Phospholipid and phospholipid fatty acid composition of mixed murine spinal cord neuronal cultures

Abstract

The phospholipid and phospholipid fatty acid compositions of mixed murine spinal cord neuronal cultures are reported. The phospholipid composition was primarily comprised of ethanolamine glycerophospholipids (44.8%) and choline glycerophospholipids (43.5%). Plasmalogens made up 29.1% of the ethanolamine glycerophospholipids (13.0% of the total phospholipids) and 4.5% of the choline glycerophospholipids (1.9% of the total phospholipids). Other phospholipids ranged from 2.9% for sphingomyelin to 1.0% for phosphatidylinositol 4-phosphate. The fatty acid compositions of the ethanolamine glycerophospholipids, choline glycerophospholipids, phosphatidylserine, and phosphatidylinositol were also determined. The choline glycerophospholipids were the most saturated and contained the smallest amount of polyunsaturated fatty acids. The ethanolamine glycerophospholipids were the most unsaturated and contained the highest amount of polyunsaturated fatty acids. The phospholipids contained minimal amounts of 20:3 n-9 (Mead acid) and are not considered polyunsaturated fatty acid deficient. Thus, for the mixed neuronal spinal cord cultures, the phospholipid fatty acid compositions were not polyunsaturated fatty acid deficient and contained a large amount of polyenoic fatty acids of both the n-3 and n-6 series.

Influence of Myo-inositol Plus Ethanolamine on Plasmalogens and Cell Viability during Oxidative Stress

“…PE-Pls are 3 times more abundant than PC-Pls and accounted for 75% of the plasmalogen pool. The contribution of PE-Pls to the PL pool (12.2%) is nearly identical to that measured in mixed murine spinal cord neuronal cultures . However, PE-Pls in the latter system are a smaller fraction of total ethanol­amine phospho­lipids (29.1%) compared to our findings in N2A cells (49%).…”

In Vitro Growth Environment Produces Lipidomic and Electron Transport Chain Abnormalities in Mitochondria from Non-Tumorigenic Astrocytes and Brain Tumours

“…The effect of passage number, culture conditions and cellular differentiation on fatty acid composition has been extensively studied in transformed and primary cell lines (Murphy and Horrocks, 1993; Murphy et al, 1993, 1997; Cheng et al, 2008). However, no prior studies have examined the influence of growth environment on the mitochondrial lipidome and ETC activities in brain tumour cell lines compared with syngeneic non-tumorigenic astrocytes grown under identical conditions (Springer, 1980; Kunz-Schughart et al, 2001).…”

Electrospray ionization tandem mass spectrometry of glycerophosphoethanolamine plasmalogen phospholipids

“…Glycerophosphoethanolamine plasmalogens, which are acid labile, were hydrolyzed by exposure to 12N HCl fumes [13]. This was achieved by drying down normal phase fractions containing GPEtn plasmalogens under a stream of nitrogen and exposing the GPEtn lipids to hydrochloric acid fumes for 1 h. This caused hydrolysis of the labile alkenyl ether bond of the plasmalogens while the 1-O-alkyl-2-acyl-and diacyl-GPEtn remained intact.…”