2021
DOI: 10.1021/acs.biomac.1c00085
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Phosphocholine-Decorated PPI-Dendrimers Mimic Cell Membrane Phosphocholine Clusters and Tune the Innate Immune Activity of C-Reactive Protein

Abstract: C-reactive protein (CRP) is a widely used as biomarker of infection and inflammation. It has a well-described ability to bind phosphocholine (PC), as well as PC-clusters from compromised and inflamed cell membranes and tissues. The binding of PC-clusters to CRP is of interest as this binding determines subsequent innate immune activity. We investigated PC-decorated dendrimers as mimics for PC-clusters. Five generations of poly(propylene imine) (PPI) dendrimers were modified with PC surface groups via a three-s… Show more

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“…The mean CRP level in the blood under physiological conditions is 0.1–10 mg/L but will elevate to thousand times during cell damage or inflammation, and the half-life of CRP in serum is about 19 h. Rapidly and precisely, detecting the changes in CRP concentration is vital for monitoring the disease progresses and prognosis. A diverse range of methodologies have been developed for CRP sensing, including fluorescence, SPR, , SERS, quantum dots, , and electrochemical and optical approaches. They are all generally important and sensitive for the CRP test with a low detection limit, while these design strategies are generally indirect measurements combined with specific interactions between CRP and aptamer or antibody. , Recently, Paolo Actis’s group employed the DNA origami nanopore for CRP sensing via aptamer binding, and the modeling of CRP in the microchannel has been done as well . Unlike the reported work, we introduced a nanopore-based technique for direct label-free identification of the conformations of CRP and its aptamer-binding complex, at the single-molecule level with a detection limit as low as 0.3 ng/μL.…”
Section: Introductionmentioning
confidence: 99%
“…The mean CRP level in the blood under physiological conditions is 0.1–10 mg/L but will elevate to thousand times during cell damage or inflammation, and the half-life of CRP in serum is about 19 h. Rapidly and precisely, detecting the changes in CRP concentration is vital for monitoring the disease progresses and prognosis. A diverse range of methodologies have been developed for CRP sensing, including fluorescence, SPR, , SERS, quantum dots, , and electrochemical and optical approaches. They are all generally important and sensitive for the CRP test with a low detection limit, while these design strategies are generally indirect measurements combined with specific interactions between CRP and aptamer or antibody. , Recently, Paolo Actis’s group employed the DNA origami nanopore for CRP sensing via aptamer binding, and the modeling of CRP in the microchannel has been done as well . Unlike the reported work, we introduced a nanopore-based technique for direct label-free identification of the conformations of CRP and its aptamer-binding complex, at the single-molecule level with a detection limit as low as 0.3 ng/μL.…”
Section: Introductionmentioning
confidence: 99%