Phosphate Dependence of Monosaccharide Transport in
            <i>Nocardia</i>

Cerbón, Jorge; Ortigoza-Ferado, J

doi:10.1128/jb.95.2.350-354.1968

Cited by 8 publications

(2 citation statements)

References 12 publications

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“…In addition, they concluded that glycerol uptake was by facilitated diffusion or active transport at concentrations of glycerol below 80 ,uM, and by "free diffusion" at higher glycerol concentrations. Cerbon and Ortigoza-Perado (3) observed that in N. asteroides and N. brasiliensis the uptake of D-glucose and D-mannose was dependent upon an optimal supply of exogenous phosphate. They also demonstrated that D-glucose was taken up by facilitated diffusion.…”

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confidence: 98%

Regulation of Substrate Uptake in Nocardia erythropolis

Pang

Bradley

1974

J Bacteriol

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The regulation of uptake of glucose (GLU), glycerol (GLY), mannitol (MTL), and succinate (SUC) has been examined in Nocardia erythropolis 305. The apparent Km values of the uptake activities of cells subcultured in a medium with the corresponding substrate as the sole carbon source were 205, 48, 8.7, and 36 gM for GLU, GLY, MTL, and SUC, respectively. GLU and GLY uptake activities were constitutive, although there was evidence for an additional inducible component in GLY uptake. Moreover, MTL and SUC uptake activities were inducible. MTL uptake activity was markedly induced by cultivation in MTL medium and partially induced by growth in GLU medium, whereas SUC uptake was induced only by cultivation in SUC medium. SUC added to MTL medium partially repressed the formation of, or inhibited the activity of, MTL uptake. When not induced, uptake of MTL and SUC was proportional to the substrate concentration. The induced uptake of MTL and SUC and the constitutive uptake of GLU were energy dependent and carrier mediated. Uptake of GLY, constitutively or when induced, was also carrier mediated.

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confidence: 98%

Regulation of Substrate Uptake in Nocardia erythropolis

Pang

Bradley

1974

J Bacteriol

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“…There is evidence for mutual cross-resistance between arsenite and arsenate in Pseudomonas pseudomallei (1). Neither proteins, ribonucleic acid, nor deoxyribonucleic acid could be shown to be the receptors for arsenite in P. pseudomallei (1), and arsenate can replace phosphate in the monosaccharide transport system of Nocardia which is completely dependent on this anion (4). It is also known that the lipid extract of this microorganism contains most of the bound arsenic (5).…”

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Arsenic-Lipid Complex Formation During the Active Transport of Arsenate in Yeast

Cerbón

1969

J Bacteriol

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In studying formation of an arsenic-lipid complex during the active transport of 74As-arsenate in yeast, it was found that adaptation of yeast to arsenate resulted in cell populations which showed a deficient inflow of arsenate as compared to the nonadapted yeast. Experiments with both types of cells showed a direct correlation between the arsenate taken up and the amount of As-lipid complex formed. 74Asarsenate was bound exclusively to the phosphoinositide fraction of the cellular lipids. When arsenate transport was inhibited by dinitrophenol and sodium azide, the formation of the As-lipid complex was also inhibited. Phosphate did not interfere with the arsenate transport at a non-inhibitory concentration of external arsenate (10r M). The As-adapted cells but not the unadapted cells were able to take up phosphate when growing in the presence of 12 M arsenate.It has been well established that arsenate enters the yeast cell via a constitutive transport system which is normally responsible for phosphate uptake; but, in contrast to phosphate, arsenate continuously inactivates the transport system in an irreversible manner, so that the uptake eventually ceases (7). This evidence, together with the possibility of changing the cells from having a highly efficient arsenate transport system [arsenate-sensitive (AsS) cells] to deficient ones [arsenate-resistant (AsR) cells] (1), would allow an objective analysis of the permeation process and perhaps the identification of permeation components by correlating compositional with permeability changes.There is evidence for mutual cross-resistance between arsenite and arsenate in Pseudomonas pseudomallei (1). Neither proteins, ribonucleic acid, nor deoxyribonucleic acid could be shown to be the receptors for arsenite in P. pseudomallei (1), and arsenate can replace phosphate in the monosaccharide transport system of Nocardia which is completely dependent on this anion (4). It is also known that the lipid extract of this microorganism contains most of the bound arsenic (5). The present study was undertaken to determine whether the same type of As-lipid complex is formed in yeasts during the arsenate transport and, if so, whether its formation is altered in the same way as the arsenate system, by development of arsenate resistance and by the effect of metabolic inhibitors. MATERIALS AND METHODSMicroorganisms and conditions of culture. AsS cells of Saccharomyces carlsbergensis ATCC 9080 and their corresponding AsR yeast cultures were utilized.The sensitive cells were grown on nutrient broth containing 1% yeast extract plus 1% maltose at 28 C.The AsR cells were obtained by growing the sensitive cells in the same medium to which 102 M arsenate was added. Cultivation was at 28 C under standing conditions. When marked proliferation of microorganisms was observed after 6 days, the cells were separated by centrifugation and inoculated into fresh medium containing the same concentration of arsenate. After three to four passages, the prolonged lag typical of adapting cultures was reduced,...

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The influence of pH and temperature on the limited rotational freedom of the structured water and lipid hydrocarbon chains of natural membranes

Cerbón

1970

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Phosphate Dependence of Monosaccharide Transport in Nocardia

Cited by 8 publications

References 12 publications

Regulation of Substrate Uptake in Nocardia erythropolis

Regulation of Substrate Uptake in Nocardia erythropolis

Arsenic-Lipid Complex Formation During the Active Transport of Arsenate in Yeast

The influence of pH and temperature on the limited rotational freedom of the structured water and lipid hydrocarbon chains of natural membranes

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