Phosphodiesterases and subcellular compartmentalized cAMP signaling in the cardiovascular system

Stangherlin, Alessandra; Zaccolo, Manuela

doi:10.1152/ajpheart.00766.2011

Cited by 62 publications

(51 citation statements)

References 147 publications

(105 reference statements)

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“…Interestingly, what emerges from the work by Krawutchke et al is that the regulation of cGMP pools varies in different cell types and largely depends on the PDE isoforms those cells express. 12 Indeed, although in rat neonatal cardiomyocytes, the cGMP generated by the soluble guanylyl cyclase is controlled by PDE5 and PDE2, 13 in aortic smooth muscle cells, it is controlled almost exclusively by PDE5. On the same lines, the cGMP pool generated by the particulate guanylyl cyclase is controlled preferentially by PDE2 in cardiomyocytes 13 and by both PDE5 and PDE3 in aortic smooth muscle cells.…”

Section: See Accompanying Article On Page 2011mentioning

confidence: 99%

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Stangherlin

Zoccarato

2015

ATVB

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Section: See Accompanying Article On Page 2011mentioning

confidence: 99%

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Stangherlin

Zoccarato

2015

ATVB

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“…cAMP) (Baillie, 2009;Cooper, 2003;Houslay, 2010;Stangherlin and Zaccolo, 2012), as well as through structural factors, such as cellular compartments and scaffold proteins (Dodge-Kafka et al, 2006), which establish local microdomains that contain varying concentrations of these molecules. To probe how local cAMP concentrations affect the specificity of cAMP-PKA signaling, we developed the aforementioned method for generating site-specific cAMP signals using soluble adenylyl cyclase (Sample et al, 2012).…”

Section: Light-inducible Perturbation Toolsmentioning

confidence: 99%

Genetically encoded molecular probes to visualize and perturb signaling dynamics in living biological systems

Sample

Mehta

Zhang

2014

Journal of Cell Science

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In this Commentary, we discuss two sets of genetically encoded molecular tools that have significantly enhanced our ability to observe and manipulate complex biochemical processes in their native context and that have been essential in deepening our molecular understanding of how intracellular signaling networks function. In particular, genetically encoded biosensors are widely used to directly visualize signaling events in living cells, and we highlight several examples of basic biosensor designs that have enabled researchers to capture the spatial and temporal dynamics of numerous signaling molecules, including second messengers and signaling enzymes, with remarkable detail. Similarly, we discuss a number of genetically encoded biochemical perturbation techniques that are being used to manipulate the activity of various signaling molecules with far greater spatial and temporal selectivity than can be achieved using standard pharmacological or genetic techniques, focusing specifically on examples of chemically driven and light-inducible perturbation strategies. We then describe recent efforts to combine these diverse and powerful molecular tools into a unified platform that can be used to elucidate the molecular details of biological processes that may potentially extend well beyond the realm of signal transduction.

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“…Intracellular cAMP concentrations are regulated and shaped by a superfamily of proteins called PDEs (phosphodiesterases) [19,20]. There are 11 families of PDEs and eight of them can degrade cAMP.…”

Section: The Camp Signalling Machinerymentioning

confidence: 99%

cAMP signalling meets mitochondrial compartments

Lefkimmiatis

2014

Biochemical Society Transactions

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Mitochondria are highly dynamic organelles comprising at least three distinct areas, the OMM (outer mitochondrial membrane), the IMS (intermembrane space) and the mitochondrial matrix. Physical compartmentalization allows these organelles to host different functional domains and therefore participate in a variety of important cellular actions such as ATP synthesis and programmed cell death. In a surprising homology, it is now widely accepted that the ubiquitous second messenger cAMP uses the same stratagem, compartmentalization, in order to achieve the characteristic functional pleiotropy of its pathway. Accumulating evidence suggests that all the main mitochondrial compartments contain segregated cAMP cascades; however, the regulatory properties and functional significance of such domains are not fully understood and often remain controversial issues. The present mini-review discusses our current knowledge of how the marriage between mitochondrial and cAMP compartmentalization is achieved and its effects on the biology of the cell.

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Phosphodiesterases and subcellular compartmentalized cAMP signaling in the cardiovascular system

Cited by 62 publications

References 147 publications

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Genetically encoded molecular probes to visualize and perturb signaling dynamics in living biological systems

cAMP signalling meets mitochondrial compartments

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