Phosphatase Histochemistry of the Eye

ULTRACYTOCHEMICAL LOCALIZATION OF OUABAIN-SENSITIVE, POTASSIUM-DEPENDENT <i>P</i>-NITROPHENYLPHOSPHATASE ACTIVITY IN THE GUINEA PIG RETINA

“…Previous cytochemical studies of Mg-ATPase activity in the retina, one at the light microscopic level (36), another at the level of electron microscopy (38), were also carried out with the Wachstein-Meisel procedure. No inhibitory effect of ouabain was found (36), and the reaction products were independent of Na and K ions (38). Stimulated by these failures, the validity of the Wachstein-Meisel type procedure itself in Na-K-ATPase cytochemistry has been challenged (22, 48-50, 58-60, 66).…”

Expression of Plasma Membrane Alkaline Phosphatase in Normal and Regenerating Choriocapillaris in the Rabbit

“…These changes are not due to a direct effect of sodium iodate on the endothelium, which we have addressed previously |7|. Earlier studies using liglu-microscopic histochcmical techniques to localize AP in ocular tis sue [11,12] reported intense activity in the CC of several mammals, including rabbits. The results of our study are not consistent with these findings.…”

Ocular Changes of Glycogen Storage Disease Type I

“…Patients suffer repeated episodes of hypoglycemia, elevated blood lipid levels and xanthomas on the extensor surface of the extremities [3,4]. Lessell and Kuwabara [5] and Durlu et al [6] have reported finding the enzyme in the inner parts of Müller cells, retinal pigment epithelium and corneal epithelium in the rabbit eye. We ex amined two patients with GSD I, which were clinically clas sified into types la (case 2) and b (case 1), and noted the oc ular changes associated with the distribution of enzyme among the ocular tissues.…”