1995
DOI: 10.1006/abio.1995.1234
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Phospholipid/Alkanethiol Bilayers for Cell-Surface Receptor Studies by Surface Plasmon Resonance

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Cited by 185 publications
(136 citation statements)
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“…This value is similar to the values obtained for self-assembled octadecanethiol monolayer on silver [70] (117 • ), and on gold [71] (105 • ) surfaces.…”
Section: Methodssupporting
confidence: 88%
“…This value is similar to the values obtained for self-assembled octadecanethiol monolayer on silver [70] (117 • ), and on gold [71] (105 • ) surfaces.…”
Section: Methodssupporting
confidence: 88%
“…These results demonstrate that the liquid ordered lipid bilayer is very efficient in preventing undesired physical adsorption without the need of using blocking buffer or other agents. It has been reported a partial ability of a lipid film to suppress nonspecific adsorption of proteins [33,34] in other substrates, namely poly(dimethylsiloxane) [35], poly(methyl methacrylate) [33], silicon [34] and even gold [36]. However, the extent of suppression of the liquid ordered bilayer used is virtually complete.…”
Section: Resultsmentioning
confidence: 99%
“…A variety of additional immobilization techniques are available in addition those described below, such as hydrazide coupling (O'Shannessy et al, 1992), Ni 2+ chelate coupling (Gershon and Khilko, 1995;O'Shannessy et al, 1995;Sigal et al, 1996), coupling of hydrophobic groups (Stein and Gerisch, 1996), coupling of azide-containing ligands with strain-promoted azide-alkyne cycloaddition (Wammes et al, 2013), the use of selfassembled monolayers of alkanethiolates with different coupling groups such as NTA (Sigal et al, 1996), biotin (Jung et al, 2000), maleimide (Houseman et al, 2003), aldehyde (Hahn et al, 2007), acetylene (Lee et al, 2004), hydroquinone (Yousaf and Mrksich, 1999), or supported lipid or hybrid bilayers (Plant et al, 1995;Ramsden et al, 1996), and immobilization to aminosilane-derivatized surfaces (Edwards et al, 1995;Buckle et al, 1993). For details on chemical modifications of the proteins, see (Hermanson, 2013).…”
Section: Immobilization Protocolsmentioning
confidence: 99%