Phosphate-Solubilizing Bacteria Isolated from Phosphate Solid Sludge and Their Ability to Solubilize Three Inorganic Phosphate Forms: Calcium, Iron, and Aluminum Phosphates

Aliyat, Fatima Zahra; Maldani, Mohamed; Guilli, Mohammed El; Nassiri, Laila; Ibijbijen, Jamal

doi:10.3390/microorganisms10050980

Cited by 46 publications

(43 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been reported that acidification modifies the precipitation/dissolution equilibrium of P and calcium sequestration by organic acids, and this results in P solubilizing and bioavailability [ 5 , 7 ]. Aliyat et al [ 34 ] recently showed the decreasing pH of culture medium through organic acids production (formic, propionic, isobutyric, butyric, isovaleric, caproic, and heptanoic acids) by PSB strains. Benbrik et al [ 19 ] pointed out that in a liquid culture medium, microbial biomass respiration generates CO 2, -producing carbonic acid, creating acidic conditions and, consequently, bacterial growth inhibition, as well solubilization activity.…”

Section: Resultsmentioning

confidence: 99%

Effect of pH and Carbon Source on Phosphate Solubilization by Bacterial Strains in Pikovskaya Medium

et al. 2022

View full text Add to dashboard Cite

Phosphate-solubilizing bacteria (PSB) transform precipitated inorganic phosphorus into soluble orthophosphates. This study evaluated the efficiency of tricalcium and iron phosphate solubilization in Pikovskaya medium using five bacterial strains (A1, A2, A3, A5, and A6) cultured in acidic and alkaline pH levels. The bacterial strain that proved to be more efficient for P solubilization and was tolerant to pH variations was selected for assessing bacterial growth and P solubilization with glucose and sucrose in the culture medium. The bacterial strains were identified through 16S rRNA gene sequencing as Pseudomonas libanensis A1, Pseudomonas libanensis (A2), Bacillus pumilus (A3), Pseudomonas libanensis (A5), and Bacillus siamensis (A6). These five bacterial strains grew, tolerated pH changes, and solubilized inorganic phosphorus. The bacterial strain A3 solubilized FePO4 (4 mg L−1) and Ca3(PO4)2 (50 mg L−1). P solubilization was assayed with glucose and sucrose as carbon sources for A3 (Bacillus pumilus MN100586). After four culture days, Ca3(PO4)2 was solubilized, reaching 246 mg L−1 with sucrose in culture media. Using glucose as a carbon source, FePO4 was solubilized and reached 282 mg L−1 in six culture days. Our findings were: Pseudomonas libanensis, and Bacillus siamensis, as new bacteria, can be reported as P solubilizers with tolerance to acidic or alkaline pH levels. The bacterial strain B. pumilus grew using two sources of inorganic phosphorus and carbon, and it tolerated pH changes. For that reason, it is an ideal candidate for inorganic phosphorus solubilization and future production as a biofertilizer.

show abstract

Section: Resultsmentioning

confidence: 99%

Effect of pH and Carbon Source on Phosphate Solubilization by Bacterial Strains in Pikovskaya Medium

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Increasing soil P concentrations via inorganic and organic soil P mobilization pools involve changes in pH and the release of P-mobilizing compounds such as carboxylates and phosphatases, which are exuded by the roots themselves or by micro-organisms in the rhizosphere. However, the selection of an efficient PSB is crucial, as it practically increases P in the plant rhizosphere (Aliyat et al, 2020). Phosphate solubilization capacity is considered a key factor in isolating highly efficient PSBs from agricultural soils (Aliyat et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…However, the selection of an efficient PSB is crucial, as it practically increases P in the plant rhizosphere (Aliyat et al, 2020). Phosphate solubilization capacity is considered a key factor in isolating highly efficient PSBs from agricultural soils (Aliyat et al, 2020). Among 134 PSB strains from different leguminous plants' rhizosphere in the Fez-Meknes region, only 12 (12) PSBs show phenotypic stability of P solubilization after five successive subcultures in the NBRIP-RP plates.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco

2022

View full text Add to dashboard Cite

Low-cost and environmentally friendly agricultural practices have received increasing attention in recent years. Developing microbial inoculants containing phosphate (P) solubilizing bacteria (PSB) represents an emerging biological solution to improve rhizosphere P availability. The present study aims to explore PSB strains isolated from soils located at different bioclimatic stages in Morocco and present in various legumes rhizosphere to improve agronomic microbial fertilizer’s effectiveness. It was also aimed to test the isolated strains for their ability to solubilize P in NBRIP medium with Tricalcium P (Ca3 (PO4)2) (TCP), rock phosphate (RP), and their combination as a source of phosphorus, by (22) experiment design. Bacterial strains with a high P solubility index (PSI) were selected, characterized, and compared to commercial control. The vanadate-molybdate method was used to estimate P solubilization activity. Stress tolerance to salinity, acidity, drought, and temperature was tested. From all isolated strains (64), 12 were screened as promising biotechnological interest because of their P solubilization and their good resistance to different drastic conditions. Besides, the strain WJEF15 showed the most P solubility efficiency in NBRIP solid medium with a PSI of 4.1; while the WJEF61 strain was located as the most efficient strain in NBRIP-TCP liquid medium by releasing 147.62 mg.l–1 of soluble P. In contrast, in the NBRIP-RP medium, the strain WJEF15 presented maximum solubilization with 25.16 mg.l–1. The experiment design showed that a combination of RP and TCP with max level progressively increases P solubilization by 20.58%, while the WJEF63 strain has the most efficient concentration of 102.69 mg.l–1. Indeed, among the selected strains, four strains were able to limit tested fungi growth. Thus, results reveal a potential effect of selecting PSBs to support cropping cultures as plant growth-promoting rhizobacteria (PGPR).

show abstract

“…MPS is the most likely method for increasing plant-accessible P apart from treatment and enzymatic deterioration of natural mixes (Illmer and Schinner, 1992). MPS is the arrival of free P from insoluble mineral phosphates such as calcium phosphate (CaPO4), aluminium phosphate (AlPO4), or ferric phosphate (FePO4) via organic acid temperance (Aliyat et al 2022). PSM organic acid secretion is dependent on carbon (C) source accessibility, ecological conditions, metabolic state and physiology of the life form, and carbon catabolite repression (CCR) (Görke and Stülke, 2008).…”

Section: Phosphate Solubilizationmentioning

confidence: 99%

Review on Mechanism of Mineral Phosphate Solubilization in Fast-Growing Rhizobia Based on Sugar Utilization

Rajguru

Bhatt

2022

Plant Breed. Biotech.

View full text Add to dashboard Cite

Plant Growth Promoting Rhizobacteria (PGPR) were used in many previous studies for increasing plant growth and productivity. Some mechanisms were used to enhance the soil productivity such as mineral phosphate solubilization, siderophore production, biological nitrogen fixation, induced systemic resistance and indole acetic acid (IAA) production. Some of the related researches adopted the phosphate solubilization in organisms which was repressed in the presence of succinate resembling the phenomenon of catabolite repression. In addition, the sugar utilization pattern (monosaccharide, disaccharide, and trisaccharide), organisms showed the characteristics like slow and fast-growing rhizobia respectively. Whole-genome sequencing has been used for identifying unique genes in Fast-growing Rhizobia. Existing literature in PGPR studies has been considered and mechanisms for increasing plant growth and productivity have been identified. The review tries to analyse the mechanism of phosphate solubilization and sugar utilization in fast growing Rhizobia. On the contrary, repression due to various carbon sources such as succinate has been reported, leading to succinate mediated catabolite repression.

show abstract

Phosphate-Solubilizing Bacteria Isolated from Phosphate Solid Sludge and Their Ability to Solubilize Three Inorganic Phosphate Forms: Calcium, Iron, and Aluminum Phosphates

Cited by 46 publications

References 54 publications

Effect of pH and Carbon Source on Phosphate Solubilization by Bacterial Strains in Pikovskaya Medium

Effect of pH and Carbon Source on Phosphate Solubilization by Bacterial Strains in Pikovskaya Medium

Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco

Review on Mechanism of Mineral Phosphate Solubilization in Fast-Growing Rhizobia Based on Sugar Utilization

Contact Info

Product

Resources

About