Phosphate transporters from the higher plant Arabidopsis thaliana.

Muchhal, Umesh S.; Pardo, José M.; Raghothama, Kashchandra G.

doi:10.1073/pnas.93.19.10519

Cited by 415 publications

(361 citation statements)

References 31 publications

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“…Published reports indicate that, in response to P deficiency, plants may increase synthesis of additional transporter molecules (Drew et al, 1984) or upregulate expression of genes encoding P transporters (Liu et al, 1998;Muchhal et al, 1996;Smith et al, 1997), leading to a subsequent increase in transporter proteins (Muchhal and Raghothama, 1999). The increase of As(V) uptake with P deficiency has been found by Wang et al (2002), which showed that 8 d of P starvation increases the maximal influx of As(V) by 2.5-fold, suggesting an increased density of P/As(V) transporters in the rhizoid cell plasma membranes.…”

Section: Scanning Sitementioning

confidence: 99%

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First evidence on different transportation modes of arsenic and phosphorus in arsenic hyperaccumulator Pteris vittata

Lei

Wan

Huang

et al. 2012

Environmental Pollution

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Section: Scanning Sitementioning

confidence: 99%

“…P has been considered to be acquired by plants through an energy-mediated transport process (Muchhal et al, 1996). Until now, there has been no direct study of the pattern of As transport into P. vittata rhizoids.…”

Section: Introductionmentioning

confidence: 99%

First evidence on different transportation modes of arsenic and phosphorus in arsenic hyperaccumulator Pteris vittata

Lei

Wan

Huang

et al. 2012

Environmental Pollution

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“…To monitor Piregulated gene expression, we used gene probes coding for an acid phosphatase (AtACP5), a Pi transporter (AtPT2), and a protein of unknown function (At4). These genes are induced by Pi limitation but are repressed in high Pi (Muchhal et al, 1996;del Pozo et al, 1999;Burleigh and Harrison, 1999). Seeds were germinated for 3 d on ϩPi medium and then transferred to ϩPi, ϪPi, and ϪPi/ϩPhi medium.…”

Section: Phi Selectively Represses Phosphate Starvationinducible Genementioning

confidence: 99%

“…RNA hybridization analysis with various 32 Plabeled probes, AtACP5 (del Pozo et al, 1999), At4 (Burleigh and Harrison, 1999), AtPT2 (Muchhal et al, 1996), and IAA1 and IAA3 (Abel et al, 1995), was carried out as described by Abel et al (1995). Equal RNA loading was assessed by hybridization with a rice (Oryza sativa) 17S ribosomal DNA probe.…”

Section: Isolation Of Nucleic Acids and Rna Hybridization Analysismentioning

confidence: 99%

Attenuation of Phosphate Starvation Responses by Phosphite in Arabidopsis

2001

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When inorganic phosphate is limiting, Arabidopsis has the facultative ability to metabolize exogenous nucleic acid substrates, which we utilized previously to identify insensitive phosphate starvation response mutants in a conditional genetic screen. In this study, we examined the effect of the phosphate analog, phosphite (Phi), on molecular and morphological responses to phosphate starvation. Phi significantly inhibited plant growth on phosphate-sufficient (2 mm) and nucleic acid-containing (2 mm phosphorus) media at concentrations higher than 2.5 mm. However, with respect to suppressing typical responses to phosphate limitation, Phi effects were very similar to those of phosphate. Phosphate starvation responses, which we examined and found to be almost identically affected by both anions, included changes in: (a) the root-to-shoot ratio; (b) root hair formation; (c) anthocyanin accumulation; (d) the activities of phosphate starvationinducible nucleolytic enzymes, including ribonuclease, phosphodiesterase, and acid phosphatase; and (e) steady-state mRNA levels of phosphate starvation-inducible genes. It is important that induction of primary auxin response genes by indole-3-acetic acid in the presence of growth-inhibitory Phi concentrations suggests that Phi selectively inhibits phosphate starvation responses. Thus, the use of Phi may allow further dissection of phosphate signaling by genetic selection for constitutive phosphate starvation response mutants on media containing organophosphates as the only source of phosphorus.Phosphorus is an essential structural constituent of many biomolecules and plays a pivotal role in energy conservation and metabolic regulation. Inorganic orthophosphate (Pi), the assimilated form of phosphorus, is often a limiting macronutrient in both terrestrial and aquatic ecosystems. As a consequence, assimilation, storage, and metabolism of Pi are highly regulated processes that directly affect plant growth (Theodorou and Plaxton, 1993;Raghothama, 1999). To cope with low Pi availability, plants have evolved sophisticated developmental and metabolic adaptations to enhance Pi acquisition from the rhizosphere. Such strategies include morphological changes in root architecture and associations with symbiotic mycorrhizal fungi to accelerate soil exploration as well as biochemical responses to chemically increase Pi availability from insoluble salt complexes and organophosphates present in recalcitrant soil matter (McCully, 1999;Raghothama, 1999). Despite numerous studies on adaptive responses to Pi limitation, little is known about the underlying molecular processes or regulatory genes that are involved in the Pi starvation response of plants.On the other hand, genetic and molecular studies have provided much insight into the microbial response to Pi limitation. When faced with low Pi availability, both Escherichia coli and Saccharomyces cerevisiae activate a multigene emergency rescue system to scavenge traces of usable phosphorus from the surrounding medium. Both systems are known as a p...

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“…This includes transporters from potato (StPT1 and StPT2) (Leggewie et al, 1997), Arabidopsis thaliana (AtPT1 and AtPT2) (Muchhal et al, 1996;, Medicago truncatula (MtPT1 and MtPT2) (Liu et al, 1998b) and tomato (LePT1 and LePT2) (Daram et al, 1998;Liu et al, 1998a). The encoded proteins share between 75 and 85% identity at the amino acid level and are distinct from PHT2;1.…”

Section: Introductionmentioning

confidence: 99%

The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface

Chiou¹,

Liu²,

Harrison³

2001

The Plant Journal

185

145

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SummaryThe movement of phosphate from the soil into plant root cells is the ®rst of many crucial transport events required to supply phosphorous (P) to cells throughout the plant. In addition to the ability to acquire phosphate from the soil, the majority of the vascular plants are able to form arbuscular mycorrhizal associations in which phosphate may be delivered to the cortex via a fungus. Previously, we cloned two phosphate transporter genes, MtPT1 and MtPT2 from Medicago truncatula roots. Complementation of a yeast phosphate transport mutant revealed that MtPT1 is a functional phosphate transporter and Northern analyses revealed that MtPT1 is expressed exclusively in roots (Liu et al., 1998, Mol. Plant-Microbe Interact. 11, 14±22). Utilising an antibody speci®c for MtPT1, we have analysed the accumulation and spatial expression patterns of the MtPT1 transporter. MtPT1 transcript and protein levels show close correlation and increase dramatically in the roots in response to phosphate starvation. MtPT1 protein levels decrease in roots during development of a symbiosis with arbuscular mycorrhizal (AM) fungi, indicating that this transporter is not involved in symbiotic phosphate transport. Membrane fractionation and analysis of a MtPT1/GFP fusion protein revealed that MtPT1 is located in the plasma membrane, while in situ hybridisation and immunolocalisation demonstrate the presence of MtPT1 transcripts and protein in the epidermal cells and root hairs of M. truncatula roots. MtPT1 shows expression patterns consistent with a role speci®cally in the acquisition of phosphate from the soil and is distinct from the other phosphate transporter of this class described to date.

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Phosphate transporters from the higher plant Arabidopsis thaliana.

Cited by 415 publications

References 31 publications

First evidence on different transportation modes of arsenic and phosphorus in arsenic hyperaccumulator Pteris vittata

First evidence on different transportation modes of arsenic and phosphorus in arsenic hyperaccumulator Pteris vittata

Attenuation of Phosphate Starvation Responses by Phosphite in Arabidopsis

The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface

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