Phosphatase Hydrolysis of Organic Phosphorus Compounds

Abstract

Phosphatases are diverse groups of enzymes that deserve special attention because of their significant roles in organic phosphorus (OP) mineralization to inorganic available forms (Pi). This work 1) compared the catalytic potentials of commercially acid phosphatase from wheat germ, sweet potato, and potato, and alkaline phosphatase from E. coli; 2) demonstrated that the rate of hydrolysis, catalytic efficiency, thermal stability, and optimal pH of these enzymes depended on enzyme sources and the stereochemical or stereoisomeric structures of the substrates; 3) revealed that both acid and alkaline phosphatases exhibited broad range of substrate hydrolysis with high affinity for p-nitrophenyl phosphate bis (cyclohexylammonium) than the widely used p-nitrophenyl phosphate disodium hexahydrate for phosphatase assay. Sweet potato had relatively higher reaction kinetics (Vmax, K m , K cat , K cat /K m ) values with most substrates tested. The order of catalytic activity was in the order: sweet potato > wheat germ > potato, while the order of substrate hydrolyzed was: PNPBC > PNP > PNP2A2E > DG6P2Na > DG6PNa > Bis-PNP > phytate. The optimum pH for the acid phosphatase was observed to be 5.0. Generally, the activity of alkaline phosphatase was similar to that of acid phosphatase with optimal pH between 10 and 13, depending on the substrates. Knowledge derived from this work would be helpful in enzyme catalysis in soils and water environments.

Effect of Vesicular Arbuscular Mycorrhizal (VAM) Fungus and Rock-Phosphate Application on the Growth and Biomass of Moringa oleifera Lam. Seedlings under Salinity Stress

“…This may owing to the ability of mycorrhiza to increase root surface area to uptake mineral contents and make phosphorus absorpable by plant roots. This result was in agreement with the finding of Pagano et al (2010) who reported that VAM colonization was significantly higher with the inoculated seedlings versus non-inoculated ones (control) and Tazisong et al (2015) who said that Phosphatases are responsible for the hydrolysis of a range of organic P compounds and provide mineral phosphate to the plant. Furthermore, Matias et al (2009) reported that the intensity of VAM colonization was also stimulated by plant growth.…”

The Interaction between Arbuscular Mycorrhizal Fungi and Endophytic Bacteria Enhances Plant Growth of Acacia gerrardii under Salt Stress

“…Phosphatases are responsible for the hydrolysis of a range of organic P compounds and provide mineral phosphate to the plant (Tazisong et al, 2015 ). In our study, increased acid and ALP was observed in A. gerrardii grown under salt stress.…”

Phylogenetic-scale disparities in the soil microbial diversity–ecosystem functioning relationship

“…b Bacterial phylogenetic tree depicting the evolutionary relationships between 16 phyla present in the study system. At 2500 Myr, coloured circles indicate eight extant phyla and the most recent common ancestors of the clades Chloroflexi-OD1-TM7 and Planctomycetes-Verrucomicrobia-Gemmatimonadetes -Acidobacteria-Nitrospira same family (e.g., phosphatases) depending on their biological origin [75]. The more effective niche filling with differentiated recent lineages would ultimately explain the correlation between high PD and EFs [25].…”