Phosphatidic acid liposomes as mineralizing surfaces: kinetics and energetics

Kraus, Betsy L.; Crenshaw, M.A.

doi:10.1016/s0009-2541(96)00054-x

Cited by 7 publications

(6 citation statements)

References 9 publications

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“…17 Moreover, Kraus and Crenshaw reported the interfacial energy for HA nucleation on phosphatidic acid liposomes to be 85.0 ergs/cm 2 . 34 These values are similar to the interfacial energy of HA seed crystals (91.0 ergs/cm 2 ). 26 The interfacial energies of covalently linked PV to agarose beads alone and in the presence of CMET confirmed that this narrow range (85-91 ergs/cm 2 ) in interfacial energy shows the nucleation potential of phosphorylated surface.…”

Section: Discussionsupporting

confidence: 75%

“…In that study, the interfacial energy for apatite nucleation on phosphophoryn covalently bound to collagen was determined to be 90.0 ergs/cm 2 17. Moreover, Kraus and Crenshaw reported the interfacial energy for HA nucleation on phosphatidic acid liposomes to be 85.0 ergs/cm 2 34. These values are similar to the interfacial energy of HA seed crystals (91.0 ergs/cm 2 ) 26.…”

Section: Discussionmentioning

confidence: 62%

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Effects of calcium salts of acidic monomers on mineral induction of phosphoprotein immobilized to agarose beads

Ito

Iijima

Motai

et al. 2012

J Biomedical Materials Res

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The aim of this study is to evaluate the mineralizing potential of acidic monomers and their calcium salts for mineralization, using an in vitro mineral induction model. Phosvitin (PV) was used as a model phosphoprotein in this study. PV was immobilized on agarose beads with divinyl sulfone. Five aliquots of agarose-immobilized PV, acidic monomers, and their calcium salts were incubated in mineralizing solution at various concentrations. The PV beads and acidic monomers were incubated at 37°C. Samples were taken at several time points during the incubation. Then, the agarose beads were analyzed for bound calcium by atomic absorption spectrometry. The mineral formed on the agarose beads was identified as an apatite by microarea X-ray diffraction. Additionally, the specimens were observed using scanning electron microscopy (SEM). Mineral induction time decreased with increasing solution saturation. 4-METCa salt [calcium salt of 4-methacryloxyethyl trimellitate (CMET)] significantly reduced the mineral induction time. Using these data, the interfacial tension for mineral induction of PV and CMET was determined to be 90.1 and 92.7 ergs/cm(2), respectively. The mineral induced in each specimen after incubation for 24 h was identified by its X-ray diffraction pattern as apatite. SEM observation showed that lath-shaped crystals were formed on the surfaces of the CMET. We conclude that CMET could play a role in dentin remineralization.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 62%

Effects of calcium salts of acidic monomers on mineral induction of phosphoprotein immobilized to agarose beads

Ito

Iijima

Motai

et al. 2012

J Biomedical Materials Res

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“…The interfacial tension calculated for dentin collagen was very similar to that calculated for phosphatidic acid liposomes (85 ergs/cm 2 ) (23) and for phosvitin cross-linked to agarose beads (91 ergs/cm 2 ) (Le, Kraus, and Crenshaw, unpublished data). The interfacial tension for hydroxyapatite nucleation on hydroxyapatite seed crystals (91 ergs/ cm 2 ) (24) is within this range.…”

Section: Figuresupporting

confidence: 67%

Apatite Induction by Insoluble Dentin Collagen

Saito¹,

Yamauchi²,

Crenshaw³

1998

Journal of Bone and Mineral Research

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Phosphoproteins are thought to play a role in mineral formation in dentin. A portion of this phosphoprotein is bound to collagen. We have investigated the requirement for bound phosphate in mineral induction by isolated dentin collagen. Insoluble bovine dentin collagen obtained by ethylene-diamino-tetra-acetic acid (EDTA) demineralization had 19.5 mol of P/mol of collagen that could not be extracted with 0.5 M EDTA in 4 M guanidine HCl. When this collagen was incubated in supersaturated solutions that did not spontaneously precipitate, apatite was induced. With progressive enzymatic dephosphorylation, induction times for mineral formation became progressively longer. The dentin did not induce mineral formation when 90% of the ester phosphate was removed. Insoluble bone collagen, which had even less phosphate, also did not induce mineral formation. Mineral induction times by dentin collagen increased with decreasing solution saturations. Using these data, the interfacial tension for mineral induction was determined to be 90 ergs/cm 2 . This value approximated that of phosphatidic acid liposomes and of phosvitin cross-linked to agarose beads, and it might reflect the energetics of heterogeneous nucleation on a highly phosphorylated surface. Sequestering of calcium-phosphate clusters on the phosphoprotein probably accounts for the observed calcium binding by dentin collagen in excess of that required to neutralize the phosphate esters of the collagen. Because the phosphoprotein is immobilized at a low density on the collagen, it cannot self-associate in calcium-phosphate solutions as it does when it is free in solution. This immobilized phosphoprotein allows the mineral clusters formed on its surface to grow into a crystalline order. (J Bone Miner Res 1998;13:265-270)

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“…These difficulties have successfully been tackled in former laboratory mineral nucleation studies under chemically well‐defined conditions with model compounds such as the nucleation of hydroxy‐apatite on artificial phosphatidic acid liposomes (e.g. Kraus & Crenshaw, 1996). By contrast, this study addresses the nucleation kinetics of calcite on the surface of living cyanobacterial cells (Obst et al.…”

Section: Introductionmentioning

confidence: 99%

CaCO₃ nucleation by cyanobacteria: laboratory evidence for a passive, surface‐induced mechanism

Obst¹,

2009

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Calcite nucleation on the surface of cyanobacteria of the Synechococcus leopoliensis strain PCC 7942 was investigated to assess the influence of photosynthetic uptake of inorganic carbon and active ion exchange processes across the cell membrane on the nucleation and precipitation mechanisms. We performed long-term precipitation experiments at a constant CO(2) level in ambient air by adding suspensions of previously washed cyanobacteria to solutions of NaHCO(3)/CaCl(2) which were supersaturated with respect to calcite. Induction times between 4 and 110 h were measured over a range of saturation states, Omega, between 8 and 4. The kinetics of CaCO(3) nucleation was compared between experiments: (i) with ongoing photosynthesis, (ii) with cells metabolizing but not undergoing photosynthetic uptake of inorganic carbon and (iii) in darkness without photosynthesis. No significant differences were observed between the three treatments. The results reveal that under low nutrient concentrations and permanent CO(2) supply, photosynthetic uptake of inorganic carbon predominantly uses CO(2) and consequently does not directly influence the nucleation process of CaCO(3) at the surface of S. leopoliensis. Furthermore, ion exchange processes did not affect the kinetics, indicating a passive nucleation process wherein the cell surface or extracellular polymers provided preferential sites for mineral nucleation. The catalyzing effect of the cyanobacteria on calcite nucleation was equivalent to a approximately 18% reduction in the specific interfacial free energy of the calcite nuclei. This result and the ubiquitous abundance of cyanobacteria suggest that this process may have an impact on local and global carbon cycling.

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Phosphatidic acid liposomes as mineralizing surfaces: kinetics and energetics

Cited by 7 publications

References 9 publications

Effects of calcium salts of acidic monomers on mineral induction of phosphoprotein immobilized to agarose beads

Effects of calcium salts of acidic monomers on mineral induction of phosphoprotein immobilized to agarose beads

Apatite Induction by Insoluble Dentin Collagen

CaCO₃ nucleation by cyanobacteria: laboratory evidence for a passive, surface‐induced mechanism

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Phosphatidic acid liposomes as mineralizing surfaces: kinetics and energetics

Cited by 7 publications

References 9 publications

Effects of calcium salts of acidic monomers on mineral induction of phosphoprotein immobilized to agarose beads

Effects of calcium salts of acidic monomers on mineral induction of phosphoprotein immobilized to agarose beads

Apatite Induction by Insoluble Dentin Collagen

CaCO3 nucleation by cyanobacteria: laboratory evidence for a passive, surface‐induced mechanism

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CaCO₃ nucleation by cyanobacteria: laboratory evidence for a passive, surface‐induced mechanism