Phosphodiesterase type 4 inhibitor rolipram inhibits activation of monocytes during extracorporeal circulation

Satô, Yukio; Hiramatsu, Yuji; Homma, Satoshi; Sato, M; Sato, Shyoko; Endo, Shunsuke; Sohara, Yasunori

doi:10.1016/j.jtcvs.2004.12.028

Cited by 11 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent investigations, it has been accepted that membrane‐anchored rTFs are quite similar to the natural TF . Our previous studies have demonstrated that simulated CPB activates platelets, neutrophils, and monocytes, and promotes the formation of microaggregates within 30 minutes after starting simulated CPB . Moreover, platelet activation is known to be initiated rapidly after starting CPB.…”

Section: Discussionmentioning

confidence: 98%

Early Heparin Administration Attenuates Tissue Factor-Mediated Thrombin Generation During Simulated Cardiopulmonary Bypass

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 98%

Early Heparin Administration Attenuates Tissue Factor-Mediated Thrombin Generation During Simulated Cardiopulmonary Bypass

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Monocytes were in direct contact with a synthetic surface during the experimental procedure but were not specifically treated or activated; nonetheless, this extracorporeal contact resulted in monocytes activation 28, 29. Using microfluidic methods, the extracorporeal human monocytes strongly adhered to autologous ICAM-1 and to mouse ICAM-1 even without additional treatment.…”

Section: Discussionmentioning

confidence: 99%

Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery

et al. 2018

View full text Add to dashboard Cite

Background: The accurate determination of liver segment anatomy is essential to perform liver resection without complications and to ensure long-term outcomes after this operation. There are several perioperative methods for segment identification and surgical navigation, such as intraoperative ultrasound, indigo carmine and near-infrared imaging with indocyanine green. The present study experimentally analyzed the usefulness of monocyte sequestration for liver segment labeling and imaging.Methods: Human monocytes were isolated from peripheral blood and directly or indirectly labeled with calcein or IRDye 800CW. Potential toxicity, labeling stability, and adhesion to ICAM-1 were analyzed in vitro. Monocyte sequestration in the liver microvasculature and liver segment labeling and boundary demarcation were studied using isolated mouse and pig liver perfusion and via intraportal injection in mouse liver tumor models.Results: The highest monocyte labeling efficiency was achieved using direct labeling with IRDye 800CW. Labeling was stable and did not influence cell viability. The labeled monocytes were highly sequestrated in the liver microvasculature, both after ex vivo perfusion and after injection in vivo , resulting in excellent labeling of selected liver segments and strong segment boundary demarcation. In contrast to results to a normal liver, monocyte sequestration was very low in tumor-associated blood vessels.Conclusions: The present experimental study shows that sequestration of labeled monocytes after superselective application demarcates the selected liver segment. These results illustrate potential of this technique for surgical navigation during liver surgery.

show abstract

“…The attraction of monocytes to activated platelets is hypothesized to be through the secretion of the monocyte chemoattractant protein-1 (MCP-1) which has been recently shown to be markedly induced by activated platelets [62]. The monocyte-platelet interaction activates the monocyte into a proinflammatory state and upregulation of the surface integrin, CD11b mediates the firm adhesion to platelets [12,63]. Our results show a significant increase in monocyte CD11b upon exposure to ECC and NO released from the polymer-coated ECC reduced this enhanced CD11b expression.…”

Section: Discussionmentioning

confidence: 99%

The attenuation of platelet and monocyte activation in a rabbit model of extracorporeal circulation by a nitric oxide releasing polymer

et al. 2010

View full text Add to dashboard Cite

Nitric oxide (NO) has been shown to reduce thrombogenicity by decreasing platelet and monocyte activation by the surface glycoprotein, P-selectin and the integrin, CD11b, respectively. In order to prevent platelet and monocyte activation with exposure to an extracorporeal circulation (ECC), a nitric oxide releasing (NORel) polymeric coating composed of plasticized polyvinyl chloride (PVC) blended with a lipophilic N-diazeniumdiolate was evaluated in a 4 hour rabbit thrombogenicity model using flow cytometry. The NORel polymer significantly reduced ECC thrombus formation compared to polymer control after 4 hours blood exposure (2.8 ± 0.7 NORel vs 6.7 ± 0.4 pixels/cm2 control). Platelet count (3.4 ± 0.3 NORel vs 2.3 ± 0.3 × 108/ml control) and function as measured by aggregometry (71 ± 3 NORel vs 17 ± 6 % control) were preserved after 4 hours exposure in NORel versus control ECC. Plasma fibrinogen levels significantly decreased in both NORel and control groups. Platelet P-selectin mean fluorescence intensity (MFI) as measured by flow cytometry was attenuated after 4 hours on ECC to ex vivo collagen stimulation (27 ± 1 NORel vs 40 ± 2 MFI control). Monocyte CD11b expression was reduced after 4 hours on ECC with NORel polymer (87 ± 14 NORel vs 162 ± 30 MFI control). These results suggest that the NORel polymer coatings attenuate the increase in both platelet P-selectin and monocytic CD11b integrin expression in blood exposure to ECCs. These NO-mediated platelet and monocytic changes were shown to improve thromboresistance of these NORel-polymer-coated ECCs for biomedical devices.

show abstract

Phosphodiesterase type 4 inhibitor rolipram inhibits activation of monocytes during extracorporeal circulation

Cited by 11 publications

References 30 publications

Early Heparin Administration Attenuates Tissue Factor-Mediated Thrombin Generation During Simulated Cardiopulmonary Bypass

Early Heparin Administration Attenuates Tissue Factor-Mediated Thrombin Generation During Simulated Cardiopulmonary Bypass

Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery

The attenuation of platelet and monocyte activation in a rabbit model of extracorporeal circulation by a nitric oxide releasing polymer

Contact Info

Product

Resources

About