Phospholipase C zeta 1 mRNA as a marker of oocyte-activation and fertilization potential of water buffalo (Bubalus bubalis) semen

Atabay, Eufrocina P.; Fajardo, Z.P.; Tadeo, R.D.; Atabay, Edwin C.; Venturina, Emma V.; Mingala, Claro N.; Fissore, Rafael A.

doi:10.1016/j.livsci.2019.04.011

Cited by 3 publications

(6 citation statements)

References 32 publications

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“…There is evidence that the PLCZ1 protein is essential to trigger oocyte activation ( Ramadan et al, 2012 ; Kashir et al, 2018 ) and that the higher the levels of PLCZ1 in the spermatozoa, the higher the percentage of oocyte fertilization ( Aghajanpour et al, 2011 ; Atabay et al, 2019 ). As observed in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Raramuri Criollo bulls have good semen quality throughout the year and high testosterone concentrations ( Quezada-Casasola et al, 2018 ), however, ensuring semen quality measured in the conventional way does not guarantee embryo viability ( Franco et al, 2020 ). Therefore, in recent years, in addition to the conventional analyses of seminal quality and viability, important biochemical and physiological indicators for achieving successful pregnancy are being implemented ( Atabay et al, 2019 ; Krishnaiah et al, 2019 ; Franco et al, 2020 ). These analyses might include molecular biomarkers such as: metabolites, enzymes and RNAs ( Le Blévec et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…PLCZ1 mRNA is specific to spermatids ( Ramadan et al, 2012 ) and a positive relationship has been found between the remnants of this mRNA and the amount of PLCZ1 protein within the spermatozoon, in addition to a possible further functionality ( Aghajanpour et al, 2011 ). PLCZ1 mRNA levels are higher in more fertile individuals, compared to the less fertile ones in human males ( Aghajanpour et al, 2011 ), buffaloes ( Atabay et al, 2019 ) and Holstein bulls ( Kasimanickam et al, 2012 ). The objective of this study was to evaluate whether, under a low-input production system, the expression of PLCZ1 mRNA in sperm cells varies between breeds (Raramuri Criollo and Europeans) and times of the year (winter, summer).…”

Section: Introductionmentioning

confidence: 99%

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Expression of PLCZ1 mRNA in spermatozoa of Criollo and European bulls in a low-input system

Ronquillo-Roacho,

Rodriguez-Almeida,

Antillón-Ruiz

et al. 2023

Anim. Reprod.

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Sperm motility and kinematics analysis are important to predict bull fertility. However, there are other molecules in the sperm with the ability to improve the pregnancy rate. For example, PLCZ1 is a sperm protein that plays a unique role in the activation of the zygote and is important for the survival of the embryo. The objective of this work was to compare the expression of PLCZ1 mRNA in sperm cells of Chihuahuan Criollo and European bulls in the winter and summer seasons, under a low-input system. Six (3.33 ± 0.43 years old) bulls (three Criollo, three European) were used. Gross and individual motility were measured in semen obtained by electrostimulation. The cell pack was pelletized by centrifugation and stored in liquid nitrogen. The sperm cells were purified and total RNA was extracted. cDNA was synthesized to perform qPCR and measure the relative level of PLCZ1 transcripts in each bull. There were no differences in individual motility, however, gross motility was lower (P < 0.05) in Criollo bulls, both in the winter (71.1 ± 2.8 vs. 76.6 ± 2.8%) and in the summer season (58.9 ± 2.8 vs. 77.7 ± 2.8%). PLCZ1 expression was 5.3 times higher (P < 0.05) in winter than in summer (5.09 ± 1.09 vs 0.959 ± 1.09). No difference (P>0.05) was found in the expression levels of PLCZ1 between both breeds (4.36 ± 1.09 vs 1.69 ± 1.09), for Criollo and European, respectively. Although the animals presented seminal motility within the recommended limits for insemination, the expression levels of PLCZ1 vary depending on the time of the year and this might impact the rate of successful pregnancies. Therefore, it is important to complement conventional analysis of seminal quality with molecular characteristics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of PLCZ1 mRNA in spermatozoa of Criollo and European bulls in a low-input system

Ronquillo-Roacho,

Rodriguez-Almeida,

Antillón-Ruiz

et al. 2023

Anim. Reprod.

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“…To overcome problems of container volumes, several devices have been applied for buffalo MII oocyte vitrification by using very small amounts of solution and submerging the sample quickly into the LN 2 (Table 6). This includes SSV (Atabay et al, 2013;Boonkusol et al, 2007;Gasparrini et al, 2007;Liang, Rakwongrit, et al, 2012), Cryoloop (Gasparrini et al, 2007), Cryotop (Atabay et al, 2013;Attanasio, Boccia, et al, 2010;Liang, Rakwongrit, et al, 2012;Muenthaisong et al, 2007;Wang et al, 2016), straws (Gautam et al, 2008), and microdrop (Liang, Srirattana, et al, 2012). Cryotop has been successfully applied for IVM oocytes and SCNT blastocysts (Laowtammathron et al, 2005;Parnpai et al, 2004Parnpai et al, , 2016.…”

Section: Cryopreservation Of Matured Buffalo Oocytesmentioning

confidence: 99%

“…On the type of permeable cryoprotectants, a mixture of EG and DMSO has been widely used for buffalo MII oocyte vitrification (Atabay et al, 2013; Attanasio, Boccia, et al, 2010; Gautam et al, 2008; Liang, Srirattana, et al, 2012; Muenthaisong et al, 2007). The exposure time in CPAs is considered a critical factor that requires balancing between preventing the formation of intracellular ice and preventing toxic injury.…”

Section: Cryopreservation Of Oocytes and Embryosmentioning

confidence: 99%

Current status of assisted reproductive technologies in buffaloes

Srirattana

Hufana-Durán

Atabay

et al. 2022

Animal Science Journal

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Buffaloes are raised by small farm holders primarily as source of draft power owing to its resistance to hot climate, disease, and stress conditions. Over the years, transformation of these animals from draft to dairy was deliberately carried out through genetic improvement program leading to the development of buffalo-based enterprises. Buffalo production is now getting more attention and interest from buffalo raisers due to its socioeconomic impact as well as its contribution to propelling the livestock industry in many developing countries. Reproduction of buffaloes, however, is confronted with huge challenge and concern as being generally less efficient to reproduce compared with cattle due to both intrinsic and extrinsic factors such as poor estrus manifestation, silent heat, marked seasonal infertility, postpartum anestrus, long calving interval, delayed puberty, inherently low number of primordial follicles in their ovaries, high incidence of atresia, and apoptosis. Assisted reproductive technologies (ARTs) are major interventions for the efficient utilization of follicle reserve in buffaloes. The present review focuses on estrus and ovulation synchronization for fixed time artificial insemination, in vitro embryo production, intracytoplasmic sperm injection, cryopreservation of oocytes and embryos, somatic cell nuclear transfer, the factors affecting utilization in various ARTs, and future perspectives in buffaloes.

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Future of reproductive biotechnologies in water buffalo in Southeast Asian countries

Hufana-Duran,

Chaikhun-Marcou,

Duran

et al. 2025

Theriogenology

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Phospholipase C zeta 1 mRNA as a marker of oocyte-activation and fertilization potential of water buffalo (Bubalus bubalis) semen

Cited by 3 publications

References 32 publications

Expression of PLCZ1 mRNA in spermatozoa of Criollo and European bulls in a low-input system

Expression of PLCZ1 mRNA in spermatozoa of Criollo and European bulls in a low-input system

Current status of assisted reproductive technologies in buffaloes

Future of reproductive biotechnologies in water buffalo in Southeast Asian countries

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