Phosphate (Pi) uptake and remobilisation within plant in Brassica spp. under different Pi availabilities input

Abstract

Phosphorus (P) is one of six essential macronutrients in plants to ensure crop growth and productivity. Plants only acquire phosphorus in the form of inorganic phosphate (Pi) known as orthophosphate, mainly in the form of H2PO4 −. The high demand of Pi in fertilisers for agriculture use contributed to environmental pollution. Therefore, it is important to understand the Pi uptake from the soil and remobilisation within the plant to increase P use efficiency (PUE). In this study, the experiments were undertaken to look at the variation of Pi uptake by Brassica spp. under different soil Pi availability. Two soil Pi concentrations, P1 (0 g L−1) and P4 (0.225 g L−1) were selected to represent the low and high Pi treatment, respectively. B. napus plants grown in compost with both high and low Pi treatments were harvested at four different parts of the plant (leaf, stem, flower, and pod silique and seed) at six different growth stages, namely two-leaf stage, four-leaf stage, flowering stage, first flower opens stage, seed filling stage and maturity stage. The results of free Pi concentration in P4 (0.225 g L−1) were significantly higher than P1 (0 g L−1) (P ≤ 0.01). Remobilisation of Pi determined by total P contents of plants treated with P1 and P4 both showed similar trends, increased P contents up to 50% of plant P at harvest 5 (seed stage), and loss of P at leaf, stem and flower tissues during senescence at maturity stage. Under deficiency, leaf Pi concentration which determined the internal Pi redistribution showed Pi was redistributed from source (mature organs) to sink (developing tissues) Pi tissues. These results provide insights on plant nutrient status and can be used for further investigation for crop optimal production under low Pi availability.

Selection of reference genes for qPCR analysis and variations in the expression of Phosphate (Pi) responsive genes in Brassica rapa

“…Alteration of metabolic pathways and reallocation of internal Pi facilitate Pi remobilization to different organs and cellular compartments. These responses are in line with the ndings that the plant experienced physiological changes through the biomass allocation and redistribution of P to the developing organ in matured plants 53 . The high expression pro les of SUC2, MATE, ALMT1, MMDH1, and PAP12 indicate responses in Pi signaling, transport and extracellular Pi scavenging to increase Pi availability occurred in B. rapa under Pi-starvation.…”