Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells

Wang, Han; Hu, Mingyue; Ding, Zhaoxue; Zhou, Xiao‐Long; Yang, Songbai; Shen, Zhonghao; Yan, Feifei; Zhao, Ayong

doi:10.1016/j.psj.2022.101805

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…Given that muscular increase of PHGDH has been described in the context of both regeneration 19 and initiation of apoptosis via the caspase‐3 pathway, 20 additional co‐immunofluorescence studies were carried out utilising antibodies targeting neonatal myosin (muscle regeneration marker) and caspase‐3 (apoptosis marker). These studies revealed a co‐localisation of PHGDH dot‐like sarcoplasmic structures (often enriched at the sarcolemma) with caspase‐3.…”

Section: Resultsmentioning

confidence: 99%

“…Given that muscular increase of PHGDH has been described in the context of both regeneration 19 (Figure S2). Co-immunofluorescence studies of BET1 and p62 revealed a pronounced immunoreactivity of p62 in the neurogenic targets of skeletal muscle fibres derived from patients with idiopathic NMA.…”

Section: Abundance and Distribution Of Blood Biomarker Proteins In Vw...mentioning

confidence: 87%

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Proteomic studies in VWA1‐related neuromyopathy allowed new pathophysiological insights and the definition of blood biomarkers

Athamneh,

Daya,

Hentschel

et al. 2024

J Cellular Molecular Medi

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Bi‐allelic variants in VWA1, encoding Von Willebrand Factor A domain containing 1 protein localized to the extracellular matrix (ECM), were linked to a neuromuscular disorder with manifestation in child‐ or adulthood. Clinical findings indicate a neuromyopathy presenting with muscle weakness. Given that pathophysiological processes are still incompletely understood, and biomarkers are still missing, we aimed to identify blood biomarkers of pathophysiological relevance: white blood cells (WBC) and plasma derived from six VWA1‐patients were investigated by proteomics. Four proteins, BET1, HNRNPDL, NEFM and PHGDH, known to be involved in neurological diseases and dysregulated in WBC were further validated by muscle‐immunostainings unravelling HNRNPDL as a protein showing differences between VWA1‐patients, healthy controls and patients suffering from neurogenic muscular atrophy and BICD2‐related neuromyopathy. Immunostaining studies of PHGDH indicate its involvement in apoptotic processes via co‐localisation with caspase‐3. NEFM showed an increase in cells within the ECM in biopsies of all patients studied. Plasma proteomics unravelled dysregulation of 15 proteins serving as biomarker candidates among which a profound proportion of increased ones (6/11) are mostly related to antioxidative processes and have even partially been described as blood biomarkers for other entities of neuromuscular disorders before. CRP elevated in plasma also showed an increase in the extracellular space of VWA1‐mutant muscle. Results of our combined studies for the first time describe pathophysiologically relevant biomarkers for VWA1‐related neuromyopathy and suggest that VWA1‐patient derived blood might hold the potential to study disease processes of clinical relevance, an important aspect for further preclinical studies.

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Section: Resultsmentioning

confidence: 99%

Section: Abundance and Distribution Of Blood Biomarker Proteins In Vw...mentioning

confidence: 87%

Proteomic studies in VWA1‐related neuromyopathy allowed new pathophysiological insights and the definition of blood biomarkers

Athamneh,

Daya,

Hentschel

et al. 2024

J Cellular Molecular Medi

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show abstract

“…In addition, PHGDH is also related to the proliferation and differentiation of chicken muscle cells. PHGDH can promote the proliferation and differentiation of primary chicken myoblasts, inhibit myoblast proliferation by knocking down PHGDH with siRNA, and overexpression of PHGDH enhances muscle cell proliferation ( Chen et al, 2022b ; Wang et al, 2022 a).…”

Section: Discussionmentioning

confidence: 99%

Characterization and comparative transcriptomic analysis of skeletal muscle in female Pekin duck and Hanzhong Ma duck during different growth stages using RNA-seq

Cao,

Cai,

et al. 2023

Poultry Science

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“…Loss of the NOV gene in mice causes muscle wasting ( Heath et al, 2008 ). Our previous study indicated that PHGDH promoted the proliferation of chicken skeletal muscle satellite cells ( Wang et al, 2022 ). TNNT2 is an important gene that induces grouper myoblasts differentiation ( Kong et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of the differentially expressed genes in the leg muscles of Zhedong white geese (Anser cygnoides) reared under different photoperiods

HangFeng

et al. 2022

Poultry Science

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Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells

Cited by 7 publications

References 29 publications

Proteomic studies in VWA1‐related neuromyopathy allowed new pathophysiological insights and the definition of blood biomarkers

Proteomic studies in VWA1‐related neuromyopathy allowed new pathophysiological insights and the definition of blood biomarkers

Characterization and comparative transcriptomic analysis of skeletal muscle in female Pekin duck and Hanzhong Ma duck during different growth stages using RNA-seq

Identification of the differentially expressed genes in the leg muscles of Zhedong white geese (Anser cygnoides) reared under different photoperiods

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