2009
DOI: 10.1021/ja808431h
|View full text |Cite
|
Sign up to set email alerts
|

Phosphatidylcholine “Wobble” in Vesicles Assessed by High-Resolution 13C Field Cycling NMR Spectroscopy

Abstract: High resolution 13C NMR field cycling (covering 11.7 down to 0.002 T) relaxation studies of the sn-2 carbonyl of phosphatidylcholines in vesicles provide a detailed look at the dynamics of this position of the phospholipid in vesicles. The spin-lattice relaxation rate, R1, observed down to 0.05 T is the result of dipolar and CSA relaxation components characterized by a single correlation time τc, with a small contribution from a faster motion contributing CSA relaxation. At lower fields, R1 increases further w… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

1
27
0

Year Published

2009
2009
2023
2023

Publication Types

Select...
6
1

Relationship

1
6

Authors

Journals

citations
Cited by 16 publications
(28 citation statements)
references
References 12 publications
1
27
0
Order By: Relevance
“…In order to preserve the spin polarization as much as possible, the magnetic field could be maintained above 0.1 T during the 'voyage' by enclosing the transfer tube in permanent or electro-magnets. Our observations also indicate that hyperpolarized NMR or MRI experiments of nuclei such as 13 C or 31 P should be performed at moderate fields to prevent CSA relaxation in high fields [25][26][27].…”
Section: Introductionmentioning
confidence: 65%
See 2 more Smart Citations
“…In order to preserve the spin polarization as much as possible, the magnetic field could be maintained above 0.1 T during the 'voyage' by enclosing the transfer tube in permanent or electro-magnets. Our observations also indicate that hyperpolarized NMR or MRI experiments of nuclei such as 13 C or 31 P should be performed at moderate fields to prevent CSA relaxation in high fields [25][26][27].…”
Section: Introductionmentioning
confidence: 65%
“…Conventional relaxometers using variations of the current that drives the electro-magnet [18,21,22] are usually designed for 1 H, and do not offer reasonable sensitivity for less sensitive low-c spins such as 13 C. More elaborate relaxometers using superconducting magnets and involving shuttling of the sample into a second magnet [23], or moving the entire probe assembly in the stray field [24], provide ways to conveniently address low-c spins [25][26][27]. We have developed a simple and inexpensive mechanical 'shuttle' (Fig.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…collective dynamic processes [8]. There has recently been resurgence in the application of this technique in the field of membrane biophysics, whereby high-resolution 31 P NMR and 13 C NMR have been used by Redfield and coworkers to extract dynamic parameters for small phospholipid vesicles [124126]. Moreover, this technique is used extensively in the study of proteins that continue to extend the view of these dynamic systems [127132].…”
Section: Nmr Relaxation Gives Dynamical Parameters That Characterimentioning
confidence: 99%
“…Though the monitoring of 31 P nuclei restricts the system to small multicomponent vesicles, monitoring of 13 Ccarbonyl-labeled phospholipids (19) would enable the use of large unilamellar vesicles and more biologically complex membranes. The methodology also should be applicable to non-lipid problems where ligands (large or small but containing 31 P (20), or 13 C without directly bonded protons (19)) in fast exchange with large complexes can be used to map out distances to specific spin-labeled probes on the macromolecule.…”
Section: Pi-plcmentioning
confidence: 99%