Phosphoglucomutase Activity as a Novel Biomarker in Patients With Acute Myocardial Infarction

Abstract

Background: Phosphoglucomutase (PGM), a key enzyme in cellular glucose utilization and energy homeostasis, has been reported to show a relationship with oxidative stress. However, the clinical importance of PGM activity has not been investigated in patients with ischemic heart disease (IHD). The aim of the present pilot study was to clarify whether PGM activity has potential as a cardiovascular risk predictor in patients with IHD. Methods and Results:The levels of serum PGM activity in 237 patients with IHD (63 patients with acute myocardial infarction (AMI) and 174 patients with stable effort angina pectoris (EAP)) were evaluated. PGM activity was compared with levels of various myocardial, thrombosis, and inflammatory biomarkers on admission. PGM activity in the AMI group was significantly increased relative to that in the EAP group on admission (AMI, 55.5 μmol · min -1 · L -1 (U/L); EAP, 14.4 U/L (P<0.001)), and was observed to increase in parallel with well-established myocardial markers (P<0.001). Moreover, PGM activity and the lipid, thrombosis, and inflammatory biomarkers in the AMI group were higher than those in the EAP group.Conclusions: PGM activity increased with levels of myocardial, thrombosis, and inflammatory biomarkers in patients with AMI, and might be useful in diagnostic applications during the acute phase in patients with AMI. (Circ J 2012; 76: 2197 - 2203)

Proteomics‐Based Identification of the Molecular Signatures of Liver Tissues from Aged Rats following Eight Weeks of Medium‐Intensity Exercise

“…In addition, Preisler et al [41] implied that the liver might be affected by PGM deficiency and that reduced liver glucose levels might contribute to the low blood glucose levels observed during exercise in this condition. In this regard, polymorphism in PGM has been reported to be useful as a genetic biomarker for alcoholic cirrhosis [42], ischemic heart disease [43], and obesity disorders [44, 45]. The results of the current study show that training exercise increased the protein content of PGM in the livers of old rats, which likely contributed to the improved glycolytic metabolism of these animals, despite their advanced cirrhosis.…”

Need and Importance of Biomarkers to Predict Acute Myocardial Infarction and Its Prognosis

“…16 Therefore, PGM activity might indicate myocardial damage, thrombus formation, and the presence of inflammation associated with coronary plaque rupture. Their pilot study suggested that PGM activity, including that of the PGM isoform, might have useful diagnostic applications in the acute phase of AMI patients, although further studies would be required to elucidate the findings in detail.…”

A simple and rapid method for measuringα-D-phosphohexomutases activity by using anion-exchange chromatography coupled with an electrochemical detector

“…PGM catalyze the interconvertion of glucose-1-phosphate (Glc-1-P) and Glc-6-P. The normal way to detect the activity of PGM was using Glc-1-P as substrates, glucose-6-phosphate dehydrogenase (G6PDH) as coupled enzyme, by detect the increasing absorbance values of NADH at 340 nm ( Nishinari et al, 2012 ; Wang & Zhang, 2010 ). This method was convenience and simple because G6PDH was commercially available and cost-effective.…”