Embedding in the extracellular matrix showed higher and longer-lasting production rates of all measured products and superior structural preservation as compared to cultures with agitation. Collagen matrix proved to be superior to fibrin. As established by several tests, this difference was neither due to thrombin used to polymerize fibrinogen, nor to differences in the diffusion rates through the two different matrices used. We conclude that extracellular matrix, particularly collagen, influences the synthesis of trophoblastic products. Embedding of the villous explants in three-dimensional gels constitutes a new method for long-term cultures of chorionic villi.

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Phorbol ester stimulates macrophage invasion of fibrin matrices

Cited by 10 publications

References 56 publications

Role of monocytes and macrophages in angiogenesis

Role of monocytes and macrophages in angiogenesis

Artificial matrix barriers: A diffusion study utilizing dextrans and microspheres

Extracellular matrix influences hormone and protein production by human chorionic villi

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