Phosphate regulated proteins of Xanthomonas citri subsp. citri: A proteomic approach

Structural features of PhoX, one of the phosphate-binding proteins from Pho regulon of Xanthomonas citri

“…In a previous paper, we showed that the phoX gene did not belong to the operon that included pstSCABphoU genes encoding the ATP-Binding Cassette transporter for phosphate uptake and the negative regulator of the system, the protein PhoU. In addition, we showed that PstS and PhoX were upregulated, at different levels, under phosphate starvation [6]. Since all residues involved in ligand binding were conserved in the two proteins (Fig 3A), we looked to determine if the interface of these proteins that interact with the membrane components PstC and PstA were similar.…”

“…Previously, we characterized the putative Pho regulon proteins from the plant pathogenic bacterium Xanthomonas citri , the causative agent of citrus canker disease, and showed the presence of two putative phosphate-binding proteins, PstS (XAC1577) and PhoX (XAC1578) that share 70% amino acid sequence identity [6]. Proteomic studies revealed that under phosphate limitation, PhoX and PstS were highly induced with fold change values of 43.5 and 39.1 respectively, indicating that both proteins have an active role in the Pst system.…”

Activation of PhoBR under phosphate‐rich conditions reduces the virulence of Xanthomonas oryzae pv. oryzae

“…The growth reduction of the phoR mutant implies that Xoo specifically switches to a persistence-like growth status under phosphate-limited conditions. This was also verified by a previous proteomic study on X. citri, which showed that transport and metabolism-associated proteins were over-represented amongst the differentially expressed proteins identified from X. citri cultured in phosphate-rich and phosphate-limited media (Pegos et al, 2014). We propose that growth reduction, which resulted from nutrient transport and metabolic process readjustments, can be another strategy to adapt to phosphate limitation.…”

Caracterização estrutural e análises funcionais das proteínas periplasmáticas NrtT e PotF de transportadores do tipo ABC de Xanthomonas axonopodis pv. citri.

“…Além da captação de nutrientes e transporte, os transportadores ABC estão envolvidos em outros processos como exportação de toxinas e resistência à drogas (HIGGINS, 2007;LAGE, 2003), reparo do DNA (DOOLITTLE et al, 1986;HIGGINS et al, 2001), patogênese e infecção (LEWIS et al, 2012) , 2001;HIGGINS, 1990;KERR, 2002;LINTON, 2007;MOUSSATOVA et al, 2008;REES et al, 2009;WANNER;SOPPA, 1999) (KEMNER et al, 1997;LEWIS et al, 2012;LI et al, 2011). Apesar desta relevância, poucos são os estudos evidenciando o papel destes transportadores em Xanthomonas (BALAN et al, 2008;ARAÚJO et al, 2013;PEGOS et al, 2014). A primeira descrição da contribuição de um transportador ABC na virulência foi descrita em Agrobacterium tumefaciens com o transportador de açúcares, glicose e galactose.…”

“…), maltose (MEDRANO et al, 2014;SOUZA et al, 2009), molibdato (BALAN et al, 2008SANTACRUZ-PEREZ et al, 2013), tolueno, sulfato (PEREIRA et al, in press. ), oligopeptídeos (MOUTRAN et al, 2007), fosfato (PEGOS et al, 2014) e alcano sulfonatos (ARAÚJO et al, 2013). Na…”