Phosphatidylserine Metabolism in Human Lymphoblastic Cells Exposed to Chromium (VI)

Gambelunghe, Angela; Buratta, Sandra; Ferrara, Giuseppina; Mozzi, Rita; Marchetti, Cristina; Murgia, Nicola; Muzi, Giacomo

doi:10.1097/jom.0b013e31821f2aaf

Cited by 2 publications

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References 26 publications

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“…Changes in lipid composition in cells undergoing apoptosis were mostly investigated using radioactive labeling, as shown in previous studies evaluating PS synthesis in apoptotic cells [25][26][27][28][29]. However, results obtained with this approach do not necessarily resemble the metabolic activities of non-manipulated cells [30,31], as they may reflect changes due to the administration of a radioactive precursor leading to activation/inhibition of the specific metabolic pathway under study.…”

Section: Introductionmentioning

confidence: 99%

Changes in Lipid Composition During Manganese-Induced Apoptosis in PC12 Cells

et al. 2015

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Lipid composition of membranes is fundamental to modulate signaling pathways relying on lipid metabolites and/or membrane proteins, thus resulting in the regulation of important cell processes such as apoptosis. In this case, membrane remodeling is an early event important for the activation of signaling leading to cell death and removal of apoptotic cells. In the present study, we analyzed phospholipid, cholesterol and fatty acid content during apoptosis induced by manganese in PC12 cells. Lipid analysis of whole cells and detergent-resistant membranes was carried out by HPLC/GC. Results showed that apoptosis is associated with changes in lipid composition detectable in whole cell extracts, namely cholesterol, phosphatidylserine and phosphatidylethanolamine decreases. Noteworthy, phosphatidylserine level reduction was detectable before to the detection of apoptosis, in correlation with our previous study carried out by radioactive labelling. By contrast, phosphatidylserine and phosphatidylethanolamine changes were not detected in detergent resistant membranes, which instead showed an altered composition in phosphatidylinositol, phosphatidylcholine and sphingomyelin in apoptotic cells.

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Section: Introductionmentioning

confidence: 99%