1995
DOI: 10.1074/jbc.270.22.13225
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Phosphatidylinositol 3-Kinase Activity Is Required at a Postendocytic Step in Platelet-derived Growth Factor Receptor Trafficking

Abstract: We have previously reported that platelet-derived growth factor (PDGF) receptor mutants that lack high affinity binding sites for phosphatidylinositol 3-kinase (PI 3-kinase) fail to concentrate in juxtanuclear vesicular structures after activation with PDGF. We have now identified the point in the endocytic pathway at which PI 3-kinase binding sites are required. Receptor internalization from the plasma membrane, measured as the acquisition of acid resistance of prebound 125I-PDGF, was only slightly decreased … Show more

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Cited by 159 publications
(135 citation statements)
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“…5). This finding is consistent with previous studies on the effects of PI 3-kinase inhibitors on the degradation of other signaling receptors, such as the PDGF receptor [16] and the EGF receptor [25,26] There are at least three PI 3-kinases in mammalian cells, and the most commonly used PI 3-kinase inhibitors act on all three [27]. Evidence from other studies implicates the type III PI 3-kinase hVps34 in controlling traffic to lysosomes [28,29].…”
Section: Discussionsupporting
confidence: 91%
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“…5). This finding is consistent with previous studies on the effects of PI 3-kinase inhibitors on the degradation of other signaling receptors, such as the PDGF receptor [16] and the EGF receptor [25,26] There are at least three PI 3-kinases in mammalian cells, and the most commonly used PI 3-kinase inhibitors act on all three [27]. Evidence from other studies implicates the type III PI 3-kinase hVps34 in controlling traffic to lysosomes [28,29].…”
Section: Discussionsupporting
confidence: 91%
“…4). β 2 AR degradation is slowed by LY294002 PI 3-kinase inhibitors reduce the rate of degradation of some internalized growth factor receptors [16,17]. During prolonged exposure to agonist, β 2 ARs undergo downregulation, a loss of receptors that correlates with their movement through late endosomes and into lysosomes [9,10].…”
Section: The Effect Of Ly294002 On Transferrin Recyclingmentioning
confidence: 99%
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“…In mammalian cells, PI 3-kinase has also been implicated in mediating endocytotic pathway for membrane proteins (Joly et al, 1994). PI 3-kinase activity is necessary for the proper delivery of the activated PDGF receptors to lysosomes (Joly et al, 1995;Shpetner et al, 1996). In contrast, wortmannin did not block the TPA-induced endocytosis of Ecadherin and c-Met and cell scattering.…”
Section: Discussionmentioning
confidence: 96%
“…PtdIns(3,4,5)P 3 , 1 and the polyphosphoinositide 3-kinase that synthesizes this lipid both feature prominently in this area (1,2). For example, intracellular trafficking of tyrosine kinase receptors depends upon interactions between the internalized receptors and the 3-kinase (3). Additionally, PtdIns(3,4,5)P 3 interacts either directly or indirectly with several GTPases that play important roles in vesicle traffic (1).…”
mentioning
confidence: 99%