Phosphatidic Acid in Plant Hormonal Signaling: From Target Proteins to Membrane Conformations

Kolesnikov, Yaroslav S.; Kretynin, S. V.; Bukhonska, Yaroslava; Pokotylo, Igor; Ruelland, Éric; Martinec, Jan; Kravets, V. S.

doi:10.3390/ijms23063227

Cited by 25 publications

(13 citation statements)

References 151 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Diacylglycerol kinases are key signalling enzymes that phosphorylate diacylglycerol to phosphatidic acid (PA), a pivotal second messenger also potentially associated with fruit ripening [ 29 ]. Phosphatidic acid is triggered by many plant stimuli [ 30 ], including abscisic acid (ABA), the plant hormone that directs ripening in nonclimacteric sweet cherry [ 31 ]. Thus, PA can mediate ABA-controlled fruit ripening, indicating the association of diacylglycerol kinase with fruit maturity deserves further investigation.…”

Section: Discussionmentioning

confidence: 99%

High-resolution genome-wide association study of a large Czech collection of sweet cherry (Prunus avium L.) on fruit maturity and quality traits

Holušová

Čmejlová

Suran

et al. 2022

Horticulture Research

View full text Add to dashboard Cite

In sweet cherry (Prunus avium L.), quantitative trait loci have been identified for fruit maturity, colour, firmness, and size to develop markers for marker-assisted selection. However, resolution is usually too low in those analyses to directly target candidate genes, and some associations are missed. In contrast, genome-wide association studies are performed on broad collections of accessions, and assemblies of reference sequences from Tieton and Satonishiki cultivars enable identification of single nucleotide polymorphisms after whole-genome sequencing, providing high marker density. Two hundred and thirty-five sweet cherry accessions were sequenced and phenotyped for harvest time and fruit colour, firmness, and size. Genome-wide association studies were used to identify single nucleotide polymorphisms associated with each trait, which were verified in breeding material consisting of 64 additional accessions. A total of 1,767,106 single nucleotide polymorphisms were identified. At that density, significant single nucleotide polymorphisms could be linked to co-inherited haplotype blocks (median size ~10 kb). Thus, markers were tightly associated with respective phenotypes, and individual allelic combinations of particular single nucleotide polymorphisms provided links to distinct phenotypes. In addition, yellow-fruit accessions were sequenced, and a ~90-kb-deletion on chromosome 3 that included five MYB10 transcription factors was associated with the phenotype. Overall, the study confirmed numerous quantitative trait loci from bi-parental populations using high-diversity accession populations, identified novel associations, and genome-wide association studies reduced the size of trait-associated loci from megabases to kilobases and to a few candidate genes per locus. Thus, a framework is provided to develop molecular markers and evaluate and characterize genes underlying important agronomic traits.

show abstract

Section: Discussionmentioning

confidence: 99%

High-resolution genome-wide association study of a large Czech collection of sweet cherry (Prunus avium L.) on fruit maturity and quality traits

Holušová

Čmejlová

Suran

et al. 2022

Horticulture Research

View full text Add to dashboard Cite

show abstract

“…Phosphatidic acid has been thoroughly studied in plant hormonal signaling 44 , and has been implicated in modulating cellular processes such as membrane traffic, endocytosis and exocytosis in mammals, but its precise role in these process is not well defined 45 .…”

Section: Discussionmentioning

confidence: 99%

Phosphatidic acid is an endogenous negative regulator of PIEZO2 channels and mechanical sensitivity

Gabrielle,

Yudin,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

Mechanosensitive PIEZO2 ion channels play roles in touch, proprioception, and inflammatory pain. Currently, there are no small molecule inhibitors that selectively inhibit PIEZO2 over PIEZO1. The TMEM120A protein was shown to inhibit PIEZO2 while leaving PIEZO1 unaffected. Here we find that TMEM120A expression elevates cellular levels of phosphatidic acid and lysophosphatidic acid (LPA), aligning with its structural resemblance to lipid-modifying enzymes. Intracellular application of phosphatidic acid or LPA inhibited PIEZO2, but not PIEZO1 activity. Extended extracellular exposure to the non-hydrolyzable phosphatidic acid and LPA analogue carbocyclic phosphatidic acid (ccPA) also inhibited PIEZO2. Optogenetic activation of phospholipase D (PLD), a signaling enzyme that generates phosphatidic acid, inhibited PIEZO2, but not PIEZO1. Conversely, inhibiting PLD led to increased PIEZO2 activity and increased mechanical sensitivity in mice in behavioral experiments. These findings unveil lipid regulators that selectively target PIEZO2 over PIEZO1, and identify the PLD pathway as a regulator of PIEZO2 activity.

show abstract

“…To estimate the influence of this lipid mediator, it was suggested to increase the level of proteins involved in the reversible binding of PA in plant tissues. Recently, searching for plant proteins that can bind to PA resulted in their identification in Arabidopsis and rice [41,54]. Among them, the sphingosine kinase 1 (SPHK1) contains a classical PA binding site, VSGDGI [41].…”

Section: The Influence Of Mtsphk1 Gene Fragment Encoding Domain For P...mentioning

confidence: 99%

Genetically Modified Legume Plants as a Basis for Studying the Signal Regulation of Symbiosis with Nodule Bacteria

Bovin,

Dolgikh,

Dymo

et al. 2023

Horticulturae

View full text Add to dashboard Cite

The development of legume–rhizobial symbiosis results in the formation of nitrogen-fixing root nodules. In response to rhizobial molecules, Nod factors, signal transduction is mediated by the interaction of activated receptors with downstream signaling proteins. Previously, some new regulators of the signal pathway, such as phospholipases D, which regulate the level of phosphatidic acid (PA), as well as mitogen-activated protein kinases (MAPKs), have been identified in legumes. Since PA is an important signal messenger, we tested the hypothesis that increasing the level of proteins involved in the reversible binding of PA in plant tissues may have a positive effect on symbiosis. Our findings showed that overexpression of MtSPHK1-PA, encoding the PA-binding domain of sphingosine kinase 1 (SPHK1), stimulated plant growth and nodule development in legume plants. Furthermore, the influence of MAPK6 on the development of symbiosis was studied. Using genetic engineering methods, we increased MAPK6 transcriptional activity in transgenic roots, leading to an increase in the number of nodules and the biomass of pea plants. Therefore, new approaches to obtain plants with an increased efficiency of symbiosis were tested. We report here that both genes that encode signaling proteins may be used as potential targets for future modification using biotechnological approaches.

show abstract

Phosphatidic Acid in Plant Hormonal Signaling: From Target Proteins to Membrane Conformations

Cited by 25 publications

References 151 publications

High-resolution genome-wide association study of a large Czech collection of sweet cherry (Prunus avium L.) on fruit maturity and quality traits

High-resolution genome-wide association study of a large Czech collection of sweet cherry (Prunus avium L.) on fruit maturity and quality traits

Phosphatidic acid is an endogenous negative regulator of PIEZO2 channels and mechanical sensitivity

Genetically Modified Legume Plants as a Basis for Studying the Signal Regulation of Symbiosis with Nodule Bacteria

Contact Info

Product

Resources

About