Phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to intracellular adhesion molecule-1

Asai, Akira; Okajima, Fumitaka; Nakagawa, Kiyotaka; Ibusuki, Daigo; Tanimura, Kyoko; Nagao, Mototsugu; Sudo, Mariko; Harada, Taro; Miyazawa, Teruo; Oikawa, Shinichi

doi:10.1194/jlr.m800582-jlr200

Cited by 28 publications

(20 citation statements)

References 50 publications

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“…In another experiment, we found that PCOOH stimulated angiogenic responses in HUVECs (23). These previous findings (21)(22)(23) together with our recent results (24) will enhance our fundamental understanding of the involvement of PCOOH in Ox-LDL in the development of atherosclerosis, although further studies are needed to investigate atherogenic processes (e.g., cytotoxicity, apoptosis, mitotic catastrophe, ferroptosis, and angiogenesis) of PCOOH in Ox-LDL.…”

Section: Biological Roles Of Pcooh In Atherogenesissupporting

confidence: 71%

“…However, the biological mechanism has not been definitively established. We previously found that THP-1 cell adhesion to intracellular adhesion molecule-1 was increased in the presence of PCOOH (21,22). The result indicates that PCOOH in Ox-LDL may be involved in monocyte adherence to the arterial wall during the initiation of atherosclerosis.…”

Section: Biological Roles Of Pcooh In Atherogenesismentioning

confidence: 91%

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Determination of Phosphatidylcholine Hydroperoxide (PCOOH) as a Marker of Membrane Lipid Peroxidation

Nakagawa

Kato

Miyazawa

2015

J Nutr Sci Vitaminol

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In 1987, Miyazawa et al. established the methodology known as chemiluminescence detection-high performance liquid chromatography (CL-HPLC), which is both sensitive and selective enough to measure lipid-class hydroperoxide levels in foods, as well as in biological samples such as blood plasma and tissues (1-9). A unique post-column chemiluminescence reagent consisting of cytochrome c and luminol in an alkaline borate-buffered solution is commonly utilized for CL-HPLC. Using CL-HPLC, formation of mono-, bis-, and tris-hydroperoxides was found to occur during the initial stage of triacylglycerol oxidation in food oils (6, 7). Animal studies have shown that long-term fish oil consumption results in a high risk of membrane phospholipid peroxidation (i.e., abnormal accumulation of phosphatidylcholine hydroperoxide, PCOOH, together with an increased need for a-tocopherol as a membrane antioxidant (9). Moreover, membrane phospholipid peroxidation (PCOOH accumulation) increases with aging (senescence) (2). These findings strongly suggest the importance of determining lipid peroxidation levels in food and biological systems (refer to comprehensive reviews (10) for background information relating to lipid peroxidation). Pure Hydroperoxide Standards for QuantificationOne of the challenges faced by many researchers in measuring lipid hydroperoxides using CL-HPLC or other HPLC techniques is finding suitably pure hydroperoxide standards (11,12). Lipid hydroperoxides prepared from photo-or free radical-oxidized lipids are generally used as standard compounds. However, the purity of the hydroperoxides in such standards is not necessarily high (12). Impurities in the standards can be attributed to a number of analytical errors.Over 50 y ago it was reported that under acidic conditions, some vinyl ethers can react with organic hydroperoxides to form perketals (13,14). Thus, we predicted that this reaction could be applied for the preparation of pure lipid hydroperoxides, which was achieved using a vinyl-ether molecule (2-methoxypropene, MxP) (15). The procedures require protecting the hydroperoxide group as a perketal using MxP. The perketal is then separated using HPLC, and the pure hydroperoxide is regenerated from the perketal.The above-mentioned reactions are useful for the preparation of lipid hydroperoxides (e.g., PCOOH), although the reactions sometimes yield unknown brown byproducts. Hence, we recently reported a method to improve the reaction conditions, and demonstrated the synthesis of pure PCOOH without byproduct formation (16). In particular, the PCOOH isomer 1-palmitoyl-2-(9-hydroperoxyoctadecadienoyl)-sn-glycero-3-phosphocholine (16:0/9-HpODE PC) was synthetically prepared ( Fig. 1) as follows: linoleic acid was oxidized for 48 h at 40˚C. Among the resulting oxidation products, 9-hydroperoxyoctadecadienoic acid (9-HpODE) was chromatographically fractionated, and its hydroperoxide group was protected by MxP. The protected fatty acid was subsequently purified to yield 9-HpODE, in which the hydroperoxide group was prot...

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Section: Biological Roles Of Pcooh In Atherogenesissupporting

confidence: 71%

Section: Biological Roles Of Pcooh In Atherogenesismentioning

confidence: 91%

Determination of Phosphatidylcholine Hydroperoxide (PCOOH) as a Marker of Membrane Lipid Peroxidation

Nakagawa

Kato

Miyazawa

2015

J Nutr Sci Vitaminol

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“…Although the surface expression remained consistent in THP-1 monocytes, the conformation changed to the high-affinity conformation, which may explain the increase in adhesion of ␤-hydroxybutyrate-treated cells, since neither LFA-1 nor ICAM-1 surface expression was increased with ␤-hydroxybutyrate treatment. Previous studies in the literature have also reported that, when treated with phosphatidylcholine hydroperoxide, the expression of LFA-1 does not increase in THP-1 monocytes, but the activation state changes, resulting in increased adhesion to ICAM-1 (4). These data suggest that physiological concentrations of both acetoacetate and ␤-hydroxybutyrate may result in upregulation and activation of adhesion molecules as a way to recruit an increased number of monocytes to the vascular endothelium.…”

Section: E301 Hyperketonemia Induces Monocyte-endothelial Cell Adhesionmentioning

confidence: 54%

Hyperketonemia increases monocyte adhesion to endothelial cells and is mediated by LFA-1 expression in monocytes and ICAM-1 expression in endothelial cells

Rains

Jain

2011

American Journal of Physiology-Endocrinology and Metabolism

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Rains JL, Jain SK. Hyperketonemia increases monocyte adhesion to endothelial cells and is mediated by LFA-1 expression in monocytes and ICAM-1 expression in endothelial cells. Am J Physiol Endocrinol Metab 301: E298 -E306, 2011. First published May 3, 2011 doi:10.1152/ajpendo.00038.2011.-Frequent episodes of hyperketonemia are associated with a higher incidence of vascular disease. The objective of this study was to examine the hypothesis that hyperketonemia increases monocyte-endothelial cell (EC) adhesion and the development of vascular disease in diabetes. Human U937 and THP-1 monocyte cell lines and human umbilical vein endothelial cells (HUVECs) were cultured with acetoacetate (AA) (0 -10 mM) or ␤-hydroxybutyrate (BHB) (0 -10 mM) for 24 h prior to evaluating adhesion and adhesion molecule expression. The results demonstrate a significant (P Ͻ 0.01) increase in both U937 and THP-1 adhesion to HUVEC monolayers treated with 4 mM AA compared with control. Equal concentrations of BHB resulted in similar increases in monocyte-EC adhesion. Similarly, treatments of AA or BHB to isolated monocytes from human blood also show increases in adhesion to endothelial cells. intercellular adhesion molecule-1 (ICAM-1) was significantly increased on the surface of HUVECs and an increase in total protein expression with AA treatment compared with control. The expression level of lymphocyte function-associated antigen-1 (LFA-1) was increased in monocytes treated with AA, and LFA-1 affinity was altered from low to high affinity following treatment with both AA and BHB. Monocyte adhesion could be blocked when cells were preincubated with an antibody to ICAM-1 or LFA-1. Results also show a significant increase in IL-8 and MCP-1 secretion in monocytes and HUVECs treated with 0 -10 mM AA. These results suggest that hyperketonemia can induce monocyte adhesion to endothelial cells and that it is mediated via increased ICAM-1 expression in endothelial cells and increased expression and affinity of LFA-1 in monocytes.intercellular adhesion molecule-1; lymphocyte function-associated antigen-1; ketosis; type 1 diabetes; inflammation; adhesion molecules VASCULAR INFLAMMATION and cardiovascular disease (CVD) are the leading causes of mortality and morbidity among patients with diabetes. Type 1 diabetes (T1D) is associated with increased vascular complications and is considered a proinflammatory condition (8). Studies in the current literature indicate that T1D is associated with increased oxidative stress and increased levels of various inflammatory biomarkers such as CRP, monocyte activity, sICAM, sE-selectin, sP-selectin, and sCD40L (9,10,20). The current literature also contains reports of an increased risk for CVD in T1D (12,29,34). In addition to hyperglycemia, T1D patients frequently experience hyperketonemia due to a state of insulin deficiency. T1D is the most common pathological cause of elevated ketone bodies; however, it has also been shown to coexist with hyperglycemia among older T2D patients and in select minority groups with...

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“…Reactive oxygen species (ROS) generation was measured using 5-(and-6)-carboxy-2Ј,7Ј-difluorodihydrofluorescein diacetate (carboxy-H 2 DFFDA; Invitrogen) as described previously. 30 In brief, cells were washed in PBS, treated with 5M 5-(and-6)-carboxy-2Ј,7Ј-difluorodihydrofluorescein diacetate for 30 minutes at 37°C, and washed twice with PBS. The resulting fluorescence was analyzed by flow cytometry.…”

Section: Sod2 Activity and Ros Measurementmentioning

confidence: 99%

Platelets and platelet-like particles mediate intercellular RNA transfer

Risitano

Beaulieu

Vitseva

et al. 2012

Blood

180

154

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Phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to intracellular adhesion molecule-1

Cited by 28 publications

References 50 publications

Determination of Phosphatidylcholine Hydroperoxide (PCOOH) as a Marker of Membrane Lipid Peroxidation

Determination of Phosphatidylcholine Hydroperoxide (PCOOH) as a Marker of Membrane Lipid Peroxidation

Hyperketonemia increases monocyte adhesion to endothelial cells and is mediated by LFA-1 expression in monocytes and ICAM-1 expression in endothelial cells

Platelets and platelet-like particles mediate intercellular RNA transfer

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