Phosphatases: The Critical Regulator of Abiotic Stress Tolerance in Plants

Sahoo, Sripati Abhiram; Raghuvanshi, Rishiraj; Srivastava, Ashish Kumar; Suprasanna, Penna

doi:10.1007/978-3-030-48733-1_10

Cited by 3 publications

(2 citation statements)

References 111 publications

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“…PP2CAs is a positive ABA signalling regulator (52) but to our knowledge, no reports exist on the functional relevance of its own phosphorylation. AT3G51470 is also a PP2C G family member, was only rhythmic in dataset I and no functional information is available (53). The final PP2C POLTERGEIST (POL, Figure S 7C) is involved in stem cell regulation (54).…”

Section: Rhythmically Phosphorylated Kinases and Phosphatasesmentioning

confidence: 99%

The circadian clock gene circuit controls protein and phosphoprotein rhythms inArabidopsis thaliana

Krahmer

Hindle

Perby

et al. 2019

Preprint

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The 24-hour rhythmicity in the levels of many eukaryotic mRNAs contrasts with the long half-lives of most detected proteins. Such stable molecular species are not expected to reflect the RNA rhythms, emphasizing the need to test the circadian regulation of protein abundance and modification. Here we present circadian proteomic and comparable phosphoproteomic time-series from Arabidopsis thaliana plants, estimating that 0.4% of quantified proteins and a much larger proportion of quantified phosphosites were rhythmic under constant light conditions. Approximately half of the quantified phospho-sites were most phosphorylated at subjective dawn, when SnRK1 protein kinase was a candidate regulator.A CCA1-over-expressing line that disables the plant clock gene circuit lacked most or all circadian protein phosphorylation, indicating that the phospho-dawn is regulated by the canonical clock mechanism. The few phospho-sites that fluctuated despite CCA1-over-expression still tended to peak in abundance close to subjective dawn, indicating that their temporal regulation was less dependent on the clock gene circuit. To test the potential functional relevance of our datasets, we conduct phosphomimetic experiments using the bifunctional enzyme fructose-6-phosphate-2-kinase / phosphatase (F2KP), as an example. The clock gene circuit controls diverse protein targets in metabolism and physiology via phosphorylation, including where the bulk abundance of the cognate proteins is arrhythmic.

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Section: Rhythmically Phosphorylated Kinases and Phosphatasesmentioning

confidence: 99%

The circadian clock gene circuit controls protein and phosphoprotein rhythms inArabidopsis thaliana

Krahmer

Hindle

Perby

et al. 2019

Preprint

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“…PP2C G1 (Figure S 9A) is involved in ABA dependent salt stress response and, in contrast toPP2CAs is a positive ABA signalling regulator (54) but to our knowledge, no reports exist on the functional relevance of its own phosphorylation. AT3G51470 is also a PP2C G family member, was only rhythmic in dataset I and no functional information is available(55). The final PP2C POLTERGEIST (POL, FigureS 9C) is involved in stem cell regulation(56).…”

mentioning

confidence: 99%

The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana

Krahmer

Hindle

Perby

et al. 2022

Molecular & Cellular Proteomics

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Twenty-four-hour, circadian rhythms control many eukaryotic mRNA levels, whereas the levels of their more stable proteins are not expected to reflect the RNA rhythms, emphasizing the need to test the circadian regulation of protein abundance and modification. Here we present circadian proteomic and phosphoproteomic time series from Arabidopsis thaliana plants under constant light conditions, estimating that just 0.4% of quantified proteins but a much larger proportion of quantified phospho-sites were rhythmic. Approximately half of the rhythmic phospho-sites were most phosphorylated at subjective dawn, a pattern we term the “phospho-dawn.” Members of the SnRK/CDPK family of protein kinases are candidate regulators. A CCA1 -overexpressing line that disables the clock gene circuit lacked most circadian protein phosphorylation. However, the few phospho-sites that fluctuated despite CCA1 -overexpression still tended to peak in abundance close to subjective dawn, suggesting that the canonical clock mechanism is necessary for most but perhaps not all protein phosphorylation rhythms. To test the potential functional relevance of our datasets, we conducted phosphomimetic experiments using the bifunctional enzyme fructose-6-phosphate-2-kinase/phosphatase (F2KP), as an example. The rhythmic phosphorylation of diverse protein targets is controlled by the clock gene circuit, implicating posttranslational mechanisms in the transmission of circadian timing information in plants.

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Drought Stress: Responses and Mechanism in Plants

Pamungkas¹,

Suwarto

Suprayogi

et al. 2022

RAS

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The function of water for plants is crucial, including playing the roles in metabolic reactions. The aims of this article are to give information on the effects of drought stress on plant morphology, physiology, and biochemistry, as well as mitigation methods in drought stress management for plant production. Plants manage drought stress using a mechanism, namely drought escape, drought avoidance and drought tolerance. Drought escape is the ability of plants to accelerate flowering or life cycle, drought avoidance is the ability of plants to reduce water loss and increase water absorption through morphological changes in the root system, drought tolerance is the plant adaptation to drought by changes in plant physiological and biochemical processes. Physiological changes that occur include closing the stomata and decreased photosynthesis. The biochemical responses include the synthesis of solute compounds as a form of osmotic adjustment in the cell called osmotic adjustment to reduce water loss from the cell. The biochemical indicators are the increased concentrations of abscisic acid (ABA), proline, and sugar (trehalose). ABA acts as a signal to stimulate stomatal closure to reduce the transpiration rate. Proline is an indicator of plants adapting to drought stress, playing a role in the osmotic adjustment of cells to retain in the cell. Trehalose is a compatible sugar acting as an osmoprotectant, it can maintain the integrity of cell membranes (water replacement) and form hydrogen bonds (water entrapment). Plants under drought stress conditions can adapt by making morphological, physiological, and biochemical responses by osmotic adjustment. These conditions need to be managed so that appropriate strategies and technologies are needed as mitigation measures.

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Phosphatases: The Critical Regulator of Abiotic Stress Tolerance in Plants

Cited by 3 publications

References 111 publications

The circadian clock gene circuit controls protein and phosphoprotein rhythms inArabidopsis thaliana

The circadian clock gene circuit controls protein and phosphoprotein rhythms inArabidopsis thaliana

The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana

Drought Stress: Responses and Mechanism in Plants

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