Phospholipase D activity of Corynebacterium pseudotuberculosis (Corynebacterium ovis) and Corynebacterium ulcerans, a distinctive marker within the genus Corynebacterium

Barksdale, Lane; Linder, Regina; Sulea, I T; Pollice, M. C.

doi:10.1128/jcm.13.2.335-343.1981

Cited by 54 publications

(22 citation statements)

References 33 publications

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“…The reproducibility of the PFGE method was confirmed by repeated testing of a panel of five isolates. 2,3,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44 Inserts prepared from the same batch of plugs, when run on eight different gels, yielded identical banding patterns. The in vitro stability of the pulsotypes was confirmed in that the banding patterns before and after 20 passages were identical.…”

Section: Resultsmentioning

confidence: 99%

Characterization of United Kingdom Isolates ofCorynebacterium pseudotuberculosisUsing Pulsed-Field Gel Electrophoresis

et al. 2000

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Caseous lymphadenitis is a chronic suppurative disease caused byCorynebacterium pseudotuberculosis and is responsible for serious economic losses to the sheep and goat industry. Caseous lymphadenitis was first reported for goats in the United Kingdom in 1990 and for sheep in 1991. Recent evidence suggests that the prevalence of the disease within the national flock is increasing. Fifty isolates of C. pseudotuberculosis from the United Kingdom comprising sheep and horse isolates, the original goat outbreak strain, and the type strain were characterized by biotyping, antimicrobial susceptibility, production of phospholipase D, and genotyping by pulsed-field gel electrophoresis using SfiI and SmaI. All of the isolates were confirmed as C. pseudotuberculosis, and all produced phospholipase D but none reduced nitrate. Restriction with SfiI generated 16 to 18 bands between 48.5 and 290 kb and differentiated six pulsotypes. We conclude that 80% of the strains tested were epidemiologically related to the outbreak strain and that the equine profile was distinct both phenotypically and genotypically.

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Section: Resultsmentioning

confidence: 99%

Characterization of United Kingdom Isolates ofCorynebacterium pseudotuberculosisUsing Pulsed-Field Gel Electrophoresis

et al. 2000

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“…Diphtheria toxin production was demonstrated by means of the Elek test, using C. diphtheria strain NCTC 10648 and NCTC 10356 as controls. Phospholipase D activity was demonstrated by cooperative production of haemolysis on sheep blood plates with phospholipase C, produced by C.equi (1). The strain inhibited the interaction between P-lysin of Xuureus and CAMP protein of streptococcus group B with sheep erythrocytes (I).…”

Section: Case Reportmentioning

confidence: 99%

INFECTION DUE TO “CORYNEBACTERIUM ULCERANS”, PRODUCING DIPHTHERIA TOXIN

Pers

1987

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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“…The exotoxin, which is produced by all known isolates of the organism, is a phospholipase D (PLD); many of its biological properties remain unclear. It is known that PLD hydrolyzes lysophosphatidylcholine and sphingomyelin (2,29), has a pl of about 9. 8, requires calcium and magnesium ions for activity, is toxic for laboratory rodents and domestic animals (14), and lyses sheep erythrocytes in synergy with cholesterol oxidase and phospholipase C (equi factors) produced by Rhodococcus equi (2,3,29).…”

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confidence: 99%

“…It is known that PLD hydrolyzes lysophosphatidylcholine and sphingomyelin (2,29), has a pl of about 9. 8, requires calcium and magnesium ions for activity, is toxic for laboratory rodents and domestic animals (14), and lyses sheep erythrocytes in synergy with cholesterol oxidase and phospholipase C (equi factors) produced by Rhodococcus equi (2,3,29). Reported molecular weights of PLD, as determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, vary widely (10, 16,25).…”

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confidence: 99%

Cloning and expression of the phospholipase D gene from Corynebacterium pseudotuberculosis in Escherichia coli

et al. 1990

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A toxic phospholipase D (PLD) is putatively involved in pathogenesis of Corynebacterium pseudotuberculosis infections. We report here the cloning and expression of the PLD gene (pld) in Escherichia coli. A cosmid library of DNA from C. pseudotuberculosis biovar ovis isolate Whetten 1 was constructed and screened for PLD-producing recombinants by plating them on LB agar containing sheep erythrocytes and equi factors. One recombinant, designated pCpOl, yielded a gene product which displayed synergistic hemolytic and sphingomyelinase D activities, both of which are characteristic of PLD. Subcloning into pUC19 yielded a recombinant, pCpO5O, which contained a 1.8-kilobase insert. Analysis of supernatant fluids and cell extracts of cultures of E. coli(pCpO5O) revealed sphingomyelinase activity and a protein of about 31,000 Mr, neither of which were detected in E. coli(pUC19). The 31-kilodalton protein also reacted with antibodies in serum from a sheep naturally infected with C. pseudotuberculosis, serum which also contained PLD-neutralizing antibodies. When Southern blots of BamHI digests of DNA from biovar ovis and biovar equi isolates of C. pseudotuberculosis were probed with pCpO50, bands of 4.8 and 1.9 kilobases, respectively, were seen, suggesting that the genome organization of pid is different for isolates from the two biovars. * Corresponding author. t Arizona Technical Paper no. 4274, a contribution of the Arizona Agricultural Experiment Station, with the approval of the director.

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Phospholipase D activity of Corynebacterium pseudotuberculosis (Corynebacterium ovis) and Corynebacterium ulcerans, a distinctive marker within the genus Corynebacterium

Cited by 54 publications

References 33 publications

Characterization of United Kingdom Isolates ofCorynebacterium pseudotuberculosisUsing Pulsed-Field Gel Electrophoresis

Characterization of United Kingdom Isolates ofCorynebacterium pseudotuberculosisUsing Pulsed-Field Gel Electrophoresis

INFECTION DUE TO “CORYNEBACTERIUM ULCERANS”, PRODUCING DIPHTHERIA TOXIN

Cloning and expression of the phospholipase D gene from Corynebacterium pseudotuberculosis in Escherichia coli

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