Phosphate Solubilizing Fungi Isolated and Characterized from Teff Rhizosphere Soil Collected from North Showa and Gojam, Ethiopia

Abstract

been conducted by many researchers from soils, mangrove and rhizosphere [1,[12][13][14][15][16][17] AbstractPhosphorus (P) is one of the major bio elements limiting agricultural production. Phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. The present study was aimed at isolating and characterizing phosphate solubilizing fungi from teff rhizosphere soil. Fungi were identified using lactophenol cotton blue staining confirmation and Biolog micro station. Fungi isolates were screened and transferred to Biolog universal yeast agar media. Pure yeast cells and filamentous fungi were suspended in sterile water and filamentous fungi (FF) inoculum fluid at 49 ± 2 and 75 ± 2 turbidity measured by Biolog turbidimeter respectively. 100 μ-L transferred from each suspension into 96 wells of the biolog yeast micro Plate and filamentous fungi microplate tagged with different carbon source and incubated at 26°C for 24 to 72 h and read by the micro station reader at a single wavelength of 590 nm, results were recorded and processed for identification by micro log3 software ver. 4.20.05. Biolog micro station produce 24 fungi read results. Filamentous fungi ≤ 0.5 similarity index (62.5%), yeast ≥ 0.5 similarity index (25%), yeast ≤ 0.5 similarity index (12.5%). The identified fungi were evaluated for phosphate solubilization by the pikovskaya's agar (PVK) selective media. Seven species were positive in phosphate solubilizing ability.

Soil yeasts promoting plant growth: benefits for the development of common wheat and white mustard

“…During the phosphorus solubilization assay soil yeasts exhibited different modes of adaptation to given conditions resulting in different solubilization dynamics. But, at the bottom line, all of them confirmed the phosphorus solubilizing feature, which has already been documented for S. occidentalis and C. tropicalis (Al-Falih, 2005;Gizaw et al, 2017). This study represents the first report of C. saturnus ability to solubilize insoluble phosphorus.…”

Screening of Phosphate-Solubilizing Fungi From Air and Soil in Yunnan, China: Four Novel Species in Aspergillus, Gongronella, Penicillium, and Talaromyces

“…actinomyces, bacteria, fungi, and yeasts have been screened for phosphate solubilization. They were shown to enhance the solubilization of insoluble P compounds ( Gizaw et al, 2017 ; Nandimath et al, 2017 ; Mohamad et al, 2018 ; Wang et al, 2018 ; Chen and Liu, 2019 ; Liu et al, 2019 ). For fungi, Rhizopus stolonifer var.…”

Production and Optimization of Bio-Based Silica Nanoparticle from Teff Straw (Eragrostis tef) Using RSM-Based Modeling, Characterization Aspects, and Adsorption Efficacy of Methyl Orange Dye

“…Teff is farmed in most regions of Ethiopia; therefore, teff straw is readily available across the country [18]. Brown teff straw was gathered in Yebu, Jimma, Ethiopia, and sent to JIT, the School of Chemical Engineering laboratory, and the Faculty of Material Science and Engineering laboratory.…”