Phosphatase Activity of Anaerobic Organisms

Porschen, Richard K.; Spaulding, Earle H.

doi:10.1128/aem.27.4.744-747.1974

Cited by 18 publications

(11 citation statements)

References 3 publications

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“…Acid phosphatase activity appears to be a consistent property of CI. perfringens itself and, furthermore, Porschen & Spaulding (1974) obtained positive phosphatase reactions over a wide range of pH values. Whether this indicates one or several enzymes in CI.…”

mentioning

confidence: 83%

A Study of Rapid and Simplified Confirmatory Tests for Clostridium perfringens

Mead¹,

León

Adams³

1981

Journal of Applied Bacteriology

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Strains of Clostridium perfringens and culturally similar species which also may grow on selective isolation media for this organism were examined by conventional confirmatory tests, the API ZYM system and by individual tests for phosphatase and glutamic acid decarboxylase activity. API ZYM tests, involving 19 different enzymes, confirmed the known similarity between Cl. perfringens, Cl. absonum, Cl. paraperfringens and Cl. sardiniensis but effectively distinguished this group from Cl. bifermentans, Cl. celatum, Cl. perenne and Cl. sordellii. A similar separation was achieved by a single test for acid phosphatase which could be applied to individual colonies on a plating medium. Because the acid phosphatase test was found to be of greater value than nitrate reduction in distinguishing Cl. perfringens, it could replace the latter in the usual series of confirmatory tests. It is suggested that strains from Cl. perfringens isolation media should be screened for acid phosphatase activity at the purification stage and only positive strains subjected to further tests. It was found that Cl. perfringens could not be distinguished from the other species on the basis of glutamate decarboxylase activity.

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mentioning

confidence: 83%

A Study of Rapid and Simplified Confirmatory Tests for Clostridium perfringens

Mead¹,

León

Adams³

1981

Journal of Applied Bacteriology

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“…Acid phosphatase test. Ueno et al 25and Porschen and Spaulding (20) reported that the ability of C. perfringens to produce acid phosphatase differentiated it from a number of other species of Clostridium. Blazevic and Ederer (2) described a test for phosphatase activity using phenolphthalein diphosphate as a substrate.…”

Section: Methodsmentioning

confidence: 99%

“…With the freshwater suspension, the recoveries after 24 h were approximately equal under both conditions; however, they were also about 23% lower than the respective recoveries at zero hour. The most likely explanation for these results is that (i) 20 to 30% of the C. perfringens population in the sewage sample were vegetative cells, (ii) because of factors in both sewage and seawater, they survive for periods up to 24 h in a sewage-sea- b Assay performed inside anaerobic tent.…”

Section: Fringensmentioning

confidence: 96%

Membrane filter enumeration method for Clostridium perfringens

Bisson¹,

Cabelli²

1979

Appl Environ Microbiol

147

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A membrane filter procedure has been developed for the rapid quantitation of C. perfringens in the aquatic environment. Background growth is inhibited by the use of D-cycloserine, polymyxin B sulfate, and incubation at 450C. Differential characteristics include the fermentation of sucrose, production of acid phosphatase, and the absence of ,B-D-glucosidase activity. The medium is prepared as follows (in grams per 100 ml of distilled water): tryptose, 3.0; yeast extract, 2.0; sucrose, 0.5; L-cysteine, 0.1; MgSO4.7H20, 0.01; bromocresol purple, 0.004; and agar, 1.5. The ingredients are dissolved, and the pH is adjusted to 7.6. After autoclaving at 1210C for 15 min, the medium is allowed to cool at 500C, and the following are added per 100 ml: D-cycloserine, 40 mg; polymyxin B sulfate, 2.5 mg; indoxyl-fl-D-glucoside, 60 mg, 2.0 ml of a filter-sterilized 0.5% phenolpthalein diphosphate solution; and 0.2 ml of a filter-sterilized 4.5% FeCl3-6H20 solution. Enumeration of C. perfringens in a water sample is completed within 18 to 24 h. The verification of typical colonies was 93%. The average recovery from peptonewater spore suspensions of five strains was 79%, and that from filter-sterilized seawater suspensions was 90%. The precision of the method was approximately equal to that expected from random error alone. Confirmed recoveries of C. perfringens from water and sewage samples generally were greater than those by the Bonde pour tube method.

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“…Hydrolytic enzymes are produced by a number of species of anaerobic bacteria. These enzymes include hyaluronidase (12)(13)(14), chondroitin sulfatase (12)(13)(14), neuraminidase (8), heparinase (3,13), protease (12), elastase (12), collagenase (4,14), fibrinolysin (12), deoxyribonuclease (10), phosphatase (11,12), penicillinase (9,12), lecithinase (12), and lipase (12). There is some evidence that hydrolytic enzymes secreted by certain species of anaerobic bacteria play a role in the infectious process.…”

mentioning

confidence: 99%

Hydrolytic enzymes of anaerobic bacteria isolated from human infections

Steffen¹,

Hentges²

1981

J Clin Microbiol

104

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Thirty-three strains of anaerobic bacteria isolated from human clinical specimens were examined for the presence of heparinase, hyaluronidase, chondroitin sulfatase, gelatinase, collagenase, fibrinolysin, lecithinase, and lipase activities. Pronounced heparinase activity was limited to species of the genus Bacteroides. A number of species of the genera Bacteroides and Clostridium produced hyaluronidase and chondroitin sulfatase. Gelatinase, collagenase, and fibrinolysin activities were encountered in isolates of the genera Bacteroides, Clostridium, and Peptostreptococcus. All strains capable of degrading collagen also hydrolyzed other protein substrates. Lipolytic activity was minimal among these anaerobic bacteria. No specific hydrolytic activity was consistently associated with the isolates.

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Phosphatase Activity of Anaerobic Organisms

Cited by 18 publications

References 3 publications

A Study of Rapid and Simplified Confirmatory Tests for Clostridium perfringens

A Study of Rapid and Simplified Confirmatory Tests for Clostridium perfringens

Membrane filter enumeration method for Clostridium perfringens

Hydrolytic enzymes of anaerobic bacteria isolated from human infections

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