Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae

Loera-Quezada, Maribel M.; Leyva‐González, Marco Antonio; López‐Arredondo, Damar; Herrera-Estrella, Luis

doi:10.1016/j.plantsci.2014.11.015

Cited by 44 publications

(38 citation statements)

References 52 publications

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“…Because Phi is not metabolized by algae, algal bloom issues might be lessened in freshwater systems receiving P from farm runoff or tile drain leaching. The compound does not appear to be lethal to algae but inhibits growth through competition with OP much like the observations reported on terrestrial experiments (Loera‐Quezada et al, 2015). The rate of microbial oxidation from Phi to OP will likely dictate the efficacy with respect to this facet of the proposed system.…”

Section: Alternative Phosphorus Sourcessupporting

confidence: 72%

A Review of the Latest in Phosphorus Fertilizer Technology: Possibilities and Pragmatism

2019

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The development of highly concentrated phosphorus (P) fertilizers, such as triple superphosphate, by the Tennessee Valley Authority helped mark the beginning of a revolution in the way we manage food crop nutrition. Since then, scientists, with the help of farmers, have made great advancements in the understanding of P fate and transport across many environments but largely have failed to produce a new generation of products and/or application techniques that are widely accepted and that vastly improve plant acquisition efficiency. Under certain conditions, important advancements have been made. For example, applying liquid formulations of phosphates in lieu of dry granules in some highly calcareous soils has dramatically reduced precipitation as sparingly soluble calcium phosphate minerals, but other attempts, such as the co‐application of humic substances, sorption to layered double hydroxides, or use of nanoparticles, have not generated the kind of results necessary to continue economically increasing crop yields without further environmental cost. New sources of fertility will need to be affordable to produce, transport, and furnish P to soil solution in a manner well synchronized with crop demand. This paper provides a review of recent literature on cutting‐edge phosphorus fertilizer technology. The goal is that this synthesis will be used as a starting point from which a larger discussion on responsible nutrient management and increased P use efficiency research can be built. Core Ideas Reaction with some soil constituents limits P availability and crop yield. A variety of approaches to improve fertilizer use efficiency are being explored. Ideally, P availability should be well synchronized to crop demand. More innovation along with mechanistic and field‐scale trials is required.

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Section: Alternative Phosphorus Sourcessupporting

confidence: 72%

A Review of the Latest in Phosphorus Fertilizer Technology: Possibilities and Pragmatism

2019

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“…Previously, we reported that some microalgae (i.e. ; Botryococcus braunii and Ettlia oleoabundans ) were unable to metabolize Phi as a P source (Loera‐Quezada et al ., ). As part of this work, we determined that other microalgae including Haematococcus pluvialis and S. obliquus were also unable to metabolize Phi (Figure S3).…”

Section: Resultsmentioning

confidence: 97%

“…For this purpose, we tested the growth of cells harvested from media containing 1 mM Pi (P-replete cells) or from media devoid of a P source (P-starved cells) in medium supplemented with 0.1 mM Phi as P source in a Multicultivator photobioreactor (MC 1000, Qubit Systems; Qubit Biology Inc. Kingston, Ontario, Canada) ( Figure 1a). We tested Preplete and P-starved cells because we have previously observed that cells grown in 1 mM Pi are able to sustain significant growth in media lacking a P source (Loera-Quezada et al, 2015). We observed that after 6 days of cultivation in media containing Phi as a sole P source the putative ptxD transgenic lines were all capable of growing with a growth rate similar to that of the WT or the same transgenic lines in media containing Pi as a P source ( Figure 1; compare panel b versus c).…”

Section: Generation and Characterization Of Transgenic Chlamydomonas mentioning

confidence: 99%

“…In contrast to prokaryotes, no evidence exists suggesting that eukaryotic organisms have the required genes for the metabolism of any P reduced compound. In fact, it has been extensively documented that plants cannot metabolize Phi (Ávila et al ., ; Berkowitz et al ., ) and more recently, we showed that a number of microalgae species, including Chlamydomonas reinhardtii , Ettlia oleoabundans and Botryococcus braunii, are unable to use Phi as a sole P source (Loera‐Quezada et al ., ). To develop microalga that can be used to design systems to prevent undesirable contaminations in open or closed cultivation systems, we designed a system to make cells able to metabolize Phi, a nonmetabolizable compound, as a sole P source.…”

Section: Introductionmentioning

confidence: 97%

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A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae

Loera-Quezada

Leyva‐González

Velázquez‐Juárez

et al. 2016

Plant Biotechnology Journal

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SummaryMicroalgal cultivation that takes advantage of solar energy is one of the most cost‐effective systems for the biotechnological production of biofuels, and a range of high value products, including pharmaceuticals, fertilizers and feed. However, one of the main constraints for the cultivation of microalgae is the potential contamination with biological pollutants, such as bacteria, fungi, zooplankton or other undesirable microalgae. In closed bioreactors, the control of contamination requires the sterilization of the media, containers and all materials, which increases the cost of production, whereas open pond systems severely limits the number of species that can be cultivated under extreme environmental conditions to prevent contaminations. Here, we report the metabolic engineering of Chlamydomonas reinhardtii to use phosphite as its sole phosphorus source by expressing the ptxD gene from Pseudomonas stutzeri WM88, which encodes a phosphite oxidoreductase able to oxidize phosphite into phosphate using NAD as a cofactor. Engineered C. reinhardtii lines are capable of becoming the dominant species in a mixed culture when fertilized with phosphite as a sole phosphorus source. Our results represent a new platform for the production of microalgae, potentially useful for both closed photobioreactors and open pond systems without the need for using sterile conditions nor antibiotics or herbicides to prevent contamination with biological pollutants.

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“…La ausencia de efectos tóxicos de los fosfitos sobre el fitoplancton ya había sido demostrada con anterioridad. Concentraciones entre 7.9 y 158.17 mg/L de fosfito potásico monobásico no presentaron efectos tóxicos que causaran mortalidad de las células para la microalga Chlamydomonas reinhardtii (Loera-Quezada et al 2015). En el caso de la cianobacteria Microcystis aeruginosa, se ha observado que el fosfito parece ser inerte y no tóxico hasta concentraciones de 500 mg/L (Zhang et al 2011).…”

Section: Discussionunclassified

Análisis Ecotoxicológico Del Fungicida Fosfito De Sodio Sobre Una Comunidad Planctónica

Parra

Acevedo-García

Valcarcel

et al. 2019

Rev. Int. Contam. Ambie.

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Palabras clave: ecotoxicología, microcosmos, zooplancton, agroquímico RESUMEN La agricultura es una actividad estratégica de la sociedad que ocasiona impactos negativos sobre el ambiente, algunos relacionados con el uso excesivo de productos de síntesis para el control de plagas o la fertilización química. El fungicida fosfito de sodio presenta datos de baja toxicidad bajo condiciones estándar de laboratorio y organismos tipo ensayo. Sin embargo, no se han realizado ensayos ecotoxicológicos que permitan ayudar en la evaluación del riesgo ambiental de esta sustancia. El presente trabajo estudia el efecto de la exposición de larga duración (60 días) del fungicida fosfito de sodio sobre la comunidad planctónica, a través de ensayos con microcosmos. Para la determinación de las concentraciones a utilizar en los microcosmos se realizó un ensayo preliminar uniespecífico crónico de exposición con Daphnia magna en laboratorio. Después de evaluar los resultados de dicho experimento, se decidió que las concentraciones de exposición en los microcosmos fueran 5 y 10 mg/L. Los resultados de los microcosmos indican que no han tenido lugar efectos negativos, asociado al tratamiento en términos estructurales (abundancia y composición taxonómica) de la comunidad zooplanctónica. Además, la comunidad de productores primarios, fitoplancton y perifiton, tampoco mostró respuesta negativa, en términos de cambios en abundancia, a dicha exposición. Los resultados indican que la utilización de fosfito a estas concentraciones no representa un riesgo para la comunidad zooplanctónica bajo estudio. Sin embargo, la falta de información sobre los efectos en la estructura del componente fitoplanctónico debe hacer primar el principio de precaución en el uso de esta sustancia.

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Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae

Cited by 44 publications

References 52 publications

A Review of the Latest in Phosphorus Fertilizer Technology: Possibilities and Pragmatism

A Review of the Latest in Phosphorus Fertilizer Technology: Possibilities and Pragmatism

A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae

Análisis Ecotoxicológico Del Fungicida Fosfito De Sodio Sobre Una Comunidad Planctónica

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